藏医名方联用对脑缺血损伤及行为学障碍的药效学研究

发布时间：2018-03-08 23:18

本文选题：二十五味珊瑚丸　切入点：如意珍宝丸　出处：《西南交通大学》2017年硕士论文　论文类型：学位论文

【摘要】：研究发现,民族药物在针对脑卒中和相关后遗症的临床治疗方面依然保持有高涨之势,其中以藏药最为突出。在经典藏医医著中记载到,对脑卒中病症都以血、脉、隆三者结合的方法,达到标本同治的目的。综合来看,藏医积累的经验及其独特的时辰给药理论都是治疗脑缺血类疾病的优势所在。本课题基于以上特点探索三种藏医名方二十五味珊瑚丸、如意珍宝丸、二十味沉香丸于早、中、晚联用对脑缺血损伤及行为学障碍的影响。1.建立MCAO/R模型,脑组织Nissl染色,观察神经元Nissl小体变化表明:藏医名方联用可有效减轻脑缺血受损引起的Nissl小体损伤,维护神经元结构完整性。采用IHC/S-P法和RT-PCR定量结果表明:藏医名方联用能有效促进Bcl-2蛋白含量,下调Caspase-3、NF-κBp65、ICAM-1、VEGF、TLR4蛋白表达,对脑缺血损伤引起的炎症反应及细胞凋亡有较好的改善作用。2.建立MCAO/R模型,血液流变学指标检测结果表明:藏医名方联用可显著降低缺血性脑卒中血管内全血粘度,血浆粘度,ESR,血沉方程K值,Hct,EAI,红细胞刚性指数;显著升高电泳指数,红细胞变形指数,改善血液流动力学特性;建立小鼠急性血瘀模型,耳廓微循环检测表明:藏医名方联用显著扩张肾上腺素所致急性血瘀模型小鼠耳廓微动脉、微静脉血管管径,加快血液流速,改善微循环。结合MCAO/R模型和小鼠急性血於模型,共同探讨三种藏医名方活血化瘀、扩张血管、促进血液流动及对血管功能的保护。3.建立小鼠记忆获得障碍模型,利用小鼠趋暗习性对其学习、记忆、反应能力测试结果表明:藏医名联用显著增加小鼠潜伏期、主动穿梭次数,且测试成绩优于训练成绩,保护M受体抑制剂所致小鼠脑损伤,减轻小鼠产生的学习记忆反应障碍的症状;有效降低小鼠脑组织中AChE、MDA活性,显著升高SOD含量,维护胆碱能神经功能,清除体内自由基,降低脂质过氧化损伤。
[Abstract]:The study found that there is still an upsurge in the clinical treatment of stroke and related sequelae, especially Tibetan medicine. In classical Tibetan medicine, it is recorded that blood and veins are used to treat stroke. The method of combination of the three Longs, to achieve the purpose of the same treatment of specimens. In a comprehensive view, The accumulated experience of Tibetan medicine and its unique theory of time administration are the advantages of treating cerebral ischemia diseases. Based on the above characteristics, this paper explores three kinds of famous Tibetan medicine prescription 25 coral pills, Ruyi Zhenbao pills, 20 kinds of Shenxiang pills in the early and middle. The effects of late combined use on cerebral ischemia injury and behavioral disorders. 1. MCAO/R model was established, brain tissue Nissl staining and changes of neuron Nissl corpuscles were observed. The results showed that Tibetan medicine combined with famous prescription could effectively reduce Nissl corpuscle injury caused by cerebral ischemia injury. The results of IHC/S-P and RT-PCR quantitative analysis showed that the combination of Tibetan medicine and famous prescriptions could effectively promote the content of Bcl-2 protein and down-regulate the expression of Caspase-3, NF- 魏 Bp65, ICAM-1 and VEGFTLR4 protein. The model of MCAO/R was established. The results of hemorheological index test showed that the combination of Tibetan medicine and famous prescription could significantly reduce the whole blood viscosity of blood vessel in ischemic stroke. Plasma viscosity, ESR, erythrocyte rigidity index, erythrocyte rigidity index, erythrocyte deformability index, erythrocyte deformability index, erythrocyte deformability index, and establishment of acute blood stasis model in mice. The detection of auricle microcirculation showed that the acute blood stasis model of mice induced by epinephrine was significantly expanded by the famous prescription of Tibetan medicine, which could accelerate the blood flow rate and improve the microcirculation, combining with the MCAO/R model and the acute blood flow model of mice, the diameter of the arterioles and venules of the auricle of the mice was increased. To explore three famous Tibetan medicine prescriptions, such as activating blood circulation and removing blood stasis, dilating blood vessels, promoting blood flow and protecting vascular function .3.To establish a model of memory acquisition disorder in mice, and to learn and remember it by using dark habit of mice. The results of response ability test showed that the combination of Tibetan medicine and Chinese medicine could significantly increase the latency and the number of active shuttles in mice, and the test scores were better than the training results, which protected mice from brain injury induced by M receptor inhibitors. The symptoms of learning and memory reaction in mice were alleviated, the activity of ache MDA in brain tissue was decreased, the content of SOD was increased significantly, cholinergic nerve function was maintained, free radical was cleared and lipid peroxidation injury was reduced.
【学位授予单位】：西南交通大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R29

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