补光时间及光质对温室黄瓜幼苗生长及光合特性的影响

发布时间：2018-04-26 07:18

  本文选题：LED + 黄瓜　； 参考：《甘肃农业大学》2016年博士论文

【摘要】：为了研究补光时间与光质对黄瓜(Cucumis sativus L.)幼苗生长和光合特性的影响的影响,以不补光为对照,分析了补光时间(2h,4h和8h)及光质[红蓝复合光(R:B=7:3,R:B=8:2)、蓝光(B)、红光(R)和白光(W)]对黄瓜幼苗形态学指标、根系活力、抗氧化酶系统、叶绿素荧光参数、光合速率等参数的影响,主要研究结果如下:(1)补照4h的红蓝复合光(7R/3B)有利于黄瓜幼苗地上部分的生长及干物质的积累。补照4h的红蓝复合光(7R/3B)能有效促进了黄瓜幼苗植株地上部分纵向生长和茎粗的增加。随着补光处理时间的延长,补光时间及光质对黄瓜幼苗叶面积生长的促进作用逐渐显现,补光4h处理及补照红蓝复合光对黄瓜幼苗叶面积及地上部干物重的促进效果最为明显。(2)补光时间及光质对黄瓜幼苗根长、根表面积、根分枝数、根系活力及壮苗指数影响显著,且互作效应显著。补充4h红蓝复合光(7:3)可显著提高根系活力,进而有效促进了黄瓜幼苗干物质的积累,为培育壮苗奠定了生理基础。补光4h处理黄瓜各项生长指标分别优于补光2h、8h和不补光处理;补照红蓝复合光处理黄瓜各项生长指标分别优于蓝光、红光、白光和不补光处理。其中,4h补照7R/3B处理下黄瓜幼苗根长、根表面积、根分枝数和根系活力最高,分别达到1028.0 cm/株、124.1 cm-2/株、5165.3个/株和481.9 mg g-1 h-1;黄瓜幼苗根干重达到0.072g株-1,显著高于补光2h蓝、红、白光和补光8h蓝、红、白光处理及对照。黄瓜幼苗地上部干重、根干重均与根系活力呈显著的正相关关系(P0.01)。(3)补照4h蓝光可以明显提高黄瓜幼苗叶片可溶性蛋白的含量及保护酶(POD、SOD、APX和CAT)活性,并且使MDA的含量保持相对较低的水平,从而可以有效延缓叶片衰老。与对照相比,补光可显著提高黄瓜幼苗叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,其中以补光4h红蓝复合光处理(7R/3B和8R/2B)效果最为显著;补照不同光质可提高黄瓜幼苗可溶性蛋白的含量,其中,以蓝光效果最好。(4)补光时间与光质对黄瓜幼苗叶片叶绿荧光参数的影响均明显表现出处理时间的累加效应。红蓝复合光处理条件下,黄瓜幼苗叶片叶绿素Fv/Fm、ΦPSII和qP值均高于蓝光、红光处理和对照,而NPQ最低。补光时间与光质对黄瓜幼苗叶片净光合速率影响主要体现在一日内的10:00至16:00,峰值均出现在12:00。适宜的补光时间及光质组合通过提高黄瓜幼苗叶片叶绿素含量和根系活力,增加叶面积,从而提高黄瓜幼苗叶片净光合速率。(5)不同补光时间及光质对黄瓜幼苗叶片SPAD存在显著影响,且互作效应显著。补光时间与光质对黄瓜幼苗叶片SPAD的影响存在时间上的叠加效应;不同补光时间与光质处理条件下,黄瓜幼苗叶色的变化可以表征叶绿素含量的变化。延长光照时间和补照红蓝复合光、蓝光、红光和白光在一定程度上提高了黄瓜叶片SPAD值。补光时间和光质对黄瓜幼苗叶绿体超微结构产生了明显影响;补照红光和蓝光条件下,淀粉颗粒数量增多,补照红蓝复合光叶绿体结构良好,线粒体丰富,基粒、基质片层清晰。
[Abstract]:In order to study the effect of light time and light quality on the growth and photosynthetic characteristics of Cucumber (Cucumis sativus L.) seedlings, the morphological index, root activity and antioxidant enzyme system of cucumber seedlings were analyzed by the light supplement (2h, 4H and 8h) and light quality [red blue composite light (R:B=7:3, R:B=8:2), blue light (B), red light (R) and white light (W)]. The main results were as follows: (1) the red and blue composite light (7R/3B) of 4H was beneficial to the growth of the aboveground part of the cucumber seedlings and the accumulation of dry matter. The supplementation of the red and blue light (7R/3B) of 4H could effectively promote the growth of the upper part of the cucumber seedlings and the increase of the stem diameter. The effect of light supplementation time and light quality on leaf area growth of cucumber seedlings gradually appeared with the prolongation of light treatment time. The effect of supplementing light 4H treatment and supplementing red and blue light on leaf area and dry matter weight of cucumber seedlings was the most obvious. (2) the time and light quality of the cucumber seedling root length, root surface area, root branch number and root system The vitality and seedling index were significantly affected, and the interaction effect was significant. Supplementing 4H red and blue light (7:3) could significantly increase the root activity, and thus effectively promote the accumulation of dry matter in cucumber seedlings, and lay a physiological basis for the cultivation of the strong seedlings. The growth index of the supplemental 4h treatment of cucumber is superior to the supplement 2h, 8h and non light treatment, and the combination