脂多糖对人肝内胆管上皮细胞IL-6/STAT3信号通路的影响及意义

发布时间：2023-01-07 09:28

　　目的研究脂多糖（LPS）刺激能否诱导正常人肝内胆管上皮细胞（HIBECs）白细胞介素-6（IL-6）/信号转导与转录激活因子3（STAT3）信号通路的激活及对该信号通路下游因子c-Myc和髓样细胞白血病-1（Mcl-1）表达的影响;探讨LPS对HIBECs细胞增殖的影响及其可能存在的机制。方法（1）HIBECs细胞常规体外培养,使用不同浓度的LPS（0、0.1、1、2、4、8μg/ml）分别刺激该细胞,并分别作用24h、48h、72h,酶联免疫吸附实验（ELISA）检测各浓度、各时间点IL-6的表达水平,确定LPS的最适刺激浓度及最佳处理时间,再以其进行后续实验;（2）用前述所测LPS刺激HIBECs细胞,并设立正常细胞对照组,Western blot检测磷酸化的STAT3（p-STAT3）蛋白和STAT3蛋白表达情况,确定pSTAT3/STAT3蛋白比例,探讨LPS能否激活IL-6/STAT3信号通路;（3）再以前述所测LPS干预HIBECs细胞,同时设立正常细胞对照组,分别采用荧光定量逆转录PCR（RT-q PCR）和Western blot测定c-Myc、Mcl-1的m RNA及... 

【文章页数】：50 页

【学位级别】：硕士

【文章目录】：
英文缩略词表
中文摘要
英文摘要
1.前言
2.材料与方法
3.结果
4.讨论
5.结论
参考文献
附录
致谢
综述
    参考文献


【参考文献】：
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