化湿降浊方干预高尿酸血症模型大鼠作用的实验研究

发布时间：2018-05-22 18:56

本文选题：高尿酸血症模型 + 化湿降浊　；参考：《南京中医药大学》2017年硕士论文

【摘要】：目的:探研导师经验方"化湿降浊方"对高尿酸血症模型大鼠的干预作用及部分作用机制,为中药复方的临床应用提供一定客观依据。方法:采用灌胃次黄嘌呤联合氧嗪酸钾腹腔注射[1]连续7d以复制高尿酸血症模型大鼠。同时予以别嘌呤醇为阳性药物对照,研究化湿降浊方(1.2g.ml-1)对各组血尿酸(UA)、肝脏黄嘌呤氧化酶(xanthine oxidase,XOD)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的影响;采用同样方法7d复制高尿酸血症模型大鼠,再予苯溴马隆为阳性药物对照,化湿降浊方(1.2g.ml-1)对各组灌胃7d,检测其对血尿酸(UA)、尿UA、尿素氮(BUN)、肌酐(Scr)的影响,并观察肾组织切片变化。运用SPSS17.0统计软件对上述数据进行分析,以P0.05为差异有统计学意义。结果:1.与空白组对比,模型组大鼠血UA显著升高(P0.01)。与模型组相比,中药组能有效抑制模型大鼠肝脏XOD的活性以达到降低血UA的作用,其差异具有显著性(P0.01);中药组较别嘌呤醇组显著降低ALT、AST含量(P0.05,P0.01)。2.与空白组相比,苯溴马隆组、中药组均能有效促进模型大鼠尿UA排泄,达到降低血UA的作用,其差异具有显著性(P0.01);中药组较模型组的血清Scr、BUN含量降低(P均0.01);3.肾组织切片方面:化湿降浊组及苯溴马隆组对模型大鼠肾脏的损害程度均较模型组轻,其中化湿降浊组对模型大鼠肾间质损害度较苯溴马隆组轻,仅见轻度水肿伴少量尿酸结晶形成。结论:1.化湿降浊方能有效降低高尿酸血症模型大鼠的血尿酸水平。2.化湿降浊方能够有效抑制高尿酸血症模型大鼠肝脏黄嘌呤氧化酶的活性,并且不会引起ALT、AST的异常。3.化湿降浊方具有促进高尿酸血症模型大鼠尿酸排泄作用,有一定程度的肾功能保护作用。
[Abstract]:Objective: to explore the intervention effect and partial mechanism of "Huashi Jiangzhuo prescription" on hyperuricemia model rats, and to provide some objective basis for clinical application of traditional Chinese medicine prescription. Methods: the hyperuricemia model rats were induced by intraperitoneal injection of Hypoxanthine and potassium oxazinate for 7 days. At the same time, allopurinol was given as positive drug control, and the effects of Huazhi Jiangzhuo prescription (1.2g 路ml-1) on serum uric acid, xanthine oxidase XODN, alanine aminotransferase (alt) and aspartate aminotransferase (AST) were studied in rats with hyperuricemia by the same method for 7 days. In addition, benzbromarone was used as the positive drug control, and Huashi Jiangzhuo prescription (1.2g 路ml-1) was given to each group for 7 days, and the effects on serum uric acid, urine UAA, urea nitrogen bun, creatinine Scr1) were detected, and the changes of renal tissue sections were observed. Using SPSS17.0 statistical software to analyze the above data, P0.05 as the difference is statistically significant. The result is 1: 1. Compared with the blank group, the serum UA in the model group was significantly higher than that in the control group (P 0.01). Compared with the model group, the Chinese medicine group could effectively inhibit the activity of XOD in the liver of the model rats to achieve the effect of reducing the blood UA, the difference was significant (P 0.01), and the content of alt in the Chinese medicine group was significantly lower than that in the allopurinol group. Compared with the blank group, the traditional Chinese medicine group could effectively promote the urinary UA excretion of the model rats, and achieve the effect of reducing the blood UA level, the difference was significant (P 0.01), and the serum Scr-bun content in the Chinese medicine group was lower than that in the model group (P < 0.01). In the aspect of renal tissue section, the damage degree of kidney in dehumidification and turbid group and benzbrommalone group was lighter than that in model group, and the degree of renal interstitial damage in dehumidification and turbid group was lighter than that in benzbromine group. Mild edema was observed with a small amount of uric acid crystallization. Conclusion 1. Huashi Jiangzhuo prescription can effectively reduce the blood uric acid level of hyperuricemia rats. 2. 2. Huashi Jiangzhuo prescription can effectively inhibit the activity of xanthine oxidase in liver of hyperuricemia rats and does not cause the abnormality of alt AST. 3. Huashi Jiangzhuo prescription can promote the excretion of uric acid in hyperuricemia rats and protect renal function to some extent.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5;R-332

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