甘露清凉饮对金黄地鼠颊癌的抑瘤作用及其机制初探

发布时间：2018-03-17 18:43

本文选题：金黄地鼠　切入点：颊癌　出处：《河北医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)是目前较多见的破坏性最大的癌症之一,恶性程度高,位于口腔颌面部,手术治疗是目前最常用的治疗方法,但因此会造成患者面容缺损,功能障碍,严重影响其生活。虽然配合其他疗法,患者的5年生存率仍停留在50%左右,且局部复发率极高。因此我们迫切渴望寻找到综合有效的方法来保存患者组织器官的完整性,提高其生活质量。现在有越来越多的中药应用到恶性肿瘤的治疗当中,都取得了不错的效果,而且这种趋势有增无减。本实验采用中药甘露清凉饮对建立的实验动物模型口腔颊黏膜鳞癌进行治疗,观察其是否可以抑制口腔癌的发生及发展,并对其抗肿瘤机制进行初步探讨,为今后的实验和临床应用提供实验理论依据。方法:实验对象为85只6~8周龄、体重约100 g的叙利亚金黄地鼠,雌雄兼备,分笼喂养,取10只为空白对照组,不作任何处理;其余75只金黄地鼠用0.3%DMBA注射右侧颊囊12周,第13周时将成瘤的60只实验动物采用随机数字表法将地鼠分为3个用药组和1个模型对照组(每组15只),低剂量甘露清凉饮组、中剂量甘露清凉饮组及高剂量甘露清凉饮组分别灌服7.5、15、30 g/kg的甘露清凉饮4周,空白对照组不做任何处理。第17周处死全部动物,记录肉眼肿瘤发生率、肿瘤数目及体积,肿瘤组织行HE染色镜下观察,用免疫组化方法检测组织中BCL-2、CD86的表达。所有数据采用SPSS 13.0统计软件,P0.05具有统计学意义。结果:1肉眼可见肿瘤发生率模型对照组为91.5%,低剂量甘露清凉饮组81.4%,中剂量甘露清凉饮组76.9%,高剂量甘露清凉饮组69.6%(P0.01);各用药组平均肿瘤数目均较模型对照组2.43±1.55个有所降低,其中中剂量甘露清凉饮组1.47±1.04个和高剂量甘露清凉饮组1.16±1.02个显著下降(P0.01)。2各用药组组织病理学观察鳞状细胞癌病变数目均较模型对照显著降低(P0.05)。3各用药组与模型对照组相比均能降低病损处的血管密度,尤以中、高剂量甘露清凉饮组效果更佳(P0.05)。4各用药组免疫组化检测BCL-2较模型对照组表达水平有所降低,高剂量组甘露清凉饮效果最为显著(P0.05)。5各用药组免疫组化检测CD86均呈高表达,高于模型对照组,尤以高剂量甘露清凉饮效果更佳(P0.05)。结论:1甘露清凉饮可抑制金黄地鼠颊粘膜鳞癌的生长,其中高剂量给药方式抑癌作用效果最好。2甘露清凉饮可以通过减少血管新生抑制金黄地鼠颊癌的发生发展。3甘露清凉饮可能是通过下调BCL-2的表达来促进颊癌细胞的凋亡。4上调免疫细胞CD86的表达,提高肿瘤监视功能,可能是甘露清凉饮抑癌作用的机制之一。
[Abstract]:Objective: oral squamous cell carcinoma (OSCC) is one of the most destructive cancers in oral squamous cell carcinoma. It is located in oral and maxillofacial region with a high degree of malignancy. Surgical treatment is the most commonly used method at present, but it can cause facial defects in patients. The 5-year survival rate of the patients remained at around 50%, despite the combination of other therapies. And the local recurrence rate is extremely high. Therefore, we are eager to find comprehensive and effective methods to preserve the integrity of patients' tissues and organs and improve their quality of life. Now more and more Chinese medicines are used in the treatment of malignant tumors. This experiment used mannoxingliangyin to treat oral and buccal squamous cell carcinoma, and observed whether it could inhibit the occurrence and development of oral cavity cancer. The mechanism of anti-tumor was preliminarily discussed in order to provide experimental theoretical basis for the future experiment and clinical application. Methods: 85 Syrian golden hamsters, weighing about 100g, aged 6 weeks and 8 weeks old, were fed in separate cages, both male and female. The other 75 golden hamsters were injected with 0.3DMBA for 12 weeks. At the 13th week, 60 experimental animals were randomly divided into 3 drug groups and 1 model control group (15 rats in each group, low dose mannu Qingliang decoction group). The middle dose Ganlu Qingliang decoction group and the high dose Ganlu Qingliang decoction Group were given 7.5U 1530g / kg Mannu Qingliang drink for 4 weeks respectively, and the blank control group did not do any treatment. At the 17th week, all the animals were killed and the incidence, number and volume of the tumor were recorded. The tumor tissue was observed by HE staining. The expression of BCL-2 + CD86 in tissues was detected by immunohistochemical method. All the data were statistically significant by SPSS 13.0 statistical software (P0.05). Results the incidence rate of tumor was 91.5 in the model control group and 81.4 in the low-dose Gannuqingliang Yin group, and the middle dose was found in the low dose of Gannuqingliang Yin group. The average number of tumors in the Ganlu Qingliang decoction group was lower than that in the model control group (2.43 卤1.55), and that in the mannoxiangliang drink group (69.6) was lower than that in the model control group (P 0.01). Among them, 1.47 卤1.04 in medium dose Ganlu Qingliang decoction group and 1.16 卤1.02 in high dose Ganlu Qingliang decoction group were significantly decreased P0.01T.2 the number of squamous cell carcinoma lesions in each drug group was significantly lower than that in model control group and model control group (P 0.05.3). Compared with the control group, the blood vessel density in the lesion was decreased. In particular, the expression of BCL-2 in the high dose group was lower than that in the model control group. The expression of CD86 was significantly higher in the high dose group than that in the model control group. The expression of CD86 in each drug group was significantly higher than that in the high dose group (P0.055.The expression of CD86 in each group was higher than that in the control group). Compared with the model control group, the effect of high dose manlu Qingliang drink was better than that of the model control group (P 0.05). Conclusion the growth of buccal mucosa squamous cell carcinoma of golden hamster can be inhibited by W1 Ganlu Qingliang decoction. The effect of high dose administration is the best. 2. Mannoxinqingliangyin can inhibit the occurrence and development of golden hamster buccal carcinoma by reducing angiogenesis. 3 mannose Qingliang drink may promote buccal cancer cells by down-regulating the expression of BCL-2. Upregulate the expression of CD86 in immune cells, Enhancing tumor surveillance may be one of the mechanisms of manlu Qingliang Yin's anti-cancer effect.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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