of red and blue and blue. The growth indexes of light treated cucumber were superior to blue light, red light, white light and non light treatment. Among them, the root length, root surface area, root branch number and root vitality of Cucumber Seedlings under 4H supplementation 7R/3B were the highest, which reached 1028 cm/, 124.1 cm-2/, 5165.3 / 481.9 mg g-1 H-1, and the root dry weight of cucumber seedlings reached -1 of 0.072g strain. Higher than 2H blue, red, white and light 8h blue, red and white light treatment and control. The dry weight and root dry weight of cucumber seedlings have significant positive correlation with root activity (P0.01). (3) the content of soluble protein in cucumber leaves and the activity of protective enzymes (POD, SOD, APX and CAT) in cucumber seedlings can be significantly improved, and the content of MDA is guaranteed. Compared with the control, the content of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content were significantly increased in the leaves of cucumber seedlings compared with the control. The effect of supplementing light 4H red blue light treatment (7R/3B and 8R/2B) was the most significant, and different light quality could improve the soluble egg of Cucumber seedlings. The effect of blue light was the best in the content of white, and (4) the effect of the time and light quality on the chlorophyll fluorescence parameters of the leaves of cucumber seedlings showed a cumulative effect. Under the condition of red and blue light treatment, the chlorophyll Fv/Fm, PSII and qP values of cucumber seedlings were higher than those of blue light, red light treatment and control, while NPQ was the lowest. The effect of light quality on the net photosynthetic rate of cucumber seedlings was mainly reflected from 10:00 to 16:00 in one day. The peak value of the peak was in the appropriate time of 12:00. and the combination of light quality. The leaf area was increased by increasing the chlorophyll content and root activity of cucumber seedlings, and the net photosynthetic rate of the leaves of yellow melon seedlings was increased. (5) different light filling time. The effect of light quality on the leaf SPAD of cucumber seedlings was significant, and the interaction effect was significant. The effect of light time and light quality on the leaf SPAD of cucumber seedlings had a time superposition effect. Under the different light time and light quality, the change of leaf color of cucumber seedlings could represent the change of chlorophyllin content. Blue light, blue light, red light and white light increased the SPAD value of cucumber leaves to a certain extent. The time and light quality of the cucumber seedlings had an obvious influence on the ultrastructure of the chloroplasts of cucumber seedlings; the amount of starch granules increased under the conditions of red and blue light, and the structure of the red and blue compound light leaf green body was good, the mitochondria were rich, the granules and the matrix layer were clear. Clear.

【学位授予单位】：甘肃农业大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：S626;S642.2
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本文编号：1805032