利用分子生物学方法研究葡萄非生物胁迫（干旱和铜胁迫）耐受机制和细胞自噬响应基因
发布时间：2021-09-27 20:20
葡萄是一种广泛栽植的重要果树,其生长发育易受世界范围内非生物和生物胁迫的影响。干旱胁迫和铜胁迫是两种重要的非生物胁迫,也是葡萄栽培生产中的主要限制因素。因此,有必要去挖掘能够抵御或者适应这些逆境的遗传资源。干旱胁迫下葡萄转录水平的测定:对旱干胁迫处理组和对照组构建的cDNA文库进行RNA测序（RNA-seq）分析,结果显示,在发现的12451个差异表达的基因（DEGs）中,有8021个基因上调表达,4430个基因表达下调。干旱胁迫下生理指标的测定:叶片气孔导度的下降抑制了光合活性和CO2同化作用,这与转录组分析结果一致。活性氧系统（包括应激酶及其相关蛋白）和次生代谢反应被激活,以适应干旱胁迫。同时,葡萄中脱落酸（ABA）、赤霉素（GA）、生长素（IAA）、油菜素内酯（BR）等激素也会参与到葡萄应激干旱胁迫的过程中。干旱条件下胁迫应答信号网络的构建:干旱胁迫抑制了光合作用过程,影响了干旱应答胁迫葡萄叶片中还原糖（葡萄糖和果糖）的合成。葡萄叶片内氧化应激标记物（丙二醛,MDA）引起了超氧自由基（02-）和过氧化氢（H2O2）的升高,植物体抗氧化清除系统激活以对抗活性氧（ROS）。钙离子（C...
【文章来源】：南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】：154 页
【学位级别】：博士
【文章目录】：
ABSTRACT
摘要
LIST OF ABBREVIATIONS
CHAPTER 1 Introduction and Review of Literature
1. Background
2. Overview of drought and Cu stress: plant responses and mechanism of acclimation
3. Physiological responses
3.1 Osmotic adjustment
3.2 Stomatal signaling
3.3 Reactive oxygen species (ROS) signaling
3.4 Plant hormone signaling
3.5 Mitogen-activated protein kinase (MAPK) signaling
3.6 Role of transcription factors (TFs) in abiotic stress tolerance
3.7 Role of autophagy in abiotic stress tolerance
4 Implication of Omics approaches in abiotic stress
4.1 Transcriptomics
4.2 Metabolomics
5.Grapevine in a changing environment
5.1 Drought stress
5.2 Cu Stress
6.Objectives of the study
References
CHAPTER 2 Insights into grapevine defense response against drought as revealed bybiochemical, physiological and RNA-seq analysis
1. Introduction
2 Materials and Methods
2.1 Plant material and drought treatments
2.2 Determination of important biochemistry and physiology-related traits
2.3 RNA extraction, cDNA library construction and Illumina deep sequencing
2.4 Analysis of gene expression level, gene ontology (GO) and Kyotoencyclopedia of genes and genomics (KEGG)
2.5 Illumina RNA-seq results validation by qRT-PCR
3. Results
3.1. Gene ontology (GO) and KEGG analysis of differentially expressed genes
3.2. Chlorophyll degradation and photosynthetic competencies under droughtstress
3.3 ROS system under drought stress
3.4 Plant hormone signal transduction pathway under drought stress
3.5 Proline metabolism under drought stress
3.6 Biosynthesis of secondary metabolites under drought stress
3.7 Heat shock protein (HSP) and pathogenesis-related protein (PR) in response todrought stress
3.8 qRT-PCR validation of DEGs from Illumina RNA-Seq
4 Discussion
5. Conclusion
References
CHAPTER 3 Grapevine immune signaling network in response to drought stress asrevealed by transcriptomic analysis
1. Introduction
2. Materials and Methods
2.1 Plant material and drought treatment
2.2 RNA extraction,cDNA library construction and Illumina deep sequencing
2.3 Analysis of gene expression level, Gene Ontology (GO) and KyotoEncyclopedia of Gene and Genome (KEGG)
2.4 Estimation of important physiological and biochemical parameters
2.5 Illumina RNA-Seq results validation by qRT-PCR
3. Results
3.1 GO and KEGG analysis of differentially-expressed genes in response todrought stress
3.2 Effect of drought stress on physiological and biochemical parameters ofgrapevine
3.3 Calcium signaling pathway in response to drought stress
3.4. MAPK-signaling pathway in response to drought stress
