续骨丹对大鼠骨缺损模型血清BMP-2的影响

发布时间：2018-04-19 04:36

本文选题：续骨丹 + 骨缺损　；参考：《南京中医药大学》2017年硕士论文

【摘要】：目的:运用Elisa、HE染色、X光检测等技术,观察续骨丹外敷疗法对大鼠胫骨大块缺损模型血清骨形态发生蛋白-2(BMP-2)表达的影响,以及诱导骨缺损的成骨情况和最终骨修复的结果,初步揭示续骨丹促进骨缺损修复、康复的机理。方法:将70只SD大鼠右侧胫骨制备1个4mm缺损,用0.8mm克氏针行内固定。造模1个月后将符合标准的大鼠随机分为实验组(药物治疗组)、对照组a(脉冲磁场治疗组)、对照组b(面粉治疗组)和空白组。分别在干预后的0、2、4周,对大鼠右胫骨进行X线检查,然后处死动物,收获骨样本及血清,做苏木素-伊红(HE)染色和血清BMP-2酶联免疫检测。结果:①干预0周时,实验组、对照组a、对照组b和空白组大鼠血清BMP-2的含量没有明显差异;干预至第2周时,四组大鼠血清BMP-2的含量与干预前相比均下降,但组间差异均无统计学意义(P0.05);干预4周后,实验组大鼠血清BMP-2的含量高于对照组a,差异有统计学意义(P0.05),实验组大鼠血清BMP-2的含量高于对照组b和空白组,差异有统计学意义(P0.01),对照组a血清BMP-2含量明显高于对照组b和空白组,差异有统计学意义(P＜0.01),对照组b与空白组比较,无明显差异(p0.05)。三组大鼠血清BMP-2的含量在干预0、2、4周后的变化趋势是:实验组、对照组a先降后升,对照组b和空白组一直下降。②干预至第4周,四组大鼠骨样本HE染色结果显示:实验组大鼠中的4只和对照组a中的2只显示横断面上骨组织基本完整,骨髓组织丰富或比较丰富,少量骨组织与骨髓组织交错存在;空白组及对照组b大鼠染色均显示骨组织不同程度的残缺及骨髓组织稀少或欠丰富。③干预至第4周,四组大鼠骨缺损造模侧肢体X光片显示:实验组5只大鼠有4只基本愈合,对照组a中5只大鼠有2只基本愈合,愈合胫骨X片显示胫骨原缺损处骨皮质连续,未见骨折线存在;对照组b和空白组大鼠右胫骨均未完全愈合,X片显示胫骨中下段骨皮质不连,骨折线存在明显,周围未见明显骨痂生长。结论:续骨丹外敷疗法和脉冲电磁场疗法均能在干预至第4周时使处于下降趋势内源性BMP-2重新高表达,诱导骨修复,促进骨缺损的康复愈合。但续骨丹外敷疗法在对BMP-2的调节作用上明显优于脉冲电磁场疗法。
[Abstract]:Objective: to observe the effect of Xray on the expression of serum bone morphogenetic protein (BMP-2), bone morphogenetic protein-2 (BMP-2) in rats with large tibial defect, and to investigate the osteogenesis of bone defect and the result of bone repair.The mechanism of promoting bone defect repair and rehabilitation was revealed.Methods: one 4mm defect was prepared from the right tibia of 70 SD rats and was fixed with 0.8mm Kirschner needle.One month later, the rats who met the standard were randomly divided into experimental group (drug treatment group), control group (pulse magnetic field treatment group), control group B (flour treatment group) and blank group.The right tibia of the rats were examined by X-ray at 0 ~ 2 weeks after intervention, and then the animals were killed, bone samples and serum samples were harvested, hematoxylin-eosin) staining and serum BMP-2 enzyme-linked immunosorbent assay were performed.Results there was no significant difference in the content of serum BMP-2 between experimental group, control group, control group and blank group at 0 weeks after intervention by 1: 1, and by the second week of intervention, the content of serum BMP-2 in the four groups was lower than that before intervention.After 4 weeks of intervention, the content of serum BMP-2 in the experimental group was higher than that in the control group (P 0.05), and the content of serum BMP-2 in the experimental group was higher than that in the control group (b) and the blank group (P < 0.05).The level of serum BMP-2 in control group was significantly higher than that in control group and blank group (P < 0.01), but there was no significant difference between control group and blank group (P < 0.01).The change trend of serum BMP-2 content in the three groups after 4 weeks of intervention was as follows: in the experimental group, the control group a decreased first and then increased, the control group b and the blank group decreased 2. 2 until the 4th week.The results of HE staining in bone samples of the four groups showed that 4 rats in the experimental group and 2 rats in the control group a showed that the bone tissue on the cross section was basically intact, the bone marrow tissue was abundant or relatively abundant, and a small amount of bone tissue interlaced with the bone marrow tissue.In the blank group and the control group, the staining of b rats showed that the bone tissue was damaged to different degree and the bone marrow tissue was rare or deficient. 3.X ray films of the limbs of the four groups showed that 4 of the 5 rats in the experimental group were basically healed, 2 of the 5 rats in the control group were basically healed, and the X ray of the healed tibia showed the bone cortex of the original defect of the tibia was continuous.No fracture line was found in the control group b and the right tibia was not completely healed in the control group (b) and the right tibia in the blank group was not completely healed. The bone cortex of the middle and lower tibia was not connected, the fracture line was obvious, and there was no obvious callus growth around it.Conclusion: both external application and pulsed electromagnetic field therapy can reexpress endogenous BMP-2 in the descending trend, induce bone repair and promote the healing of bone defect.But the external application of Xiegudan was superior to pulse electromagnetic field therapy in regulating BMP-2.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

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