选择性激光熔覆制备多孔钛试件及动物实验研究
发布时间：2018-09-01 13:32
【摘要】：目的本实验以纯钛粉为实验材料,通过计算机辅助设计(CAD),对钛试件表面结构进行重新设计,并通过选择性激光熔覆(SLM)技术制备出表面多孔的钛试件。通过对试件的弹性模量检测及动物实验研究,评价此结构的力学性能及对成骨活性的影响。方法1、选取直径0-45μm的二级球形钛粉,通过试件设计改良和CAD,在氩气(Ar)保护下寻找最优激光参数,进行SLM技术制备所需试件。在适当激光参数条件下,制备出表层不同孔径大小的试件。同时以传统大颗粒喷砂酸蚀(SLA)工艺制备的试件为对照组。2、使用场发射扫描电镜(FE-SEM)观察每组试件的表面形貌、孔隙直径等,并使用X射线能量色散谱仪(EDS)对试件的表面元素成分进行分析。并通过MTS 810万能电子测试机得到“应力-应变曲线”,比较不同组试件弹性模量差异。3、将三组试件植入兔股骨,在术后4周、8周和12周分别处死,通过肉眼观察、X线观察、VG染色观察骨组织愈合,骨-种植体结合率(BIC)评价微孔结构对新骨形成的影响。4、使用SPSS 16.0对实验结果进行统计学分析。结果1、大孔径组试件有明显银白色金属光泽,表面有肉眼可见的孔洞结构,电镜下孔隙近圆形,孔径范围在200-250μm之间。小孔径组试件表面金属光泽更优于大孔径组,表面未见明显孔洞结构,电镜下见孔隙范围在150-200μm之间。两组试件表面均附有未完全熔融的钛粉颗粒。SLA试件表面呈暗灰色,无金属光泽,电镜下表面呈不规则的闭孔微孔结构。2、EDS结果显示SLA试件表面的碳元素高于SLM试件。通过“应力-应变曲线”测出大孔径组试件弹性模量27.7±1.3GPa,小孔径组试件34.5±0.9GPa,SLA试件101.6±1.1GPa。3、在体内植入实验中,种植体-骨组织界面形成良好的骨结合,在试件表面孔隙中有新骨长入。小孔径组试件并未表现出比SLA试件更加优良的生物学性能,但大孔径组试件在第8周,骨种植体接触率达80.77%,相较于小孔径组的71.91%和SLA的65.22%具有明显差异(P0.05),在中期骨诱导骨形成的能力上优于另两组。结论通过软件设计,使激光在既定程序下制备出的多孔种结构试件,其弹性模量接近人体骨皮质。孔径在200-250μm之间的多孔试件具有良好的生物相容性和骨诱导作用,使骨愈合时间缩短,促进新骨形成,并能使新骨长入孔隙内。孔洞的形成降低了试件的弹性模量,较传统植体,更加接近于人体骨松质弹性模量,且大孔径钛试件弹性模量小于小孔径钛小于喷砂酸蚀试件。
[Abstract]:Aim in this experiment, the surface structure of titanium specimen was redesigned by computer aided design (CAD),) with pure titanium powder as the experimental material, and the porous titanium specimen was prepared by selective laser cladding (SLM) technology. The mechanical properties of the structure and its effect on the osteogenesis activity were evaluated by measuring the elastic modulus of the specimen and studying the animal experiment. Methods 1. The second order spherical titanium powder with the diameter of 0-45 渭 m was selected, and the optimum laser parameters were obtained by improving the design of the specimen and CAD, under the protection of argon (Ar), and the required samples were prepared by SLM technology. The samples with different pore sizes were prepared under the appropriate laser parameters. The surface morphology and pore diameter of each sample were observed by field emission scanning electron microscopy (FE-SEM). The surface elemental composition of the specimen was analyzed by using X-ray energy dispersive spectrometer (EDS). The "stress-strain curve" was obtained by MTS 810 universal electronic testing machine. The difference of elastic modulus of different groups was compared. The three groups of specimens were implanted into the femur of rabbits. The rabbits were killed at 4 weeks, 8 weeks and 12 weeks after operation, respectively. Bone healing was observed by X-ray staining and bone implant bonding rate (BIC) was used to evaluate the effect of micropore structure on the formation of new bone. SPSS 16.0 was used to analyze the experimental results. Results 1. The samples with large pore size had obvious silver-white metallic gloss, the pore structure was visible to the naked eye on the surface, the pore size was nearly circular under electron microscope, and the pore size ranged from 200 to 250 渭 m. The metallic gloss of the sample with small pore size is better than that of the large pore group. There is no obvious pore structure on the surface, and the pore size ranges from 150 渭 m to 200 渭 m under electron microscope. The surface of the samples was dark gray with incomplete melting titanium particles. The surface of the samples had no metallic gloss. Under the electron microscope, the surface of the samples showed irregular closed pore micropore structure. The results showed that the carbon element on the surface of the SLA specimen was higher than that of the SLM specimen. The elastic modulus of large pore group specimen was 27.7 卤1.3 GPA, and that of small pore group specimen was 34.5 卤0.9 GPaSLA-SLA-sample 101.6 卤1.1 GPa.3.The implant bone interface formed good bone bonding in vivo, and new bone was formed in the surface pore of the specimen. The results showed that the elastic modulus of the specimen was 27.7 卤1.3 GPA, and that of the small pore size group was 34.5 卤0.9 GPaSLA-SLA specimen 101.6 卤1.1 GPa.3.The implant bone interface formed a good bone bonding in vivo. The biological properties of small pore group specimens were not better than those of SLA specimens, but the large pore size specimens showed no biological properties at the 8th week. The contact rate of bone implant reached 80.77%, which was significantly higher than that of small aperture group (71.91%) and SLA (65.22%) (P0.05). Conclusion through the software design, the elastic modulus of the porous specimen prepared by laser under the established procedure is close to that of human bone cortex. The porous specimen with a pore diameter of 200-250 渭 m has good biocompatibility and bone induction, shortens the bone healing time, promotes the formation of new bone, and makes the new bone grow into the pore. The formation of the pore decreases the elastic modulus of the specimen, which is closer to the elastic modulus of the human bone cancellous than the traditional implant, and the elastic modulus of the large aperture titanium specimen is smaller than that of the small pore diameter titanium specimen than that of the sand blast acid etching specimen.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.1
