依达拉奉和右美托咪定对体外循环大鼠的脑保护作用及机制研究

发布时间：2018-04-21 12:04

本文选题：依达拉奉 + 体外循环　；参考：《江苏大学》2017年硕士论文

【摘要】：第一部分:依达拉奉对体外循环大鼠脑损伤的保护作用目的依达拉奉是新型氧自由基清除剂,本研究拟观察依达拉奉对体外循环(CPB)所致大鼠脑损伤的影响。方法雄性成年SD大鼠24只,体重400～450 g,随机均分为三组(n=8/组),分别为假手术组(S组)、CPB组(C组)和依达拉奉组(E组)。S组穿刺但不进行CPB;C组建立CPB模型;E组建立CPB模型并在预冲液中加入依达拉奉5 mg/kg。CPB结束后24 h经股动脉抽血检测血清中白血病介素(interleukin-6,IL-6)、IL-10、神经元特异性烯醇化酶(neuron specific enolase,NSE)和S-100β钙结合蛋白的含量;取脑组织裂解匀浆,测丙二醛(Malondialdehyde,MDA)的含量和超氧化歧化酶(Superoxide dismutase,SOD)的活性。结果与S组相比,C组和E组的IL-6、IL-10、NSE、S-100β以及MDA含量明显增高(P0.05),SOD活性明显降低(P0.05)。与 C 组比较,E 组 IL-6、NSE、S-100β 和 MDA 含量明显降低(P0.05),IL-10含量和SOD活性明显升高(P0.05)。结论预冲液中加入5mg/kg依达拉奉可减轻CPB所致大鼠脑炎症和氧化应激反应,对CPB大鼠具有明显的脑保护作用。第二部分:右美托咪定对体外循环大鼠神经元凋亡的影响目的右美托咪定是高选择性α 2受体激动剂,本研究探讨右美托咪定对心肺转流(CPB)大鼠神经元凋亡的影响。方法雄性SD大鼠24只随机均分为以下三组(n=8/组):假手术组(S组)、CPB组(C组)和右美托咪定组(D组)。S组穿刺但不进行CPB;C组建立CPB模型;D组CPB前20 min静脉注射右美托咪定10μg/kg。CPB结束后24 h时处死大鼠,取脑组织,采用TUNEL法检测凋亡细胞,计算凋亡指数,采用免疫组化法检测Bcl-2和Bax的表达,计算Bcl-2/Bax比值。结果与S组相比,C组和D组凋亡指数明显升高,Bcl-2和Bax表达上调及Bcl-2/Bax比值升高(P0.05);与C组相比,D组凋亡指数降低,Bcl-2表达上调,Bax表达下调,Bcl-2/Bax升高(P0.05)。结论预先静脉注射右美托咪定10 μg/kg可抑制CPB大鼠神经元凋亡,其机制可能与调节Bcl-2和Bax表达有关,具有明显的神经保护作用。
[Abstract]:Part I: protective effects of Edaravone on brain injury in rats with cardiopulmonary bypass objective to investigate the effects of Edaravone on brain injury induced by CPB in rats. Methods Twenty-four adult male SD rats were used. Weight 400g, each group was randomly divided into three groups: sham operation group (group S) and Edaravone group (group E). Group S was punctured but no CPB model was established in group C (group E). CPB model was established in group E and Edaravone was added to the pre-flushing fluid. 5After the end of mg/kg.CPB, the contents of IL-10, neuron specific enolase neuron specific enolase (NSE) and S-100 尾 calcium-binding protein (S-100 尾 -calcium-binding protein) in serum were detected by femoral artery blood sampling. The content of malondialdehyde (malondialdehyde) and the activity of superoxide dismutase (SOD) were measured. Results compared with S group, the contents of IL-6, IL-10, NSES-100 尾 and MDA in group C and E were significantly higher than those in group S, and the activity of sod in group C and group E was significantly lower than that in group E. Compared with group C, the contents of IL-6NSES-100 尾 and MDA in E group significantly decreased the content of IL-10 and the activity of SOD increased significantly. Conclusion the addition of 5mg/kg Edaravone to the preflushing solution can reduce the inflammation and oxidative stress in the brain induced by CPB in rats, and has a significant protective effect on the brain of CPB rats. Part 2: effects of dexmetomidine on neuronal apoptosis in cardiopulmonary bypass rats objective to investigate the effect of dexmetomidine on apoptosis of cardiopulmonary bypass (CPB) neurons in rats with cardiopulmonary bypass (CPB). Methods Twenty-four male Sprague-Dawley rats were randomly divided into the following three groups: sham operation group (group S) and right metoimidine group (group D). The rats were killed 24 hours after the end of 10 渭 g/kg.CPB. The apoptotic cells were detected by TUNEL method and the apoptotic index was calculated. The expression of Bcl-2 and Bax was detected by immunohistochemical method and the ratio of Bcl-2/Bax was calculated. Results compared with group S, the apoptotic index of C group and D group were significantly increased, the expression of Bcl-2 and Bax and the ratio of Bcl-2/Bax were increased (P 0.05), and the expression of Bcl-2 / Bax was down-regulated in group C (P 0.05), and the expression of Bcl-2 / Bax was down-regulated in group C (P < 0.05). Conclusion intravenous dexmetidine 10 渭 g/kg can inhibit neuronal apoptosis in CPB rats. The mechanism may be related to regulating the expression of Bcl-2 and Bax, and has obvious neuroprotective effect.
【学位授予单位】：江苏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R654.1

【参考文献】