基于二氧化硅纳米颗粒构建智能载药体系及其生物应用研究

发布时间：2018-05-01 06:24

本文选题：二氧化硅 + 载体分解　；参考：《南京理工大学》2017年硕士论文

【摘要】：理想的载药材料具有在生物体内可降解和可控释放性能,本文以二氧化硅纳米材料为药物载体,制备了载体可分解材料和多条件药物控释体系。研究分为两个部分:(1)介绍了一种酸/碱双刺激响应二氧化硅/抗癌药物杂化载药体系(MSNPs-1),由抗癌药物阿霉素(DOX)和二氧化硅通过改进的Stober法制得。用扫描透射电子显微镜等测定MSNPs-1结构。用透射电镜和紫外-可见分光光度计对其在不同pH条件下药物释放情况进行研究,证明其在酸性或碱性溶液中可响应释放药物,同时载体分解。细胞实验证明MSNPs-1在7721细胞中可缓控释放药物,为其在治疗生物体内肿瘤方面的应用提供理论基础。(2)介绍了一种具有pH和紫外光双响应性质的智能纳米容器(MSNPs-2),其制备方法是在MCM-41表面修饰含有腙键结构和偶氮苯衍生物识别位点的功能性分子链以及大环分子α-环糊精。具体工作如下:合成4-肼基甲基偶氮苯,在光控条件下研究其与0α-环糊精的自组装行为。在制备的MCM-41表面修饰醛基三乙氧基硅烷和4-肼基甲基偶氮苯,反应形成腙键,用α-环糊精将DOX药物分子封装在介孔中。采用透射电镜等对改性的纳米材料进行表征。使用紫外-可见分光光度计研究MSNPs-2在酸性条件和紫外光照射下药物释放情况,验证了 MSNPs-2的pH和光双响应性能。MCF-7细胞实验证明载体细胞毒性较小,在紫外光照射条件下可在细胞内快速释放药物,实现药物在癌细胞环境中的有效释放,在生物医药领域有潜在的应用价值。
[Abstract]:The ideal drug carrier has biodegradable and controllable release properties in vivo. In this paper, the carrier decomposable material and multi-conditional drug controlled release system were prepared with silica nanomaterials as drug carrier. The study was divided into two parts: (1) an acid / alkali responsive silica / anticancer drug hybrid drug delivery system was introduced, which was obtained by the modified Stober method with the anticancer drug adriamycin (DOX) and silicon dioxide (SiO2). The structure of MSNPs-1 was determined by scanning transmission electron microscope (SEM). Transmission electron microscopy and UV-Vis spectrophotometer were used to study the drug release under different pH conditions. It was proved that the drug could be released in acidic or alkaline solution and the carrier could be decomposed at the same time. Cell experiments showed that MSNPs-1 could release drugs slowly in 7721 cells. To provide a theoretical basis for its application in the treatment of tumor in vivo, this paper introduces an intelligent nanovessel with both pH and UV response properties, which is prepared by modifying the surface of MCM-41 with Hydrazone structure and azobenzene. Functional molecular chains and macromolecular 伪-cyclodextrins of derivative recognition sites. The main work is as follows: 4-hydrazo-methylazobenzene was synthesized and its self-assembly behavior with 0 伪 -cyclodextrin was studied under light control. The surface of MCM-41 was modified with aldehyde triethoxy silane and 4-hydrazo-methylazobenzene to form Hydrazone bond. DOX was encapsulated in mesoporous cells by 伪 -cyclodextrin. The modified nanomaterials were characterized by transmission electron microscopy (TEM). UV-Vis spectrophotometer was used to study the drug release of MSNPs-2 under acidic and ultraviolet irradiation. The pH and photoresponse of MSNPs-2. MCF-7 cell experiment showed that the cytotoxicity of MSNPs-2 was less than that of MCF-7 cells. The drug can be released rapidly in cells under ultraviolet irradiation, which can effectively release the drug in the environment of cancer cells. It has potential application value in the field of biomedicine.
【学位授予单位】：南京理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R943

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