3.5. Plant-pathogen interaction pathway in response to drought stress
3.6. qRT-PCR validation of differentially expressed genes from Illumina RNA-Seq
4. Discussion
5. Conclusion
References
CHAPTER 4 Genome-wide analysis of autophagy-related genes (ARGs) in grapevine andplant tolerance to copper stress
1. Introduction
2. Material and methods
2.1. Plant material
2.2 Identification of autophagy-related genes (ARGs) in grapevine
2.3 Bioinformatics analysis of autophagy-related genes (ARGs)
2.4 VvARGs expression profiles
2.5 Autophagosome monitoring and malondialdehyde measuring in grapevineleaves
2.6 RNA isolation and RT-qPCR
3. Results
3.1 Identification of 35 autophagy-related genes (ARGs) in the grape genome
3.2 family conservation and expansion of VvARG homologues
3.3 Duplication events and divergence rates of VvARG genes
3.4 Grape ARG expression profiles at various fruit developmental stages
3.5 Grape ARG gene expression profiles under abiotic stresses
3.6 Autophagy monitoring and MDA measuring in the grape leaf under copperstress
3.7 RT-qPCR of grapevine ARG genes
4 Discussion
4.1 Bioinformatics analysis of VvARG genes
4.2 Expression profile of grapevine ARGs
4.3 Copper and autophagy
5 Conclusion
References
RESEARCH OUT COMES AND FUTURE PERSPECTIVE
PUBLICATION
ACKNOWLEDGEMENTS
【参考文献】：
期刊论文
[1]适于葡萄不同组织RNA提取方法的筛选[J]. 张彦苹,王晨,于华平,蔡斌华,房经贵. 西北农业学报. 2010(11)
[2]Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development[J]. Genji Qin~1 Zhiqiang Ma~1 Li Zhang~1 Shufan Xing~1 Xianhui Hou~1 Jie Deng~1 Jingjing Liu~1 Zhangliang Chen~(1,2) Li-Jia Qu~(1,2) Hongya Gu~(1,2) ~1National Laboratory for Protein Engineering and Plant Genetic Engineering,Peking- Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology,College of Life Sciences,Peking University,Beijing 100871,China; ~2The National Plant Gene Research Center (Beijing),Beijing 100101,China. Cell Research. 2007(03)
本文编号：3410556
【文章来源】：南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】：154 页
【学位级别】：博士
【文章目录】：
ABSTRACT
摘要
LIST OF ABBREVIATIONS
CHAPTER 1 Introduction and Review of Literature
1. Background
2. Overview of drought and Cu stress: plant responses and mechanism of acclimation
3. Physiological responses
3.1 Osmotic adjustment
3.2 Stomatal signaling
3.3 Reactive oxygen species (ROS) signaling
3.4 Plant hormone signaling
3.5 Mitogen-activated protein kinase (MAPK) signaling
3.6 Role of transcription factors (TFs) in abiotic stress tolerance
3.7 Role of autophagy in abiotic stress tolerance
4 Implication of Omics approaches in abiotic stress
4.1 Transcriptomics
4.2 Metabolomics
5.Grapevine in a changing environment
5.1 Drought stress
5.2 Cu Stress
6.Objectives of the study
References
CHAPTER 2 Insights into grapevine defense response against drought as revealed bybiochemical, physiological and RNA-seq analysis
1. Introduction
2 Materials and Methods
2.1 Plant material and drought treatments
2.2 Determination of important biochemistry and physiology-related traits
2.3 RNA extraction, cDNA library construction and Illumina deep sequencing
2.4 Analysis of gene expression level, gene ontology (GO) and Kyotoencyclopedia of genes and genomics (KEGG)
2.5 Illumina RNA-seq results validation by qRT-PCR
3. Results
3.1. Gene ontology (GO) and KEGG analysis of differentially expressed genes
