趋化因子CXCL13对骨性关节炎HS细胞中RANKL表达的影响

发布时间：2018-04-27 08:50

本文选题：骨性关节炎 + CXCL13　；参考：《延边大学》2017年硕士论文

【摘要】：目的:观察CXCL13对RANKL在骨性关节炎滑膜细胞中的表达的影响,探讨两者在骨性关节炎滑膜细胞中的相关性。方法:第一部分实验利用TNF-α处理正常关节滑膜细胞,应用免疫荧光检测滑膜细胞中CXCL13的表达;第二部分实验使用CXCL13处理骨性关节炎滑膜细胞,运用蛋白免疫印迹分析方法检测经过CXCL13(不同浓度、不同时间)处理后的骨性关节炎滑膜细胞中RANKL蛋白的表达。结果:1.免疫荧光结果显示CXCL13在骨性关节炎滑膜细胞中表达明显低于正常人的滑膜细胞。2.利用TNF-α(浓度10ug/L)处理正常关节滑膜细胞,随着作用时间的延长CXC113表达呈现减弱趋势。3.蛋白免疫印记检测结果显示:在骨性关节炎滑膜细胞中RANKL蛋白的表达量明显高于正常人滑膜细胞;经TNF-α(浓度为10ng/ml)处理24h后,更换含有CXCL13(浓度25ng/ml)的培养液继续培养1h、3h、6h、24h后,发现在骨性关节炎滑膜细胞中RANKL蛋白表达水平随着时间的延长而降低。经TNF-α(浓度10ng/ml)处理24h后,在更换含有不同浓度的趋化因子CXCL13(Ong/ml、5ng/ml、15ng/ml、25ng/ml)培养液继续处理24h后,RANKL蛋白的表达量随着浓度的增加而降低。在骨性关节炎滑膜细胞中RANKL的蛋白表达量随着CXC113刺激浓度的增加及作用时间的延长,呈现时间依赖性及浓度依赖性降低。结论:CXCL13在一定浓度和时间能够对RANKL表达进行抑制,从而可能通过这一途径进一步延缓骨性关节炎症的炎症进展、骨质破坏、软骨硬化等症状。
[Abstract]:Aim: to observe the effect of CXCL13 on the expression of RANKL in synovial cells of osteoarthritis and to explore the correlation between RANKL and RANKL in synovial cells of osteoarthritis. Methods: normal synovial cells were treated with TNF- 伪 and the expression of CXCL13 in synovial cells was detected by immunofluorescence. In the second part, CXCL13 was used to treat synovial cells of osteoarthritis. The expression of RANKL protein in synovial cells of osteoarthritis treated with CXCL13 (different concentrations and different time) was detected by Western blot. The result is 1: 1. Immunofluorescence results showed that the expression of CXCL13 in synovial cells of osteoarthritis was significantly lower than that in normal synovial cells. Normal synovial cells were treated with TNF- 伪 (10 ug- / L), and the expression of CXC113 decreased with the prolongation of time. The expression of RANKL protein in synovial cells of osteoarthritis was significantly higher than that in normal synovial cells, and after 24 hours of TNF- 伪 (10 ng / ml) treatment, the culture medium containing CXCL13 (25 ng / ml) was continuously cultured for 1 h and 3 h for 6 h. It was found that the expression of RANKL protein decreased with time in synovial cells of osteoarthritis. After being treated with TNF- 伪 (10 ng / ml) for 24 h, the expression of RANKL protein decreased with the increase of concentration of CXCL13 Ong / ml 5 ng / ml ~ (15) ng / ml ~ (25) ng 路ml ~ (-1) after 24 h treatment with TNF- 伪 (concentration 10 ng / ml). The expression of RANKL protein in synovial cells of osteoarthritis decreased in a time-and concentration-dependent manner with the increase of CXC113 stimulation concentration and the prolongation of the time of action. Conclusion the expression of RANKL can be inhibited at a certain concentration and time, which may further delay the progression of inflammation, bone destruction and cartilage sclerosis in osteoarthritis.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R684.3

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