葡萄籽提取物及龟板提取物对胫骨软骨发育不良肉鸡治疗效果的研究
发布时间：2021-09-02 00:47
胫骨软骨发育不良（TD）属于禽类代谢紊乱病,主要发生在快速生长的禽类中,特别是在肉鸡中。其特征是生长板（GP）的非矿化和非血管化,导致肉鸡跛行及软骨细胞的异常生长。据报道,TD的发病率约占家禽骨病的30%,其在中国发病率约为10%,并能导致农民的利润率严重下降。TD的临床和亚临床症状主要为免疫力降低、骨髓炎、生长缓慢、及乳腺囊肿形成。进而引起动物伦理和福利问题,降低肉鸡的胴体和肉的质量。1.葡萄籽提取物通过下调CA2基因和增强肝功能恢复受胫骨软骨发育不良影响的鸡胫骨软骨发育不良（TD）是一种严重的肉鸡代谢综合征,其表现为生长板（GP）向胫骨干骺端增生。葡萄籽提取物（GSE）是是一种天然的植物提取物,在抗氧化,抗炎、抗癌和减少机体氧化损伤方面起着重要作用。本实验旨在评估GSE对发生TD的肉鸡的治疗作用。为此,我们购买了210只雏鸡并在前3天喂食正常的初始饲料。之后,所有雏鸡分成两组,对照组为70只鸡（正常饮食）,另一组为试验组140鸡（正常饮食+福美双50mg/kg）。试验组在第7天又被平分为2组,分别为TD组和GSE组。饲喂结果显示:与TD组相比,GSE显著（P<0.05）减少了...
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：94 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
List of abbreviations
CHAPTER1 Introduction
1.1 Endochondral ossification
1.2 Bone developmental process
1.3 Growth plate structure
1.4 Avian epiphyseal plate
1.5 Tibial dyschondroplasia(TD)
1.6 TD and gene expression
1.6.1 Carbonic Anhydrase2 (CA2)
1.6.2 Hypoxia Inducible Factor1 (HIF-1α)and Hsp90
1.6.3 AKT and PI3K
1.7 Grape Seed Extract
1.8 Plastrum Testudinis Extract
1.9 Inference and hypothesis
CHAPTER2 Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract Through Downregulating CA2 Gene and Enhancing Liver Functions
2.1 Introduction and objectives of the study
2.2 Materials and Methods
2.2.1 Ethical Approval
2.2.2 Experimental Design
2.2.3 Analysis of Production Performance
2.2.4 Analysis of Blood Biochemistry
2.2.5 Analysis of Liver Antioxidants and Hematoxylin& Eosin(H&E)Staining
2.2.6 Extraction of RNA and RT-q PCR Analysis
2.2.7 Western blotting analysis
2.2.8 Immunohistochemistry
2.2.9 Statistical analysis
2.3 Results
2.3.1 Growth performance and mortality
2.3.2 Effect of GSE on TD score and growth-plate width
2.3.3 Analysis of blood biochemistry and liver histopathological inspection
2.3.4 Evaluation of liver antioxidant status
2.3.5 CA2 m RNA and protein expression in tibia growth plate
2.3.6 Immunohistochemistry of growth plates
2.4 DISCUSSION
CHAPTER3 Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence,Liver Weight,and Tibial Angiogenesis in Chickens
3.1 Introduction and objectives of the study
3.2 Materials and methods
3.2.1 Animal welfare and ethics
3.2.2 Birds raising plan
3.2.3 Morphological inspection,tibia parameters and liver weight
3.2.4 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry(IHC)
3.2.5 Real time quantitative PCR(RT-q PCR)
3.2.6 Western Blot analysis
3.2.7 Statistical analysis
3.3 RESULTS
3.3.1 Weight gain and mortality
3.3.2 Effect of GSE on TD incidence and liver weight
3.3.3 The general tibial parameters examination
3.3.4 Clinical evaluation of the TD and GSE chickens
3.3.5 Histopathological investigation of the growth plates of tibia
3.3.6 Immunohistochemistry of Tibial growth plates
3.3.7 The HIF-1αand Hsp90 m RNA and protein expression in growth plate
3.4 DISCUSSION
CHAPTER4 Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling
4.1 Introduction and objectives of the study
4.2 Materials and methods
4.2.1 Reagents and compounds
4.2.2 Approval of Ethics
4.2.3 Experimental plan
4.2.4 Analyses of Production Parameters and Tibia Parameters
4.2.5 Analysis of Blood Biochemistry
4.2.6 Liver weight and Antioxidants Analysis
4.2.7 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry
4.2.8 RNA Extraction and RT-q PCR
4.2.9 Western Blot analysis
4.2.10 Statistical analysis
4.3 RESULTS
4.3.1 Production parameters
4.3.2 Effect of PTE on growth-plate size,tibia parameters,and TD score
4.3.3 Biochemical criterion analysis of serum and liver histopathology
4.3.4 Liver weight and determination of antioxidant status in liver
4.3.5 Histopathological investigation of the growth plates of tibia
