Toll样受体mRNA和相关因子在肝泡型包虫病人体内的变化

发布时间：2018-05-15 13:14

  本文选题：肝泡型包虫病 + Toll样受体　； 参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:探讨Toll样受体2(Toll-like receptor 2,TLR2)和TLR4 m RNA在肝多房棘球蚴病(hepatic alveolar echinococcosis,HAE)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)和肝组织中的表达情况及其与血浆相关细胞因子水平之间的相关性。方法:收集肝多房棘球蚴病患者(HAE组)28例和健康志愿者作为对照(HC组,n=28例),用ELISA检测血浆中γ干扰素(IFN-γ)、白细胞介素5(IL-5)、IL-23、IL-10水平,用q RT-PCR法检测PBMCs以及HAE患者肝组织中TLR2和TLR4m RNA的表达水平,外周血嗜酸粒细胞百分比(Eo%)用血细胞分析仪检测。Spearman秩相关分PBMCs中TLR2和TLR4 m RNA水平与血浆中细胞因子水平和外周血Eo%的相关性。结果:ELISA检测结果显示,HAE组血浆中IFN-γ、IL-5、IL-23、IL-10水平分别为(301.100±47.290)、(43.420±11.380)、(86.580±31.990)、(8.766±7.568)pg/ml,均高于HC组的(301.100±67.790)、(40.970±6.310)、(46.770±15.490)、(6.272±10.360)pg/ml,其中两组的IL-23水平之间的差异性有统计学意义(P0.01)。q RT-PCR检测结果显示,HAE组PBMCs中TLR2 m RNA相对表达量为0.100±0.084,高于HC组的0.055±0.040(P0.05);HAE组和HC组的TLR4 m RNA相对表达量分别为0.004±0.003和0.003±0.002,两者间差异无统计学意义(P0.05)。HAE患者肝病灶组织中TLR2和TLR4 m RNA相对表达量分别为29.680±25.650和21.340±16.640,均高于病灶旁的2.308±4.140和5.541±9.233以及正常组织的1.112±1.431和1.100±1.734(P0.01)。HAE组和HC组外周血Eo%分别为0.448±0.240和0.110±0.100,两者差异有统计学意义P0.01)。Spearman秩相关分析结果显示,PBMCs中TLR2 m RNA水平与血浆中IL-23水平和外周血Eo%之间均存在正相关关系(r分别为0.368和0.382)。结论:肝多房棘球蚴病患者PBMCs、肝病灶中的TLR2和TLR 4m RNA表达水平升高,PBMCs中TLR2 m RNA水平与血浆中IL-23水平和外周血Eo%之间均存在正相关关系。这与棘球蚴绦虫在感染人体干预宿主的免疫调节系统并产生免疫耐受、免疫逃避和致病有关。
[Abstract]:Objective: to investigate the expression of Toll like receptor 2(Toll-like receptor 2 (TLR2) and TLR4 m RNA in peripheral blood mononuclear cells (PBMC) and liver tissues of patients with hepatic alveolar echinococcus coensis (HAE) and their correlation with plasma cytokines levels. Methods: the plasma levels of IFN- 纬 and IL-23 IL-10 were measured by ELISA in 28 patients with hepatic hydatidosis and 28 healthy volunteers as control group. The expression of TLR2 and TLR4m RNA in the liver tissues of PBMCs and HAE patients was detected by Q RT-PCR method. The correlation between the levels of TLR2 and TLR4 m RNA in PBMCs and the levels of cytokines in plasma and Eo% in peripheral blood were detected by blood cell analyzer. 缁撴灉:ELISA妫，

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