本文编号：2217358
[Abstract]:Aim in this experiment, the surface structure of titanium specimen was redesigned by computer aided design (CAD),) with pure titanium powder as the experimental material, and the porous titanium specimen was prepared by selective laser cladding (SLM) technology. The mechanical properties of the structure and its effect on the osteogenesis activity were evaluated by measuring the elastic modulus of the specimen and studying the animal experiment. Methods 1. The second order spherical titanium powder with the diameter of 0-45 渭 m was selected, and the optimum laser parameters were obtained by improving the design of the specimen and CAD, under the protection of argon (Ar), and the required samples were prepared by SLM technology. The samples with different pore sizes were prepared under the appropriate laser parameters. The surface morphology and pore diameter of each sample were observed by field emission scanning electron microscopy (FE-SEM). The surface elemental composition of the specimen was analyzed by using X-ray energy dispersive spectrometer (EDS). The "stress-strain curve" was obtained by MTS 810 universal electronic testing machine. The difference of elastic modulus of different groups was compared. The three groups of specimens were implanted into the femur of rabbits. The rabbits were killed at 4 weeks, 8 weeks and 12 weeks after operation, respectively. Bone healing was observed by X-ray staining and bone implant bonding rate (BIC) was used to evaluate the effect of micropore structure on the formation of new bone. SPSS 16.0 was used to analyze the experimental results. Results 1. The samples with large pore size had obvious silver-white metallic gloss, the pore structure was visible to the naked eye on the surface, the pore size was nearly circular under electron microscope, and the pore size ranged from 200 to 250 渭 m. The metallic gloss of the sample with small pore size is better than that of the large pore group. There is no obvious pore structure on the surface, and the pore size ranges from 150 渭 m to 200 渭 m under electron microscope. The surface of the samples was dark gray with incomplete melting titanium particles. The surface of the samples had no metallic gloss. Under the electron microscope, the surface of the samples showed irregular closed pore micropore structure. The results showed that the carbon element on the surface of the SLA specimen was higher than that of the SLM specimen. The elastic modulus of large pore group specimen was 27.7 卤1.3 GPA, and that of small pore group specimen was 34.5 卤0.9 GPaSLA-SLA-sample 101.6 卤1.1 GPa.3.The implant bone interface formed good bone bonding in vivo, and new bone was formed in the surface pore of the specimen. The results showed that the elastic modulus of the specimen was 27.7 卤1.3 GPA, and that of the small pore size group was 34.5 卤0.9 GPaSLA-SLA specimen 101.6 卤1.1 GPa.3.The implant bone interface formed a good bone bonding in vivo. The biological properties of small pore group specimens were not better than those of SLA specimens, but the large pore size specimens showed no biological properties at the 8th week. The contact rate of bone implant reached 80.77%, which was significantly higher than that of small aperture group (71.91%) and SLA (65.22%) (P0.05). Conclusion through the software design, the elastic modulus of the porous specimen prepared by laser under the established procedure is close to that of human bone cortex. The porous specimen with a pore diameter of 200-250 渭 m has good biocompatibility and bone induction, shortens the bone healing time, promotes the formation of new bone, and makes the new bone grow into the pore. The formation of the pore decreases the elastic modulus of the specimen, which is closer to the elastic modulus of the human bone cancellous than the traditional implant, and the elastic modulus of the large aperture titanium specimen is smaller than that of the small pore diameter titanium specimen than that of the sand blast acid etching specimen.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.1
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