霍山石斛多糖对香烟烟雾暴露致小鼠肺部炎症的预防研究

发布时间：2018-05-15 16:42

本文选题：霍山石斛 + 多糖　；参考：《合肥工业大学》2017年硕士论文

【摘要】：霍山石斛是我国传统的可食用中草药,具有增强机体免疫、抗氧化、抗肿瘤、改善肺功能等多种生物活性。本文主要开展了霍山石斛多糖(DHP)对香烟烟雾暴露致小鼠肺部炎症的预防机制研究。取得的主要研究结果如下:(1)DHP对香烟烟雾暴露致小鼠的表观现象和指标的影响:小鼠体重变化及脏器指数结果表明,香烟烟雾暴露可明显减轻小鼠体重,降低食物摄入量,造成肺组织、肝脏、脾脏和肾脏的损伤;DHP可有效恢复小鼠的体重和食物摄入量,抑制脏器的损伤。(2)DHP对香烟烟雾暴露致小鼠肺部炎症的预防分子机制:小鼠肺组织HE染色研究显示,DHP能显著抑制香烟烟雾诱导的小鼠肺组织炎症细胞浸润,明显降低平均内衬间隔和肺泡破坏指数,从而减轻小鼠肺泡破坏情况;巨噬细胞CD68免疫组化表明DHP能明显减少巨噬细胞在肺组织气管内的聚集。ELISA实验表明,DHP能显著抑制香烟烟雾暴露致小鼠血清和肺组织中促炎因子TNF-α和IL-1β的产生。当DHP剂量为400 mg/kg·day时,血清TNF-α和IL-1β的水平及肺组织中TNF-α和IL-1β的含量分别比模型组减小了13.6%、35.3%、20.2%和21.8%。Western Blot分析表明,DHP能显著抑制香烟烟雾暴露致小鼠肺损伤中炎性通路MAPK和NF-κB信号通路特征性蛋白P65、P38和JNK的磷酸化程度及IκB的降解,且呈剂量效应关系。EMSA结果表明,DHP可有效阻断香烟烟雾暴露致小鼠肺损伤中NF-κB和AP-1的DNA结合活性,且结合活性随着浓度的增大而逐渐降低。综上所述,DHP通过改善表观现象,调控炎性信号通路,从而抑制炎性因子的过度分泌,抑制肺部炎症的发生。
[Abstract]:Dendrobium huoshanense is a traditional edible Chinese herbal medicine with many biological activities, such as enhancing immunity, anti-oxidation, anti-tumor, improving lung function and so on. The preventive mechanism of DHP on lung inflammation induced by cigarette smoke exposure in mice was studied. The main results were as follows: the effects of DHP on the apparent phenomena and indexes of mice exposed to cigarette smoke: the results of body weight change and viscera index showed that cigarette smoke exposure could significantly reduce the weight and food intake of mice. DHP can effectively recover the body weight and food intake of mice caused by damage to lung tissue, liver, spleen and kidney. The molecular mechanism of inhibiting the lung inflammation induced by cigarette smoke exposure: the study of HE staining in mouse lung tissue showed that DHP could significantly inhibit the infiltration of inflammatory cells in lung tissue induced by cigarette smoke. The average lining interval and alveolar destruction index were significantly decreased, so as to alleviate the alveolar destruction in mice. CD68 of macrophages showed that DHP could significantly reduce the aggregation of macrophages in the trachea of lung tissue. Elisa showed that DHP could significantly inhibit the production of TNF- 伪 and IL-1 尾 in serum and lung tissues of mice exposed to cigarette smoke. When the dose of DHP is 400 mg/kg day, The levels of TNF- 伪 and IL-1 尾 in serum and the contents of TNF- 伪 and IL-1 尾 in lung tissue in the model group were significantly decreased by 13.6% and 35.30.2% than those in the model group, respectively. 21.8%.Western Blot analysis showed that DHP could significantly inhibit the inflammatory pathway MAPK and NF- 魏 B signaling pathway characteristic eggs in lung injury induced by cigarette smoke exposure in mice. The phosphorylation degree of P65, P38 and JNK and the degradation of I 魏 B, The results showed that DHP could effectively block the DNA binding activity of NF- 魏 B and AP-1 in mice lung injury induced by cigarette smoke exposure, and the binding activity decreased with the increase of concentration. To sum up, DHP inhibits the excessive secretion of inflammatory factors and the occurrence of pulmonary inflammation by improving the apparent phenomenon and regulating the inflammatory signaling pathway.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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