一枝蒿挥发油对过敏性鼻炎的作用研究

发布时间：2018-04-04 00:19

本文选题：一枝蒿药材　切入点：一枝蒿挥发油　出处：《新疆医科大学》2017年硕士论文

【摘要】：目的:(1)优选一枝蒿挥发油提取工艺。(2)分析不同产地一枝蒿挥发油的化学成分,对其种类及含量进行对比研究,优选一枝蒿药材。(3)优选一枝蒿挥发油液体制备工艺。(4)分别建立小鼠耳肿胀模型、小鼠腹腔毛细血管通透性模型研究一枝蒿挥发油抑制炎症作用。(5)建立小鼠迟发型超敏反应模型,探讨一枝蒿挥发油的抗过敏作用。(6)建立过敏性鼻炎(AR)大鼠模型,探讨一枝蒿挥发油对过敏性大鼠模型的治疗作用。方法:(1)通过单因素实验,以出油率为指标,优选一枝蒿挥发油提取工艺。(2)利用水蒸气蒸馏法提取一枝蒿挥发油,用归一化法测定相对含量,GC-MS法对三个不同产地一枝蒿挥发油的化学成分进行鉴定、分析。(3)以一枝蒿挥发油的增溶量为指标,优选一枝蒿挥发油液体的处方。(4)建立二甲苯致小鼠耳肿胀、冰醋酸致小鼠腹腔毛细血管通透性实验的动物模型,观察一枝蒿挥发油的炎症抑制作用。(5)采用2,4-二硝基氯苯建立小鼠迟发型超敏反应模型,探讨一枝蒿挥发油抗过敏的作用。(6)利用卵清蛋白(OVA)氢氧化铝混悬液进行全身致敏和鼻部抗原攻击,建立过敏性鼻炎大鼠模型。采用酶联免疫吸附(ELISA)法,测定大鼠血清中免疫球蛋白E(IgE)、特异性IgE(s IgE)及白介素-4(IL-4)的含量,并取鼻粘膜做病理组织学检查,探讨一枝蒿挥发油对大鼠血清IgE、sIgE、IL-4以及鼻粘膜组织结构、肥大细胞、炎性细胞浸润等情况的影响。结果:(1)药材粗粉,以1:8的料液比,浸润5h,回流提取5h,为一枝蒿挥发油最佳提取工艺。(2)三个不同产地一枝蒿挥发油的主要化合物及其含量均有差异,并首次分析出十几种化合物。(3)一枝蒿挥发油液体制备优选工艺为表面活性剂洛沙姆188,与一枝蒿挥发油比例为56:1,处方量的其余辅料混合可制得外观稳定的一枝蒿挥发油液体溶液。(4)一枝蒿挥发油给药组二甲苯致小鼠耳肿胀程度与模型组相比较轻,差异有显著性(P0.05)。(5)一枝蒿挥发油高剂量组与模型组比较,小鼠腹腔毛细血管通透性降低,差异有显著性(P0.05)。(6)一枝蒿挥发油给药组大鼠较模型组,大鼠鼻部病症、鼻粘膜病变以及大鼠血清IgE、s Ig E及IL-4含量与模型组相比有所减少,有显著性差异(P0.05)。结论:(1)提取工艺研究结果可为后续研究提供理论依据。(2)在理论上初步阐释了同一采摘时间不同产地一枝蒿挥发油化学成分种类及含量的不同之处,对其药效学研究有参考价值。(3)研究制得的一枝蒿挥发油液体性质均一、稳定。(4)一枝蒿挥发油对过敏性鼻炎有明显的治疗作用。(5)一枝蒿挥发油能抑制过敏性鼻炎大鼠IL-4的分泌,减少过敏性鼻炎大鼠血清IgE、s IgE含量,并缓解鼻粘膜的病变。(6)一枝蒿挥发油对过敏性鼻炎大鼠有一定的医治效果。
[Abstract]:Objective to analyze the chemical constituents of volatile oil from Artemisia annua from different habitats, and to compare its species and contents.Preparation technology of volatile oil of Artemisia annua L.) the mouse ear swelling model was established, and the mouse model of celiac capillary permeability was established. The model of delayed type hypersensitivity reaction was established by the inhibition of inflammation by the volatile oil of Artemisia annua L.To study the antiallergic effect of essential oil of Artemisia annua L.) to establish the rat model of allergic rhinitis and to explore the therapeutic effect of volatile oil of Artemisia annua on allergic rat model.Methods (1) A single factor experiment was conducted to optimize the extraction process of the volatile oil from Artemisia annua by using the oil yield as the index. The volatile oil of Artemisia annua was extracted by steam distillation.The chemical constituents of the volatile oil of Artemisia annua from three different habitats were identified by GC-MS, and the solubilization of volatile oil of Artemisia annua was used as the index.To establish an animal model of mice ear swelling induced by xylene and capillaries permeability induced by acetic acid in mice.To observe the inflammatory inhibitory effect of volatile oil of Artemisia annua L. 5) mice model of delayed type hypersensitivity was established by using 2o 4-dinitrochlorobenzene.To study the antiallergic effect of essential oil of Artemisia annua L. 6) to establish allergic rhinitis rat model by using ovalbumin OVA) aluminum hydroxide suspension for systemic sensitization and nasal antigen attack.The effects of mast cells and inflammatory cell infiltration.Results the crude powder was the best extraction technology of volatile oil of Artemisia mongolica for 5 h and refluxing for 5 h. The main compounds and contents of volatile oil of Artemisia annua from three different producing areas were different.It is the first time to analyze more than a dozen compounds. 3) the optimum preparation process of volatile oil liquid of Artemisia annua is as follows: surfactant Losham 188. the ratio of volatile oil of Artemisia annua to Artemisia annua is 56: 1. The stable appearance of Artemisia artemisia can be obtained by mixing the other excipients with the prescription amount.The ear swelling of mice induced by xylene in the volatile oil treatment group was lighter than that in the model group.Compared with the model group, the contents of IgE Ig E and IL-4 in nasal mucosal lesions and serum of rats were significantly lower than those in the model group (P 0.05).Conclusion (1) the results of extraction process can provide theoretical basis for further study. (2) the differences of chemical constituents and contents of volatile oil of Artemisia annua from the same picking time and different producing areas were preliminarily explained in theory.The essential oil of Artemisia annua L. is homogeneous in nature and stable. 4) the volatile oil of Artemisia annua has obvious therapeutic effect on allergic rhinitis. 5) the volatile oil of Artemisia annua can inhibit the secretion of IL-4 in allergic rhinitis rats.The essential oil of Artemisia annua has a certain therapeutic effect on allergic rhinitis rats.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284;R285.5

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