水稻分枝调控蛋白ABR1互作蛋白的鉴定与功能分析

发布时间：2019-01-12 14:11

【摘要】：水稻分蘖是决定植株形态和产量形成的重要农艺性状之一。水稻有效分蘖一般由着生于基部非伸长节间上的分蘖芽伸长生长而来,而着生于伸长节间上的分蘖由于很难长出稻穗被认为是无效分蘖。为了避免无效分蘖对营养物质的过多消耗,探索抑制伸长节间上分蘖芽伸长生长的机制,对于改良水稻植株形态、提高产量形成潜力具有重要意义。前期的研究中,我们鉴定了一个分枝异常突变体abr1,由于伸长节间上的分蘖芽休眠被打破,从而伸长生长为分蘖,最终表现为分蘖数增多。在本研究中,我们发现ABR1调节上位分蘖芽伸长独立于独脚金内脂途径。ABR1编码一个胞质定位的蛋白,与两个同源基因K12A/B分别互作。转基因株系k12ak12b中也观察到了类似于abr1突变体的表型,表明K12A/B具有调节水稻伸长节间上分蘖芽伸长生长的功能。综合上述研究结果,初步明确了 ABR1与K12A/B蛋白相互作用调节伸长节间上分蘖芽的伸长生长。
[Abstract]:Rice tillering is one of the important agronomic traits that determine the formation of plant morphology and yield. Rice effective tillers are generally derived from the elongation of tiller buds on non-elongated internodes at the base, while tillers on elongated nodes are considered ineffective because of the difficulty in producing rice panicles. In order to avoid excessive consumption of nutrients by ineffective tillers, it is of great significance to explore the mechanism of inhibiting the growth of tiller buds at elongation Internode, which is of great significance for improving the morphology of rice plants and increasing the potential of yield formation. In previous studies, we identified a mutant with abnormal branching, abr1, which was broken by dormancy of the tiller bud at the elongation Internode, and thus grew into a tiller, which eventually showed an increase in the number of tillers. In this study, we found that ABR1 regulates the elongation of superior tiller buds independent of the monopodium internal lipid pathway. ABR1 encodes a cytoplasmic localization protein and interacts with two homologous genes, K12A/B, respectively. Phenotypes similar to those of abr1 mutants were also observed in transgenic line k12ak12b, indicating that K12A/B could regulate the growth of tiller buds on the elongation internodes of rice (Oryza sativa L.). Based on the above results, the interaction between ABR1 and K12A/B protein was used to regulate the growth of tiller buds at elongation Internode.
【学位授予单位】：中国农业科学院
【学位级别】：博士后
【学位授予年份】：2017
【分类号】：S511

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