猪卵母细胞成熟和早期胚胎发育过程中的microRNA表达谱研究

发布时间：2018-06-26 14:43

本文选题：猪 + 卵母细胞　；参考：《中国农业科学院》2016年博士后论文

【摘要】：猪是广泛饲养的经济动物,提供重要的肉食品来源；也是重要的实验动物,是人类医学的试验模型之一。microRNAs (miRNAs)参与如细胞增殖、分化等许多生物学过程的调控。已有的研究发现,在许多物种的卵母细胞成熟和早期胚胎发育过程中,miRNA的表达丰度都是呈现动态变化的。miRNA可影响卵母细胞成熟、早期胚胎卵裂、母源mRNA降解等过程。但是,目前对于猪卵母细胞成熟和早期胚胎发育过程中miRNA的研究还相对较少。本研究选取GV期卵母细胞、MII期卵母细胞、4-细胞胚胎和囊胚四个时间点的样本,采用solexa测序的方法对miRNA的表达谱进行系统分析,筛选出了在猪卵母细胞成熟及早期胚胎发育过程中有重要作用的miRNAs,在对差异表达的miRNAs的靶基因进行预测后,还对这些预测的靶基因进行通路分析。此外,通过Q-PCR验证solexa测序结果的同时,对关键]miRNA如mir-27、let-7i等进行进一步试验研究,为阐明猪卵母细胞成熟及早期胚胎发育过程中miRNA的调控机制奠定了基础。
[Abstract]:Pigs are widely raised economic animals and provide important sources of meat food. They are also important experimental animals. MicroRNAs (miRNAs) are involved in many biological processes such as cell proliferation and differentiation. It has been found that the abundance of miRNA expression in oocyte maturation and early embryonic development of many species is a dynamic change. MiRNA may affect oocyte maturation, early embryo cleavage, and the degradation of mother mRNA. However, there are few studies on miRNA in pig oocytes maturation and early embryonic development. In this study, the expression profiles of miRNAs were systematically analyzed by solexa sequencing from four time points of GV phase oocyte phase II oocytes, embryos and blastocysts. MiRNAss, which play an important role in the maturation and early embryonic development of porcine oocytes, were screened. After predicting the target genes of differentially expressed miRNAs, the pathway of these predicted genes was analyzed. In addition, the solexa sequencing results were verified by Q-PCR, and the key miRNAs, such as mir-27let-7i, were further studied, which laid a foundation for elucidating the regulation mechanism of miRNAs during maturation and early embryonic development of porcine oocytes.
【学位授予单位】：中国农业科学院
【学位级别】：博士后
【学位授予年份】：2016
【分类号】：S828

【参考文献】