“药食同源”植物提取物抗氧化功效评价

发布时间：2018-07-22 15:47

【摘要】：本文选取刺梨、干姜、肉桂、山药、葡萄籽五种天然食材为原材料,通过水提法提取有效成分,以抗氧化典型模式生物秀丽隐杆线虫(Caenorhabditis elegans,C.elegans)为模型,通过线虫寿命实验及体外氧化压力实验评估五种药食同源植物的抗氧化作用功效,从而得出所研究植物具有不同程度的抗氧化作用功效,可以通过对人体进行简单的饮食指导,达到“食补”抗衰老、抗氧化的药用价值。在“药食同源”植物研究的基础上,本文深入研究了天然植物松树的种子松花粉的抗氧化药用价值。以秀丽隐杆线虫为模型动物测定松花粉给药前后的寿命变化、热激耐受和抗氧化损伤能力。本文通过松花粉水提物对线虫的生理指标及抗氧化压力诱导的指标评估,表明2mg/ml松花粉水提物对野生型秀丽隐杆线虫N2存在显著差异的寿命影响。松花粉在喂食初期高浓度组2mg/ml产卵量高于低浓度组和空白对照组;随喂食时间的延长,低浓度喂食组1mg/ml产卵量呈现上升趋势。松花粉水提物喂食组线虫吞咽率普遍高于空白对照组,说明松花粉对于线虫生理指标的影响,不是由于热量限制导致的。高浓度松花粉2mg/ml喂食线虫对线虫运动能力影响较小,对线虫的运动能力影响优于空白对照组。此外,在氧化压力抵抗实验中,1mg/ml松花粉水提物喂食组线虫较空白对照组,有显著的热抵抗保护能力,低浓度松花粉喂食组存活率高于高浓度喂食组。表明松花粉水提物对线虫具有不同程度的抗热激氧化损伤保护作用。10mM过氧化氢诱导的体外氧化压力,松花粉水提物对线虫有良好的保护作用,且在低浓度范围内,这种保护作用与空白对照组相比即存在明显差异。表明松花粉水提物对线虫具良好的抗过氧化氢诱导氧化压力损伤保护作用。综上所述,本论文以线虫为模型,通过对“药食同源”植物的抗氧化作用评估及松花粉的抗氧化功效评价,为“药食同源”植物的抗氧化、抗衰老功效提供了实验基础。
[Abstract]:In this paper, five natural ingredients of Rosa roxburghii, dried ginger, cinnamon, yam and grape seed were selected as raw materials. The active ingredients were extracted by water extraction, and the model was Caenorhabditis elegans, a typical antioxidation model organism, Caenorhabditis eleans C. elegans. Through nematode life test and in vitro oxidation pressure test, the antioxidant effects of five drugs and food homologous plants were evaluated, and the results showed that the plants studied had different degree of antioxidant effects, and could be used to direct the diet of human body. To achieve "food supplement" anti-aging, anti-oxidation medicinal value. On the basis of the study of "Medicinal and Edible homology", the antioxidant medicinal value of pine pollen seed of natural plant pine was studied in this paper. The changes of life span, heat shock tolerance and antioxidant damage of pine pollen before and after administration were measured with the model of Stereus elegans. In this paper, the physiological indexes of water extract of pine pollen on nematode and the index of antioxidant pressure induction were evaluated. The results showed that the water extract of 2mg/ml pollen had significant difference in life span on N _ 2 of wild type cryptospira elegans. The amount of 2mg/ml oviposition in the high concentration group was higher than that in the low concentration group and blank control group at the early stage of feeding, and the 1mg/ml oviposition quantity of the low concentration feeding group showed an increasing trend with the prolongation of feeding time. The swallowing rate of nematodes fed with water extract of pine pollen was generally higher than that of control group, which indicated that the effect of pine pollen on physiological indexes of nematodes was not caused by heat restriction. The effect of high concentration pine pollen 2mg/ml feeding nematode on nematode movement ability was less than that of blank control group. In addition, in the oxidative pressure resistance test, the nematodes fed with water extract of 1 mg / ml pine pollen had significant thermal resistance protection, and the survival rate of the low concentration pine pollen feeding group was higher than that of the high concentration feeding group. The results showed that the water extract of pine pollen could protect nematodes against oxidative damage induced by heat shock to varying degrees. 10 mm hydrogen peroxide induced oxidation pressure in vitro. The water extract of pine pollen had a good protective effect on nematodes and was in the range of low concentration. The protective effect was significantly different from that of the blank control group. The results showed that the water extract of pine pollen had a good protective effect against hydrogen peroxide induced oxidative stress damage to nematodes. To sum up, this paper takes nematodes as a model, through the evaluation of the antioxidant effect of "medicinal and food homologous" plants and the evaluation of the anti-oxidation effect of pine pollen, provides the experimental basis for the anti-oxidation and anti-senescence efficacy of "medicinal and food homologous" plants.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R285.5

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