FODMAPs在健康人群体内外发酵的对比研究

发布时间：2018-04-30 01:10

本文选题：FODMAPs + 氢呼气试验　；参考：《浙江大学》2017年硕士论文

【摘要】：背景与目的:可酵解的低聚糖、双糖、单糖和多元醇(FODMAPs)是食品中常见一类的碳水化合物,因其可被结肠细菌发酵产生气体和短链脂肪酸(SCFA),从而可能诱发包括肠易激综合征等功能性胃肠病患者产生消化道症状。为了研究FODMAPs在人肠道内的发酵情况,可采用体内或体外两种研究方式。体内发酵研究可以真实反应肠道菌群的发酵情况,但却费时繁琐;而在体外发酵研究相对省时便捷,但却不可能完全模拟出肠道内环境情况。目前关于不同FODMAPs的发酵情况以及体外批量发酵系统准确性评估的研究尚为有限。方法:2016年9月至2017年2月通过网络招募健康志愿者,受试者完成3次氢呼气试验,底物分别为低聚果糖(FOS)、低聚半乳糖(GOS)和木糖醇(XYI),检测4小时内呼气中的氢浓度;并在每次试验的第0、2小时分别留取静脉血样本,测定SCFA含量。同时收集受试者粪便样本进行体外发酵,分别选用FOS培养基、GOS培养基和XYI培养基,并用基础培养基(YCFA)作为对照;发酵48小时,取第0、24和48小时样本测定SCFA含量;并测定第24小时培养瓶内压强。分析不同底物发酵后的气体和SCFA变化情况,同时比较体内外发酵在气体增量和SCFA增量上的相关性。结果:共纳入15例健康志愿者,男性7例,女性8例,平均年龄37.53±13.11岁。(1)FOS 4 小时氢气呼出总量为 3845±3207.10ppm,GOS 为 2734.13±2215.78ppm,两者均高于XYI的564.0±458.76ppm,差异具有统计学意义(p0.05);但血浆SCFA在摄入底物2小时后的浓度较基础值变化不明显。FOS、GOS、XYI体外发酵第24小时的压强无显著差异,但XYI发酵48小时的产丙酸、丁酸量均显著高于FOS和GOS(p0.05)。(2)FODMAPs在不同个体间发酵水平差异较大。FOS体外发酵压强的最大值为56.9KPa,最小值为4.0kPa,平均数为18.47KPa,GOS分别为 53.2、-0.7、15.73KPa;XYI 分别为 30.5、3.0、11.76KPa。FOS 体外发酵48小时产总酸量的最大值为54.98mmol/L,最小值为4.51mmol/L,平均数为26.21mmol/L;GOS 分别为 53.36、7.99、30.55mmol/L;XYI 分别为 80.91、35.86、51.74mmol/L。(3)氢呼气试验4小时产氢气总量和体外发酵24小时压强呈正相关(r=0.438,p0.01);但体内发酵2小时和体外发酵24小时或48小时的SCFA生成量均未发现一致性。结论:FODMAPs在人体内的发酵能力因着其种类和不同个体而有所差异;FODMAPs在体外批量的发酵研究结果可以在一定程度上反映对应底物在人体内的发酵情况。
[Abstract]:Background & objective: glycolytic oligosaccharides, disaccharides, monosaccharides and polyol FODMAPsare common carbohydrates in foods. It may induce gastrointestinal symptoms in patients with functional gastrointestinal diseases, including irritable bowel syndrome, because it can produce gas and short chain fatty acid (SCFAA) by colonic bacteria fermentation. In order to study the fermentation of FODMAPs in human intestinal tract, in vivo or in vitro, two kinds of research methods can be used. Fermentation in vivo can truly reflect the fermentation of intestinal flora, but it is time-consuming and cumbersome. In vitro fermentation is relatively time-saving and convenient, but it is impossible to completely simulate the intestinal environment. At present, studies on fermentation conditions of different FODMAPs and accuracy evaluation of batch fermentation system in vitro are limited. Methods: healthy volunteers were recruited through internet from September 2016 to February 2017. The subjects completed three hydrogen breath tests, the substrates were fructose oligosaccharide (FOS), galactooligosactooligosactooligosaccharide (GOS) and xylitol (XYI), and the hydrogen concentration in the breath was measured within 4 hours. Venous blood samples were collected at 0 h of each trial to determine the content of SCFA. At the same time, the fecal samples were collected for in vitro fermentation. The FOS medium and XYI medium were used as control medium, respectively, and the SCFA content was measured at the 24th and 48th hour after 48 hours of fermentation. The pressure in culture bottle at 24 hours was measured. The changes of gas and SCFA after fermentation with different substrates were analyzed, and the correlation between gas increment and SCFA increment in vitro and in vivo was compared. Results: a total of 15 healthy volunteers were included, including 7 males and 8 females. The average age was 37.53 卤13.11 years old. The total exhalation of hydrogen gas was 3845 卤3207.10 ppm-1 路h, 2734.13 卤2215.78 ppm, both were higher than 564.0 卤458.76 ppm of XYI, the difference was statistically significant (p 0.05), but the concentration of plasma SCFA was not significantly different from the basic value after 2 hours of substrate intake. There was no significant difference in pressure at the 24th hour, But XYI fermented for 48 hours to produce propionic acid, The content of butyric acid was significantly higher than that of FOS and GOS(p0.05).(2)FODMAPs. The maximum pressure of in vitro fermentation was 56.9 KPA, the minimum value was 4.0 KPA, and the average value was 18.47 KPa-15.73KPa-XYI was 30.53.011.76KPa.FOS produced total acid in vitro for 48 hours. The maximum value is 54.98 mmol / L, the minimum value is 4.51 mmol / L, the average is 26.21 mmol / L, the average GOS is 53.36 mg / L 7.99 渭 g / L 30.55 mmol / L, respectively. The XYI of hydrogen exhalation test is 80.91g / L 35.86 mg / L 51.74 mmol / L 路L ~ (3) hydrogen production in vitro has a positive correlation with the pressure of 24 h fermentation in vitro, but the in vivo fermentation for 2 hours and in vitro fermentation for 24 hours or No consistency was found in 48 hours of SCFA production. Conclusion the fermentation ability of FODMAPs in human body is different according to its species and individuals. The results of batch fermentation of FODMAPs in vitro can reflect the fermentation of corresponding substrates in human body to some extent.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R57

【相似文献】