利用Janus纳米颗粒传感器检测肿瘤标记物PSA和基于GAV-9的多肽自组装的初探

发布时间：2018-03-05 19:21

本文选题：Janus　切入点：纳米颗粒　出处：《内蒙古农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：可控的材料分布对于构建各向异性的结构至关重要,同时它也可以作为一种设计参数。最近,Janus颗粒由于他的各向异性在研究和技术领域的很多应用中已经获得了很大的关注。本实验建立了一种一步两相的乳化合成方法,这种方法可广泛应用于合成具有多功能性的(例如表面等离子,磁性和荧光)Janus纳米颗粒。本实验利用Janus纳米颗粒的磁性和荧光特性,使Janus纳米颗粒分别的取代固定在微量滴定板上最先捕获的一抗和比色检测的酶联二抗。相对于传统的酶联免疫吸附实验,该法进行体外检测前列腺特异性抗原(PSA)更加简便,并且其线性浓度检测范围符合当前的临床诊断需要。我们希望这个大规模生产多功能性的Janus纳米颗粒的方法能应用于传统的比色生物传感器和现代精密医学这些领域。近几年,淀粉样多肽表面辅助的自组装在淀粉样变性研究和纳米技术中备受关注。研究淀粉样蛋白的纤维化过程以及造成蛋白纤维化的核心肽段序列,对于更好的理解这类疾病的致病机制以及探索治愈途径有重要意义。GAV-9(VGGAVVAGV)是α-synuclein、Aβ肽和prion是几个神经性退行性疾病相关蛋白一段保守序列。本实验通过研究基于GAV-9合成的GAV-12(NH2-VGGAWAGVVGG-CONH2)和G AV-15(NH2-VGGAVVAGVVGGAVV-CONH2)在云母表面的自组装过程,通过原子力显微镜的观察发现,它们与GAV-9类似,在云母表面以一种外延生长模式进行自组装。而且GAV-12和GAV-15的高度在G-AV-9高度的基础上分别增加了 0.7nm和1.4nm。
[Abstract]:Controlled distribution of materials is essential to the construction of anisotropic structures. It can also be used as a design parameter. Recently, the Janus particle has received great attention in many research and technical applications due to its anisotropy. A one-step two-phase emulsification synthesis method has been developed in this experiment. This method can be widely used in the synthesis of multifunctional (such as surface plasma, magnetic and fluorescent Janus nanoparticles). Janus nanoparticles were used to replace the first antibody which was first captured on the microtitration plate and the enzyme linked second antibody which was detected by colorimetry. Compared with the traditional enzyme-linked immunosorbent assay (Elisa), this method was more convenient for the detection of prostate specific antigen (PSA) in vitro. And its linear concentration detection range is in line with current clinical diagnostic needs. We hope this method of mass production of multifunctional Janus nanoparticles can be applied to traditional colorimetric biosensors and modern precision medicine. Surface assisted self-assembly of amyloid peptides has attracted much attention in amyloidosis research and nanotechnology. The process of amyloid protein fibrosis and the core peptide sequence that cause protein fibrosis are studied. GAV-9 VGGAVAGV is a conserved sequence of 伪 -synuclein A 尾 peptide and several neurodegenerative disease-associated proteins. Self-assembly process of GAV-12 (NH2-VGGAWAGVVGG-CONH2) and G-AV-15 (NH2-VGGAVAGVGGAVV-CONH2) on the surface of mica. It is found by atomic force microscopy that they are self-assembled on the surface of mica by an epitaxial growth pattern similar to that of GAV-9, and the heights of GAV-12 and GAV-15 are increased by 0.7nm and 1.4nm, respectively, on the basis of G-AV-9 height.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R730.43

【相似文献】