何首乌的化学成分研究

发布时间：2018-06-09 01:10

本文选题：二苯乙烯苷 + 二蒽酮　；参考：《北京中医药大学》2017年硕士论文

【摘要】：目的:传统植物药何首乌(Radix Polygoni Mulfiflori)为寥科(Polygonaceae),何首乌属(Fallopia Adans)多年生草本植物何首乌Polygonum multiflorum Thunb.的块根。本论文对近年来何首乌的化学成分和药理活性的研究进行了较为全面概括,发现近年来对何首乌抗糖尿病活性的有效成分并不明确,因此本课题对何首乌具有抗糖尿病活性的55%乙醇部位进行了研究,以期能发现具有活性的单体化合物。方法:运用现代各种色谱技术(大孔吸附树脂、SephadexLH-20、反相硅胶柱色谱和液相制备)对何首乌55%乙醇部位进行分离纯化,运用核磁共振等光谱技术对化合物进行结构鉴定,并对分离得到的化合物进行了降血糖活性筛选。结果:从何首乌中共分离鉴定了 27个化合物:Thunberginol C 6-O-β-D-glucopyran-oside(1),2,3,5,4'-四羟基二苯乙烯-2-O-β-L-鼠李糖苷(2),对羟基苯甲醛(3),反式-N-咖啡酰酪胺(4),白藜芦醇-3-O-(2"-O-没食子酰基)-β-D-葡萄糖苷(5),2,3,5,4'-四羟基二苯乙烯-2-0-(3〃-没食子酰基)-β-D-葡萄糖苷(6),决明酮-8-O-β-D-葡萄糖苷(7),6-hydroxymusizin-8-O-β-D-glucoside(8),2,3,5,4'-四羟基二苯乙烯-2-O-(2〃-对羟基苯甲酰基)-β-D-葡萄糖苷(9),2,3,5,4'-四羟基二苯乙烯-2-0-(2〃-没食子酰基)-β-D-葡萄糖苷(10),反式-2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(11),顺式-2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(12),苜蓿素-6-0-葡萄糖苷(13),大黄素-8-O-β-D-葡萄糖苷(14),穆坪马兜铃酰胺(15),N-反式阿魏酰基-3-甲基多巴胺(16),槲皮素(17),苜蓿素(18),何首乌丙素(19),儿茶素(20),表儿茶素(21),土大黄苷(22),cis-大黄素-大黄素二蒽酮(23),trans-大黄素-大黄素二蒽酮(24),白藜芦醇(25),白藜芦醇苷(26),决明酮-8-0-(6'-O-乙酰基)-β-D-葡萄糖苷(27)。结论:化合物为1、2为新化合物,化合物4、5、13、27为首次从何首乌中分离得到。对何首乌各粗提部位以及单体成分进行降血糖活性筛选,结果表明,55%乙醇部位以及化合物6、9、10、23和24具有较好的降血糖活性。
[Abstract]:Objective: Radix Polygoni Mulfiflori (Radix Polygoni Mulfiflorii) is a perennial herb of Polygonaceaeae (Fallopia Adansa) and Polygonum multiflorum Thunb. The root of In this paper, the chemical constituents and pharmacological activities of Polygonum multiflorum in recent years were summarized, and it was found that the effective components of the anti-diabetic activity of Polygonum multiflorum were not clear in recent years. Therefore, 55% ethanol fraction with antidiabetic activity of Polygonum multiflorum was studied in order to find active monomers. Methods: all kinds of modern chromatographic techniques (Sephadex LH-20, reverse silica gel column chromatography and liquid phase preparation) were used to separate and purify the 55% ethanol fraction of Polygonum multiflorum. The hypoglycemic activity of the isolated compounds was screened. Results: a total of 27 compounds: Thunberginol C 6-O- 尾 -D-glucopyran-osideside 1: Thunberginol C 6-O- 尾 -D-glucopyran-oside were isolated and identified from Radix Polygonum multiflorum. Twenty-seven compounds were isolated and identified, including Thunberginol C 6-O- 尾 -D-glucopyran-oside, tetrahydroxy distyrene -2-O- 尾 -L-rhamnoside, p-hydroxybenzaldehyde, trans--N-caffeoyltyramine, resveratrol, 3-O-O-2 "-O- 尾 -D- glucoside 235H4. Distyrene-2-0-trimethylidene 3 "- 尾 -D- glucoside 6", cassimone -8-O- 尾 -D- glucoside 7 "-hydroxymusizin-8-O- 尾 -D-glucoside 8-D-glucoside 8" -2-O-2 "-4-hydroxybenzoylformyl-尾 -dihydroxystyrene-2-O-2" -p-hydroxybenzoylformyl-尾 -D- 尾 -glucoside 933- (4-) -tetrahydroxy distyrene-2-0-2 "- 尾 -Dglucoside-尾 -glucose Glucoside 10C, trans--2O3O5- tetrahydroxy stilbene -2-O- 尾 -Dglucoside, cis-2O3O3O3O- 尾 -tetrahydroxy stilbene -2-O- 尾 -Dglucoside 12C, alfalfa -6-0- glucoside 1313, emodin -8-O- 尾 -D- glucoside 14, Aristolocham 15N- reverse ferulyl -3methylpolypolymethylethylmandeloside (Aristolochia muppingensis 15N), Alfalfa (alfalfa) -6-0- glucoside 1313, emodin-8-O- 尾-glucoside 14 Barramine 16, quercetin 17, alfalfa 18, Polygonum multiflorum 19, catechin 20, epigallocatechin 21, chrysophanoside 2 22cis-emodin dianthronone 23, emodin dianthrone 24, resveratrol 25, resveratrol 26, cassiazone -8-0-6 -O- acetyl-尾 -D- glucoside 27. Conclusion: compound 1: 1 is a new compound, and compound 4: 5, 1, 13, 27 is isolated from Radix Polygoni multiflorum for the first time. The hypoglycemic activity of crude extracts and monomers of Radix Polygoni multiflorum was screened. The results showed that 55% ethanol fraction and the compounds 60.99 10, 23 and 24 had better hypoglycemic activity.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284.1

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