不同孕期应激对成年子代大鼠情绪及认知功能的影响

发布时间：2018-03-02 11:43

本文选题：母孕期应激　切入点：单一延长应激　出处：《河北医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察不同孕期经历应激对成年子代大鼠认知及情绪功能的影响。方法:将16只健康清洁级Sprague Dawley(SD)雌性大鼠随机分成4组:对照组(CON)、孕早期组(PS1,妊娠第1-7d)、孕晚期组(PS3,妊娠第15-21d)、孕早期组+孕晚期组(PS1-3,妊娠第1-7d联合15-21d),每组各4只。PS1、PS3、PS1-3组分别在妊娠第7d、第15d、第7d和第15 d给予单一延长刺激(single-prolonged stress,SPS),对照组在妊娠期不给予任何处理。第8w末采用蔗糖偏好、强迫游泳、高架十字迷宫及Morris水迷宫试验分别评价其抑郁、焦虑和认知功能。实验数据采用SPSS21.0进行统计学分析。蔗糖偏好率(G,%)、强迫游泳后4分钟不动时间(T4,单位:s)、高架十字迷宫中进入开臂的次数及时间(s)、水迷宫前5天平均逃避潜伏期(s)、第6天的穿越平台象限时间(s)及次数数据以均数±标准差(sx?)表示。检验水准为0.05。对蔗糖偏好率、强迫游泳后4分钟静止不动时间(T4,单位:s)、高架十字迷宫中进入开臂的次数及时间(s)进行单因素方差分析和Bonferroni法两两比较,水迷宫前5天大鼠的平均逃避潜伏期进行重复测量分析。水迷宫第6天大鼠穿越平台象限时间(单位:s)及次数采用进行单因素方差分析和Bonferroni法两两比较。结果:1在蔗糖偏爱试验中,不同孕期应激组(F=52.597,P=0.000)及性别(F=5.931,P=0.018)均有统计学差异。两两比较的结果显示,PS3的蔗糖偏爱率比CON组低而比PS1高。同性别之间比较,雌性子代:PS3的蔗糖偏爱率比CON组低而比PS1高,差异有统计学意义(P0.01);雄性子代:PS3的蔗糖偏爱率比CON组低而比PS1高,差异有统计学意义(P0.01)。性别之间比较:PS1、PS1-3及CON雌雄之间均无差异(P0.01),PS3组雌性蔗糖偏爱率比PS3组雄性低,且差异有统计学意义(P0.01)。PS1-3组与对照组无统计学意义(P0.01)。2比较强迫游泳后4min的不动时间,不同孕期应激组(f=17.287,p=0.000)及性别(f=4.745,p=0.033)均有统计学差异。两两比较的结果显示,ps3在后4min的不动时间高于con而ps1及ps1-3低于con。同性别之间比较,雌性子代:ps3在后4min的不动时间高于con,而ps1及ps1-3低于con,差异有统计学意义(p0.01);雄性子代:ps3与con相比在后4min的不动时间增加而ps1及ps1-3降低,差异有统计学意义(p0.01)。性别之间比较:ps1、ps1-3及con雌雄之间均无差异(p0.01),ps3组雌性后4min的不动时间比ps3组雄性增加,且差异有统计学意义(p0.01)。3在高架十字迷宫实验中,于开臂中停留的时间中,不同孕期应激组(f=5.326,p=0.002)及性别(f=11.638,p=0.001)均有统计学差异。两两比较的结果显示,孕期应激三组均比con减少且差异有统计学意义(p0.01),而孕期应激三组之间相比无统计学意义(p0.01)。孕晚期应激组的雌性与雄性相比减少且差异有统计学意义(p0.01)。在进入开臂的次数中,不同孕期应激组(f=13.216,p=0.000)有统计学差异而性别(f=1.126,p=0.293)无统计学差异,ps1及ps3组较con进入开臂的次数减少有统计学差异(p0.05)。4水迷宫测试中,在前5天,不同孕期应激组(f=10.653p=0.000)及时间(f=82.068,p=0.000)均有统计学差异。但是性别之间没有统计学差异(f=0.000,p=0.100)。多变量方差分析显示:水迷宫第1d,在逃避潜伏期上,ps3和ps1-3有轻微的减少而ps1有轻微的增加,但是均没有统计学意义(f=1.963,p0.05),水迷宫2~5天,各组之间el差异均有统计学意义(f分别为5.057,4.463,11.052,10.245,p0.05),其中第2天,ps3及ps1-3的el低于对照组;第3天,仅ps1-3组el低于对照组;第4天、5天,ps1的el高于对照组,差异有统计学意义(p0.01)。第6天,经单因素方差分析,结果显示四组间穿越平台象限的时间及次数均有统计学意义(f分别为6.801,9.014,p0.01),但是性别之间没有统计学意义(p分别为0.173,0.036,f0.05)。两两比较bonferroni分析显示,在穿越平台象限时间及次数中,ps3组均高于对照组(p0.05)。结论:不同孕期应激对子代成年后情绪及认知功能影响不同。1孕早期经历应激能够降低抑郁样行为的出现,引起焦虑行为的产生,损害后代学习能力。2孕晚期经历应激能够导致抑郁样行为的出现,引起焦虑行为的产生,促进后代学习记忆能力。3孕早期联合孕晚期经历应激对成年后代的情绪及认知功能影响不大。4性别之间,只有孕晚期经历应激的成年雌性后代更易出现焦虑抑郁样行为。
[Abstract]:Objective: To observe the different effects of stress on pregnancy experience in adult offspring rats cognitive and emotional function. Methods: 16 healthy Sprague Dawley (SD) female rats were randomly divided into 4 groups: control group (CON), early pregnancy group (PS1, 1-7d of pregnancy, late pregnancy group (PS3). 15-21d of pregnancy), early pregnancy group and late pregnancy group (PS1-3, 1-7d of pregnancy combined with 15-21d), 4 rats in each group.PS1, PS3, PS1-3 group respectively in the 7d of pregnancy, 15d, 7d and d give fifteenth single (single-prolonged stress, SPS prolonged stimulation), the control group not given during pregnancy any treatment. At the end of 8W by sucrose preference, forced swimming, elevated plus maze and Morris water maze test were evaluated for depression, anxiety and cognitive function. The experimental data were statistically analyzed by SPSS21.0. The sucrose preference rate (G,%), 4 minutes after the forced swimming immobility time (T4, unit: s). The elevated plus maze into the open The number of arm water maze (s), 5 days before the average escape latency (s), sixth days (s) through the platform quadrant time and frequency data to mean + standard deviation (SX?). The test level of 0.05. on the rate of sucrose preference, forced swimming for 4 minutes after the immobility time (T4: s), elevated plus maze in the open arm of the times and time (s) were compared with single factor variance analysis and Bonferroni method 22, 5 days before the water maze rats the average escape latency for repeated measurement analysis. Water maze rats sixth days through the platform quadrant time (unit: S) and the number of times were compared using one-way ANOVA and Bonferroni method. Results: 1 in 22 sucrose preference test in different pregnancy stress group (F=52.597, P=0.000) and gender (F=5.931, P=0.018) were statistically significant difference. 