吡虫啉和多菌灵分子印迹聚合物的制备及其在水果农药残留检测中的应用

发布时间：2023-04-12 00:25

　　水果在生产过程中病虫害种类多,发生频繁。杀虫剂吡虫啉和杀菌剂多菌灵在果树上应用广泛,其大量使用会产生农药残留等系列问题,因此,农残检测对保护消费者健康和保证食品质量安全监管至关重要。样品前处理技术是农药残留检测的重要基础,随着监管力度的加大,我国以及一些发达国家,农药的最大残留量(MRLs)值不断降低,痕量农药的检测需要更高效的前处理提取技术。分子印迹聚合物(MIPs)具有特异性结合力强、热化学稳定性好、可重复利用、灵敏度高、基质干扰小等优点。本文分别以甲基丙烯酰氯和β-环糊精为功能单体,制备了两种分子印迹聚合物,并将其应用于水果中吡虫啉和多菌灵残留的检测。以吡虫啉为模板,甲基丙烯酰氯为功能单体,采用表面印迹法合成了核壳型磁性分子印迹聚合物,并对其进行了表征。表征结果表明,该磁性分子印迹聚合物具有特异质结构和良好的磁性。用拟二级动力学模型对等温结合实验进行了评估,结果显示其较好的符合Freundlich等温方程。该聚合物对吡虫啉的吸附量为5.75mg g^-1,表明其具有良好的选择性提取能力。另外,该聚合物在重复使用5次后,性能没有显著性的减弱。然后,利用该分子印迹...

【文章页数】：130 页

【学位级别】：博士

【文章目录】：
摘要
abstract
Chapter 1 Introduction
    1.1 Importance and concept
    1.2 Magnetic molecular imprinting polymers
    1.3 Mechanism of molecularly imprinted process
        1.3.1 Basic approach
        1.3.2 Major components of MIPs synthesis
            1.3.2.1 Template
            1.3.2.2 Functional monomer
            1.3.2.3 Cross-linkers
            1.3.2.4 Porogenic solvents
            1.3.2.5 Initiators
    1.4 Basic polymerization approaches
    1.5 Challenges and limitations of MIPs application in pesticide detection
    1.6 Perceptive Imprinting strategies
        1.6.1 Innovative technologies for MIPs
            1.6.1.1 Nanoimprinting technology
            1.6.1.2 Living/controlled radical polymerization technology
            1.6.1.3 Hollow porous polymer synthesis technology
            1.6.1.4 Click chemistry cycloaddition reaction technology
            1.6.1.5 Microfluidic on-line synthesis technology
        1.6.2 Special Imprinting strategies
            1.6.2.1 Multi-template imprinting strategy
            1.6.2.2 Multi-functional monomer imprinting strategy
            1.6.2.3 Dummy imprinting strategy
            1.6.2.4 Segment imprinting strategy
            1.6.2.5 Composite imprinting material strategy
        1.6.3 Stimuli-responsive Imprinting Technologies
            1.6.3.1 Photo-responsive technology
            1.6.3.2 p H-Responsive technology
            1.6.3.3 Dual/multi responsive technology
            1.6.3.4 Other responsive technologies
    1.7 Physicochemical characterization of MIPs
    1.8 Application of MIPs in pesticides residues detection
        1.8.1 Organophosphorus pesticides(OPPs)
        1.8.2 Triazines
        1.8.3 Carbamates
        1.8.4 Sulphonylurea
        1.8.5 Other pesticides
Chapter 2 Synthesis of core-shell magnetic molecular imprinted polymer for the selective determination of imidacloprid in apple samples
    2.1 Introduction
    2.2 Materials and methods
        2.2.1 Reagents and chemicals
        2.2.2 Instrumentation
        2.2.3 Preparation of imprinted polymers
            2.2.3.1 Synthesis of the magnetic nanoparticles
            2.2.3.2 Modification of the magnetic nanoparticles
            2.2.3.3 Synthesis of the magnetic molecular imprinted polymers
            2.2.3.5 Kinetic adsorption procedure
            2.2.3.6 Isothermal binding procedure
            2.2.3.7 Selectivity of the polymers
            2.2.3.8 Reusability
            2.2.3.9 Selective extraction of imidacloprid from apple samples
    2.3 Results and discussion
        2.3.1 Characterization of the imprinted polymers
            2.3.1.1 Kinetic adsorption tests
            2.3.1.2 Isothermal binding test
            2.3.1.3 Selectivity and reusability
            2.3.1.4 Applicability of the developed method
Chapter 3 Selective determination of fungicide carbendazim in fruits using β-cyclodextrin based molecularly imprinted polymers
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Chemicals and reagents
        3.2.2 Instrumentation and Chromatographic analysis
        3.2.3 Preparation of molecularly imprinted polymers
        3.2.4 Kinetic adsorption kinetic test
        3.2.5 Adsorption isotherm test
        3.2.6 Selectivity binding test
        3.2.7 Reusability
        3.2.8 Determination of CBZ in fruit samples
    3.3 Results and discussion
        3.3.1 Synthesis and characterization of the molecularly imprinted polymers
        3.3.2 Binding properties of the imprinted polymers
        3.3.3 Selective adsorption and reusability
        3.3.4 Applicability of the MIPs to real fruit samples
Conclusions
References
Appendices
Acknowledgments
Author resume



本文编号：3790051