瘦素受体基因多态性与乳腺癌风险相关性的系统评价与Meta分析

发布时间：2018-04-01 03:22

本文选题：瘦素受体　切入点：乳腺癌　出处：《江苏大学》2017年硕士论文

【摘要】：目的:许多流行病学研究已经发现瘦素与身体脂肪程度以及乳腺癌有关。瘦素通过瘦素受体(LEPR)发挥其生理作用受体。但是,目前已发表的关于LEPR与乳腺癌发生之间关系的数据仍充满争议。本研究采用系统评价方法分析已发表的相关文献的相关数据,来得出更准确的评估LEPR多态性与乳腺癌罹患风险关系的结论,为乳腺癌的诊治提供一定的指导。方法:本研究按照设定的关键词检索了Pub Med Central、ISI知识网、CNKI以及Embase数据库等数据库中从建库至2012年04月20日发表的临床研究。对符合纳入标准的研究进行资料提取后用Stata 10.0软件进行数据分析。对LEPR多态性与乳腺癌罹患风险关联强度采用比值比(OR)及其95%可信区间(CI)描述。各研究间的异质性采用Q检验及I2检验评价。当各研究结果间异质性较低时,采用固定效应模型进行分析;否则则采用随机效应模型进行分析。异质性分析采用亚组分析及敏感性分析。发表偏倚采用Begg漏斗图以及Egger’s线性回归分析,P0.05为差异有统计学意义。结果:本Meta分析共纳入10项LEPR rs1137101多态性研究,包括4644例病例组和5485例对照组的;共纳入5项rs1137100多态性研究包括2759例病例组和4464例对照组以及纳入2项rs8179183、rs4655537和rs3762274多态性研究。采用合并的比值比(OR)与95%置信区间(CI)估计乳腺癌风险与LEPR基因型的相关性。当所有研究合并后发现升高的乳腺癌风险与LEPR相关rs1137101多态性有关,(等位基因对比模型:OR=0.71,95%CI=0.551-0.997)。在按种族分层分析后发现,等位基因对比模型(OR=0.414,95%CI=0.312-0.550)以及显性模型(OR=0.537,95%CI=0.370-0.781)中亚洲人乳腺癌发生风险显著增加。对于非洲人,等位基因对比模型(OR=0.716,95%CI=0.595-0.861)、纯合子显性模型(OR=0.537,95%CI=0.370-0.781)和显性模型(OR=1.595,95%CI=1.207-2.108)中也发现乳腺癌发生风险显着增加。并且LEPR rs1137100多态性的等位基因对比模型(OR=0.666,95%CI=0.603-0.720)和纯合子共显性模型(OR=0.344,95%CI=0.282-0.421)中乳腺癌发生风险是增加的。对于LEPRrs8179183、rs4655537和rs3762274多态性,所有比较模型中都没发现与乳腺癌发生风险相关。结论:本研究发现rs1137101和rs1137100多态性与乳腺癌发生风险显着相关,LEPR rs1137101变体的A等位基因和LEPR rs1137100变体的G等位基因是发生乳腺癌的低危因素。此外,LEPR rs8179183、rs4655537和rs3762274多态性和乳腺癌发生的风险并没有显著的联系。
[Abstract]:Objective: many epidemiological studies have found that leptin is associated with body fat levels and breast cancer. Leptin plays its physiological role through leptin receptor Lepr. The published data on the relationship between LEPR and breast cancer is still controversial. To arrive at a more accurate assessment of the association between LEPR polymorphism and breast cancer risk. Methods: in this study, we searched the database of Pub Med Central Pub and Embase database from the establishment of the database to 20 April, 2012, according to the key words set up in the diagnosis and treatment of breast cancer. The data were extracted and analyzed by Stata 10.0 software. The ratio of LEPR polymorphism to breast cancer risk was described by odds ratio (OR) and 95% confidence interval (CI). The heterogeneity of each study was analyzed. Q test and I2 test were used to evaluate. When the heterogeneity between the results of each study was low, The fixed effect model is used to analyze; Otherwise, random effect model was used. Heterogeneity analysis was based on subgroup analysis and sensitivity analysis. Publication bias was analyzed by Begg funnel graph and Egger's linear regression analysis (P0.05). Results: this Meta score was statistically significant. Ten LEPR rs1137101 polymorphisms were studied. Including 4644 cases and 5485 cases of control group; A total of 5 rs1137100 polymorphism studies were included, including 2759 cases and 4464 controls, as well as 2 rs8179183 rs4655537 and rs3762274 polymorphism studies. The association between breast cancer risk and LEPR genotype was estimated by using combined odds ratio (ORR) and 95% confidence interval (CI). Sex. When all studies were combined, increased breast cancer risk was found to be associated with LEPR associated rs1137101 polymorphisms (allelic comparison model: OR0.71, 95 CIQ 0.551-0.997). There was a significant increase in the risk of breast cancer in Asians in the allelic control model ORA 0.41495 CI 0.312-0.550) and the dominant model ORA 0.53795 CI0.370-0.781. for Africans, there was a significant increase in the risk of breast cancer. A significant increase in the risk of breast cancer was also found in OR1.595-0.861CII, OR0.53795 CIQ 0.370-0.781) and the dominant model OR1.595-95CI1.207-2.108. and the LEPR rs1137100 polymorphism allelic correlation model OR0.66695CIT 0.603-0.720) and the homozygous codominant model OR0.34495CIQ 0.282-0.421) were also found to have a significant increase in the risk of breast cancer. The risk of occurrence is increased. For LEPRrs8179183 rs4655537 and rs3762274 polymorphism, Conclusion: rs1137101 and rs1137100 polymorphisms were significantly associated with breast cancer risk by allele A of LEPR rs1137101 variant and G allele of LEPR rs1137100 variant were found in all comparative models. Conclusion: in this study, we found that the polymorphism of rs1137101 and rs1137100 was significantly associated with the risk of breast cancer and the G allele of the variant of LEPR rs1137100 was significantly associated with the risk of breast cancer. In addition, LEPR rs8179183 rs4655537 and rs3762274 polymorphism were not significantly associated with the risk of breast cancer.
【学位授予单位】：江苏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.9

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