22 comparison results show that PS3 sucrose preference rate lower than CON group and higher than PS1. The same Comparison between the sexes, female offspring: PS3 sucrose preference rate lower than CON group and higher than PS1, the difference was statistically significant (P0.01); male offspring: PS3 sucrose preference rate lower than CON group and higher than PS1, the difference was statistically significant (P0.01). The comparison between gender: PS1, PS1-3 and there were no differences in CON and PS3 group (P0.01), sucrose preference rate of PS3 group than in female male is low, and the difference was statistically significant (P0.01) in.PS1-3 group and control group had no statistical significance (P0.01).2 4min forced swimming immobility time in different pregnancy stress group (f= 17.287, p=0.000) and gender (f=4.745, p=0.033) were statistically significant difference. 22 comparison results show that PS3 in 4min after the real time higher than con and PS1 and ps1-3 lower than the comparison between con. and sex, female offspring: PS3 in 4min after the real time higher than that of con, PS1 and ps1-3 is lower than con, the difference was statistically significant (P0.01 male); Offspring: PS3 compared with con in 4min after the real time increased while PS1 and ps1-3 decreased, the difference was statistically significant (P0.01). The gender comparison between PS1, ps1-3 and con between male and female were no difference (P0.01), PS3 group of female 4min after the immobility time in PS3 group than in the male, and the poor there was a significant correlation (P0.01).3 in the elevated plus maze, to open arms stay in different pregnancy stress group (f=5.326, p=0.002) and gender (f=11.638, p=0.001) were statistically significant difference. 22 comparison results show that prenatal stress in three groups were less than in con and the difference was statistically significant (P0.01), and between prenatal stress compared to the three groups had no statistical significance (P0.01). The male and female decreased and the difference was statistically significant compared with late pregnancy stress group (P0.01). The number of open arm in different pregnancy stress group (f =13.216, p=0.000) and gender difference (f=1 .126, p=0.293) had no significant difference, PS1 and PS3 compared with group con in open arms reductions in the number of statistically significant (P0.05).4 water maze test, 5 days ago, during different stress group (f=10.653p=0.000) and time (f=82.068, p=0.000) were statistically significant. But there were no statistical differences between genders (f=0.000, p=0.100). Multivariate analysis of variance showed that the 1D water maze test, the escape latency in PS3, and ps1-3 and PS1 have a slight increase decrease slightly, but there was no statistically significant difference (f=1.963, P0.05), 2~5 water maze, there were statistically significant differences between groups el (F = 5.057,4.463,11.052,10.245, P0.05 in second days, PS3), and ps1-3 El lower than the control group; the third day, only ps1-3 group El was lower than the control group; fourth days, 5 days, PS1 El was higher than the control group, the difference was statistically significant (P0.01). The sixth day, by the single factor analysis of variance, the results showed that the four groups Cross platform quadrant time and frequency were statistically significant (F = 6.801,9.014, P0.01), but there was no statistical significance between the sexes (P = 0.173,0.036, f0.05). 22 Bonferroni analysis showed that, in the time and frequency of passing through the platform quadrant in PS3 group were higher than control group (P0.05). Conclusion: different pregnancy stress in offspring in adulthood affect mood and cognitive function of different.1 in early pregnancy experience stress can reduce the anxiety caused by depression like behavior, behavior, damage to the offspring of the learning ability of.2 in late pregnancy experience can lead to stress depression like behavior, anxiety behavior, promote the ability of learning and memory of.3 offspring in early pregnancy and pregnancy advanced experience stress on adult offspring emotion and cognitive function has little effect on.4 sex between adult female offspring than in late pregnancy experience stress more prone to anxiety and depression Like behavior.

【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749

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