3.2. Chlorophyll degradation and photosynthetic competencies under droughtstress
3.3 ROS system under drought stress
3.4 Plant hormone signal transduction pathway under drought stress
3.5 Proline metabolism under drought stress
3.6 Biosynthesis of secondary metabolites under drought stress
3.7 Heat shock protein (HSP) and pathogenesis-related protein (PR) in response todrought stress
3.8 qRT-PCR validation of DEGs from Illumina RNA-Seq
4 Discussion
5. Conclusion
References
CHAPTER 3 Grapevine immune signaling network in response to drought stress asrevealed by transcriptomic analysis
1. Introduction
2. Materials and Methods
2.1 Plant material and drought treatment
2.2 RNA extraction,cDNA library construction and Illumina deep sequencing
2.3 Analysis of gene expression level, Gene Ontology (GO) and KyotoEncyclopedia of Gene and Genome (KEGG)
2.4 Estimation of important physiological and biochemical parameters
2.5 Illumina RNA-Seq results validation by qRT-PCR
3. Results
3.1 GO and KEGG analysis of differentially-expressed genes in response todrought stress
3.2 Effect of drought stress on physiological and biochemical parameters ofgrapevine
3.3 Calcium signaling pathway in response to drought stress
3.4. MAPK-signaling pathway in response to drought stress
3.5. Plant-pathogen interaction pathway in response to drought stress
3.6. qRT-PCR validation of differentially expressed genes from Illumina RNA-Seq
4. Discussion
5. Conclusion
References
CHAPTER 4 Genome-wide analysis of autophagy-related genes (ARGs) in grapevine andplant tolerance to copper stress
1. Introduction
2. Material and methods
2.1. Plant material
2.2 Identification of autophagy-related genes (ARGs) in grapevine
2.3 Bioinformatics analysis of autophagy-related genes (ARGs)
2.4 VvARGs expression profiles
2.5 Autophagosome monitoring and malondialdehyde measuring in grapevineleaves
2.6 RNA isolation and RT-qPCR
3. Results
3.1 Identification of 35 autophagy-related genes (ARGs) in the grape genome
3.2 family conservation and expansion of VvARG homologues
3.3 Duplication events and divergence rates of VvARG genes
3.4 Grape ARG expression profiles at various fruit developmental stages
3.5 Grape ARG gene expression profiles under abiotic stresses
3.6 Autophagy monitoring and MDA measuring in the grape leaf under copperstress
3.7 RT-qPCR of grapevine ARG genes
4 Discussion
4.1 Bioinformatics analysis of VvARG genes
4.2 Expression profile of grapevine ARGs
4.3 Copper and autophagy
5 Conclusion
References
RESEARCH OUT COMES AND FUTURE PERSPECTIVE
PUBLICATION
ACKNOWLEDGEMENTS
【参考文献】：
期刊论文
[1]适于葡萄不同组织RNA提取方法的筛选[J]. 张彦苹,王晨,于华平,蔡斌华,房经贵. 西北农业学报. 2010(11)
[2]Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development[J]. Genji Qin~1 Zhiqiang Ma~1 Li Zhang~1 Shufan Xing~1 Xianhui Hou~1 Jie Deng~1 Jingjing Liu~1 Zhangliang Chen~(1,2) Li-Jia Qu~(1,2) Hongya Gu~(1,2) ~1National Laboratory for Protein Engineering and Plant Genetic Engineering,Peking- Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology,College of Life Sciences,Peking University,Beijing 100871,China; ~2The National Plant Gene Research Center (Beijing),Beijing 100101,China. Cell Research. 2007(03)
本文编号：3410556
本文链接：https://www.wllwen.com/shoufeilunwen/nykjbs/3410556.html
最近更新
教材专著
