大豆疫霉RxLR效应分子Avh52的功能与作用机制研究
发布时间:2021-11-26 18:17
大豆疫霉(Phytophthora sojae)引起的大豆疫霉根腐病,是大豆生产中的一种毁灭性病害,平均每年给全世界大豆生产造成10-20亿美元的经济损失。大豆疫霉在侵染大豆的过程中会分泌大约400个RxLR(Arg-any amino acid-Leu-Arg)效应分子,这些效应分子在病原菌侵染寄主的过程中发挥着重要的作用。有些效应分子能够诱发植物产生免疫反应,有些效应分子则会抑制植物产生的免疫反应,这些效应分子程序性表达、协同作用帮助病原菌致病。基于本实验室前期的研究发现大豆疫霉菌分泌的RxLR效应分子PsAvh52能够抑制PMAPs(INF1和XEG1)和效应分子(PsAvh238和PsAvh241)引起的细胞坏死。本研究在此基础上对PsAvh52的毒性功能作了进一步的探索,鉴定到了其在寄主中的靶标蛋白GmTAP1,揭示了 PsAvh52促进大豆疫霉侵染的分子机制。大豆疫霉RxLR效应分子PsAvh52毒性功能分析。本实验研究发现在大豆疫霉侵染前期,分泌的一个高表达的RxLR效应分子PsAvh52,是大豆疫霉全毒力必需的效应分子。PsAvh52在四个代表大豆疫霉生理小种的菌株间存...
【文章来源】:南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】:143 页
【学位级别】:博士
【部分图文】:
图1-2植物免疫系统模式图??Figure?1-2?Schematic?of?the?plant?immune?system.?(Dangl?et?al.?2013)??
4?2!?%??細内*—?OCN5^AOA2?g?.??Svkx^sars0?mA21?<5??G£NSi;|?C??PSR,?一^?V?访??Cytoplasm??、、'?、?…"一:―一^一一:-?.??'■????,?.......??.?,..??X?■?■?.■-:...?■.?.?■?-??:_?■….‘■:■?■???..-V.?-??Cifmsie?Opr^t?妁??图1-3大豆疫霉效应分子及其靶标互作模型??Figure?1-3?Working?models?of?several?characterized?P.?sojae?effectors?and?their?corresponding?host??targets?summarized?based?on?the?current?knowledge?(wang?et?al.?2018)??P.?sojae?secretes?effectors?that?act?in?different?cellular?compartments.?In?the?apoplast,?XEG1?plays?dual??roles?in?plant-pathogen?interactions.?XEG1?acts?as?a?virulence?effector?under?the?protection?of?its?close??ortholog?XLP1?which?competes?for?GIP1?binding?site?to?counter?the?inhibition?of
\Acy?methyltransferase?-?CM??|?t。力.??Nuc,eos〇m.?,?&C??Chromosom.?,&???methylated?cytosine?base?H?Transcription????unmethylated?cytosine?base?/??^?histone?tail?/??Chromatin?^??/?為,?????s?Messenger?RNA?(mRNA)??microRNA?(miRNA)?^??图2-1表观遗传修饰的类型??Figure?2-1?Types?of?epigenetic?modifications?(Zhou?et?al.,2010)??(A)?Histones?can?undergo?phosphorylation?(Ph),?meth>?lation?(Me),?and?acetylation?(Ac),?among?other?chemical??modifications.?These?modifications?are?involved?in?chromatin?remodeling?and?transcriptionaJ?regulation.?(B)?DNA??molecules?are?methylated?by?the?addition?of?a?methyl?group?to?carbon?position?5?on?cjlosine?bases,?a?raiction?catalyzed?by??DNA?methyllransferase?enzymes,which?maintains?repressed?ge
【参考文献】:
期刊论文
[1]Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice[J]. Yuan Li1?,Qiong Xia1?,Hongping Kou2,Dan Wang2,Xiuyun Lin3,Ying Wu1,Chunming Xu1,Shaochen Xing3 and Bao Liu1 1Key Laboratory of Molecular Epigenetics of MOE and The Institute of Genetics & Cytology,Northeast Normal University,Changchun 130024,China 2Department of Agronomy,Jilin Agricultural University,Changchun 130118,China 3Center of Agribiotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,China. Journal of Integrative Plant Biology. 2011(10)
本文编号:3520680
【文章来源】:南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】:143 页
【学位级别】:博士
【部分图文】:
图1-2植物免疫系统模式图??Figure?1-2?Schematic?of?the?plant?immune?system.?(Dangl?et?al.?2013)??
4?2!?%??細内*—?OCN5^AOA2?g?.??Svkx^sars0?mA21?<5??G£NSi;|?C??PSR,?一^?V?访??Cytoplasm??、、'?、?…"一:―一^一一:-?.??'■????,?.......??.?,..??X?■?■?.■-:...?■.?.?■?-??:_?■….‘■:■?■???..-V.?-??Cifmsie?Opr^t?妁??图1-3大豆疫霉效应分子及其靶标互作模型??Figure?1-3?Working?models?of?several?characterized?P.?sojae?effectors?and?their?corresponding?host??targets?summarized?based?on?the?current?knowledge?(wang?et?al.?2018)??P.?sojae?secretes?effectors?that?act?in?different?cellular?compartments.?In?the?apoplast,?XEG1?plays?dual??roles?in?plant-pathogen?interactions.?XEG1?acts?as?a?virulence?effector?under?the?protection?of?its?close??ortholog?XLP1?which?competes?for?GIP1?binding?site?to?counter?the?inhibition?of
\Acy?methyltransferase?-?CM??|?t。力.??Nuc,eos〇m.?,?&C??Chromosom.?,&???methylated?cytosine?base?H?Transcription????unmethylated?cytosine?base?/??^?histone?tail?/??Chromatin?^??/?為,?????s?Messenger?RNA?(mRNA)??microRNA?(miRNA)?^??图2-1表观遗传修饰的类型??Figure?2-1?Types?of?epigenetic?modifications?(Zhou?et?al.,2010)??(A)?Histones?can?undergo?phosphorylation?(Ph),?meth>?lation?(Me),?and?acetylation?(Ac),?among?other?chemical??modifications.?These?modifications?are?involved?in?chromatin?remodeling?and?transcriptionaJ?regulation.?(B)?DNA??molecules?are?methylated?by?the?addition?of?a?methyl?group?to?carbon?position?5?on?cjlosine?bases,?a?raiction?catalyzed?by??DNA?methyllransferase?enzymes,which?maintains?repressed?ge
【参考文献】:
期刊论文
[1]Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice[J]. Yuan Li1?,Qiong Xia1?,Hongping Kou2,Dan Wang2,Xiuyun Lin3,Ying Wu1,Chunming Xu1,Shaochen Xing3 and Bao Liu1 1Key Laboratory of Molecular Epigenetics of MOE and The Institute of Genetics & Cytology,Northeast Normal University,Changchun 130024,China 2Department of Agronomy,Jilin Agricultural University,Changchun 130118,China 3Center of Agribiotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,China. Journal of Integrative Plant Biology. 2011(10)
本文编号:3520680
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