聚乳酸微孔膜的表面相容性修饰及透析性能研究

发布时间：2018-02-27 19:00

本文关键词： 聚乳酸血液透析膜亲水性透析性能血液相容性　出处：《青岛科技大学》2017年硕士论文　论文类型：学位论文

【摘要】：聚乳酸(PLA)作为一种生物基聚合物,具有良好的成膜性、可控降解、结构易调控以及附加值高等优点。但是PLA微孔膜的表面抗污性以及生物相容性需要进一步提高,以满足其在生物医用领域如透析方面的潜在应用。本课题主要采用非溶剂诱导相分离法制备PLA微孔膜,通过表面微溶胀及界面交联的方式在膜表面引入亲水性链段(如PVP链段),提高其亲水抗污性能,并进一步通过表面修饰抗凝基团(如肝素、水蛭素以及类肝素),从而提高PLA微孔膜的血液相容性。主要研究内容如下:肝素固定聚乳酸膜的制备及其血液相容性研究;水蛭素氢键固定聚乳酸膜的制备及抗凝血性研究;类肝素修饰聚乳酸微孔膜的血液相容性研究。通过对聚乳酸微孔膜的物理和化学形态结构、亲水性、血液相容性以及透析性能等方面进行研究,结论如下:(1)通过酰胺化反应及吸附将肝素固定到PLA膜表面,提高了PLA膜表面的血液相容性。首先通过酰胺化反应合成Hep-APTES化合物,然后将Hep-APTES通过自缩聚反应以及多层吸附等方式固定到PLA膜表面,从而制备出肝素化修饰PLA膜。实验结果表明肝素固定后的PLA膜具有良好的血液相容性。(2)天然水蛭素可以和PVP形成氢键。通过氢键相互作用将水蛭素分子固定在聚乳酸膜表面,从而制备出一种新型的抗凝聚乳酸膜。凝血四项(APTT,PT,TT,FIB)测试结果表明水蛭素固定膜具有良好的血液相容性。透析模拟实验表明,新制备的水蛭素固定聚乳酸膜具有良好的尿素、肌酐、溶菌酶清除率和较高的BSA保留率。(3)通过自由基共聚合成类肝素预聚物P(SSNa-VTES-AAH),然后利用表面微溶胀及界面交联策略,将类肝素共聚物P(SSNa-VTES-AAH)固定在膜表面,从而提高聚乳酸膜的血液相容性。类肝素分子修饰的PLA膜表面血液相容性明显提高,血小板吸附量相对较少,并且具有良好的透析性能。
[Abstract]:Polylactic acid (PLA), as a biological polymer, has the advantages of good film-forming, controllable degradation, easy structure control and high added value. However, the surface antifouling and biocompatibility of PLA microporous membranes need to be further improved. In order to meet the potential applications in biomedical applications such as dialysis, PLA microporous membranes were prepared by non-solvent induced phase separation. Hydrophilic segments (such as PVP segment) were introduced into the membrane surface by micro-swelling and interfacial crosslinking to improve their hydrophilic and antifouling properties, and further surface modification of anticoagulant groups (such as heparin, heparin, heparin) was carried out to improve the hydrophilic and antifouling properties of the membranes. Hirudin and heparin can improve the blood compatibility of PLA microporous membrane. The main research contents are as follows: preparation and blood compatibility of PLA membrane fixed with heparin, preparation of hirudin hydrogen bond fixed membrane and anticoagulant study; The study on the blood compatibility of poly (lactic acid) microporous membrane modified by heparin. The physical and chemical morphology, hydrophilicity, blood compatibility and dialysis performance of the membrane were studied. Conclusion: (1) Heparin was immobilized on the surface of PLA membrane by amidation reaction and adsorption, which improved the blood compatibility of PLA membrane surface. Firstly, Hep-APTES compounds were synthesized by amidation reaction. Then Hep-APTES was immobilized on the surface of PLA film by self-condensation reaction and multilayer adsorption. Heparinized modified PLA membrane was prepared. The experimental results showed that the heparin immobilized PLA membrane had good blood compatibility. 2) Natural hirudin could form hydrogen bond with PVP. Hirudin molecules were immobilized on the surface of polylactic acid membrane by hydrogen bond interaction. Thus a new anticoagulant polylactic acid membrane was prepared. The test results showed that hirudin fixed membrane had good blood compatibility, and the dialysis simulation experiment showed that the newly prepared hirudin immobilized PLA membrane had good urea. Creatinine, lysozyme clearance and high retention rate of BSA were synthesized by free radical copolymerization of heparin-like prepolymer (Pna-VTES-AAHN), and then the heparin-like copolymer PSSNa-VTES-AAHH was immobilized on the membrane surface by surface microswelling and interfacial crosslinking strategy. Therefore, the blood compatibility of polylactic acid membrane was improved, the surface of PLA membrane modified by heparin was improved obviously, the platelet adsorption was relatively small, and the hemodialysis performance was good.
【学位授予单位】：青岛科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ317;R318.08

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