血液病患者出血程度与血小板微颗粒、血管性血友病因子、纤维连接蛋白的关系及临床意义

发布时间：2018-06-03 11:28

本文选题：出血程度 + 血小板微颗粒　；参考：《安徽医科大学》2017年硕士论文

【摘要】：目的观察血小板微颗粒(Platelet microparticle,PMP)、血管性血友病因子(von Willebrand Factor,v WF)、纤维连接蛋白(Fibronecti,FN)在血小板减少的血液病患者中与出血程度的关系;探讨PMP、v WF、FN对出血程度预测的临床意义.方法病例均采集于2016年1月~2016年6月安徽医科大学第一附属医院血液内科血小板计数20×109/L的患者90人作为试验组,所有疾病的诊断均符合张之南主编的《血液病诊断及疗效标准》中的诊断标准,参照WHO关于出血的分级标准,根据试验组患者出血程度分为0分组、1分组、2分组、3分组、4分组。以同时期我院体检中心体检人50例为对照组,试验组与对照组在年龄,性别方面均无统计学差异。所有试验组及对照组人群均无心脑血管疾病,试验前2周内未服用抗凝及抗血小板药物,未进行输血治疗。应用流式细胞术(FCM)检测患者外周血中血小板微颗粒含量变化,应用酶联免疫吸附测定法(ELISA)检测患者外周血中血管性血友病因子、纤维连接蛋白含量变化。结果(1)各组血小板微颗粒、血管性血友病因子、纤维连接蛋白的含量均有显著差异(P0.001)(2)不同出血程度组之间出血程度越重血小板微颗粒含量越低(1分组与2分组;3分组、4分组和对照组无明显统计学差异,其余有统计学差异)、血管性血友病因子含量越高(除1分组和2分组外,其余各组之间差异均有显著统计学意义)纤维连接蛋白含量越高(除0分组与1分组外,其余各组均有显著统计学差异)。结论血小板微颗粒、血管性血友病因子、纤维连接蛋白的含量对血小板减少的血液病患者出血程度的预测具有临床意义。
[Abstract]:Objective to investigate the relationship between platelet microparticle PMPN, von Willebrand Factorv WFN, fibronectidine FN in patients with thrombocytopenia, and the clinical significance of PMPV WFFN in predicting the degree of hemorrhage in patients with thrombocytopenia. Methods from January 2016 to June 2016, 90 patients with 20 脳 109 / L platelet count in Department of Hematology, first affiliated Hospital of Anhui Medical University, were selected as trial group. The diagnosis of all diseases was in accordance with the criteria of diagnosis and curative effect of Hematological Diseases edited by Zhang Zhinan. According to the WHO classification standard of bleeding, the patients in the experimental group were divided into 0 groups, 1 group, 2 groups, 2 groups, 3 groups, 4 groups. There were no significant differences in age and sex between the test group and the control group, taking 50 cases of physical examination in the same period as the control group. No cardiovascular and cerebrovascular diseases were found in all the experimental and control groups. No anticoagulant and antiplatelet drugs were taken within 2 weeks before the trial. Flow cytometry (FCM) was used to detect the changes of platelet microparticles in peripheral blood, and enzyme linked immunosorbent assay (Elisa) was used to detect the changes of von Willebrand factor and fibronectin in peripheral blood. Results (1) Platelet microgranule, von Willebrand factor, There were significant differences in the contents of fibronectin (P 0.001) the more severe the degree of hemorrhage was, the lower the platelet microparticle content was. There was no significant difference between group 1 and group 2, group 3, group 4, group 4 and control group. There were statistical differences in the other groups. The higher the content of von Willebrand factor (except group 1 and group 2, the difference between the other groups were statistically significant), the higher the content of fibronectin (except group 0 and group 1). There were significant statistical differences in other groups. Conclusion the contents of platelet microparticles, von Willebrand factor and fibronectin have clinical significance in predicting the bleeding degree of patients with thrombocytopenia.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R55

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