4.3.6 Immunohistochemistry of Tibial growth plates
4.3.7 AKT and PI3K genes and protein expression in growth plate
4.4 DISCUSSION
CHAPTER5 Conclusions and Future Prospects
5.4 Future Prospects
References
Acknowledgement
本文编号：3377974
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：94 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
List of abbreviations
CHAPTER1 Introduction
1.1 Endochondral ossification
1.2 Bone developmental process
1.3 Growth plate structure
1.4 Avian epiphyseal plate
1.5 Tibial dyschondroplasia(TD)
1.6 TD and gene expression
1.6.1 Carbonic Anhydrase2 (CA2)
1.6.2 Hypoxia Inducible Factor1 (HIF-1α)and Hsp90
1.6.3 AKT and PI3K
1.7 Grape Seed Extract
1.8 Plastrum Testudinis Extract
1.9 Inference and hypothesis
CHAPTER2 Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract Through Downregulating CA2 Gene and Enhancing Liver Functions
2.1 Introduction and objectives of the study
2.2 Materials and Methods
2.2.1 Ethical Approval
2.2.2 Experimental Design
2.2.3 Analysis of Production Performance
2.2.4 Analysis of Blood Biochemistry
2.2.5 Analysis of Liver Antioxidants and Hematoxylin& Eosin(H&E)Staining
2.2.6 Extraction of RNA and RT-q PCR Analysis
2.2.7 Western blotting analysis
2.2.8 Immunohistochemistry
2.2.9 Statistical analysis
2.3 Results
2.3.1 Growth performance and mortality
2.3.2 Effect of GSE on TD score and growth-plate width
2.3.3 Analysis of blood biochemistry and liver histopathological inspection
2.3.4 Evaluation of liver antioxidant status
2.3.5 CA2 m RNA and protein expression in tibia growth plate
2.3.6 Immunohistochemistry of growth plates
2.4 DISCUSSION
CHAPTER3 Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence,Liver Weight,and Tibial Angiogenesis in Chickens
3.1 Introduction and objectives of the study
3.2 Materials and methods
3.2.1 Animal welfare and ethics
3.2.2 Birds raising plan
3.2.3 Morphological inspection,tibia parameters and liver weight
3.2.4 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry(IHC)
3.2.5 Real time quantitative PCR(RT-q PCR)
3.2.6 Western Blot analysis
3.2.7 Statistical analysis
3.3 RESULTS
3.3.1 Weight gain and mortality
3.3.2 Effect of GSE on TD incidence and liver weight
3.3.3 The general tibial parameters examination
3.3.4 Clinical evaluation of the TD and GSE chickens
3.3.5 Histopathological investigation of the growth plates of tibia
3.3.6 Immunohistochemistry of Tibial growth plates
3.3.7 The HIF-1αand Hsp90 m RNA and protein expression in growth plate
3.4 DISCUSSION
CHAPTER4 Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling
4.1 Introduction and objectives of the study
4.2 Materials and methods
4.2.1 Reagents and compounds
4.2.2 Approval of Ethics
4.2.3 Experimental plan
4.2.4 Analyses of Production Parameters and Tibia Parameters
4.2.5 Analysis of Blood Biochemistry
4.2.6 Liver weight and Antioxidants Analysis
4.2.7 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry
4.2.8 RNA Extraction and RT-q PCR
4.2.9 Western Blot analysis
4.2.10 Statistical analysis
4.3 RESULTS
4.3.1 Production parameters
4.3.2 Effect of PTE on growth-plate size,tibia parameters,and TD score
4.3.3 Biochemical criterion analysis of serum and liver histopathology
4.3.4 Liver weight and determination of antioxidant status in liver
4.3.5 Histopathological investigation of the growth plates of tibia
4.3.6 Immunohistochemistry of Tibial growth plates
4.3.7 AKT and PI3K genes and protein expression in growth plate
4.4 DISCUSSION
CHAPTER5 Conclusions and Future Prospects
5.4 Future Prospects
References
Acknowledgement
本文编号：3377974
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