有机染料罗丹明B荧光的多功能传感研究及传感器研发

发布时间：2018-01-20 10:29

  本文关键词： 荧光传感 罗丹明B 频移 谱带重心 强度比　出处：《南昌航空大学》2017年硕士论文　论文类型：学位论文

【摘要】：罗丹明B(RhB)染料属于典型的有机发光材料,具有摩尔吸光系数高、光稳定性好、波长范围宽以及量子产率高等优点,其荧光在可见光波段便于探测,同时具有着很高的发光效率和优异的生物相容性,基于RhB荧光的传感方法及器件可以满足在不同的生理、自然环境特定的测试需求。本文对罗丹明B未作任何改性处理,对由RhB或RhB/H610胶粘剂复合材料制作的荧光探头的荧光光谱受温度、应力以及pH值等物理或化学量的影响进行了多功能传感特性研究、传感器的设计研发。本文对传统的荧光传感方法进行了归纳比较,对环境参数影响荧光的实验数据的分析主要采用谱峰频移及新型表征方法“谱带重心频移法”、“自参考双波长强度比法”和“积分强度比法”等方法。实验中采用中心波长405nm的蓝紫色激光器作为激励光源,通过设计不同的荧光传感探头和搭建相应的荧光传感系统,分别对罗丹明B受温度、应力以及pH值影响的荧光特征谱进行了全面的研究,实验总结出了罗丹明B荧光谱带重心λB、自参考双波长强度比R、积分强度比RI等荧光特征信号传感温度T(单位℃)、压应力σ(单位MPa)以及pH值的多个不同的传感方程,对荧光光纤传感器重要的静态指标(重复性、迟滞特性等)进行了分析,最后对工程化应用作了初步尝试。其中,主要实验结论是:(1)荧光传感温度:得到温度传感方程λB=589.2-0.049T,以及R=0.114+0.00204T(R=I(530nm)/I(580nm))。重心法传感系统的重复性为0.8%FS,自参考双波长强度比法传感系统的重复性为1.6%FS,二者均不存在明显的迟滞现象。(2)荧光传感应力:得到应力传感方程λB=620.89-1.08σ+0.1σ2,以及R=0.82-0.01σ(R=I(625nm)/I(615nm))。重心法传感系统的重复性为2.8%FS,自参考双波长强度比法传感系统的重复性为2.1%FS。在RhB/H610胶粘剂复合材料谱带重心波长的迟滞特性实验中观察到最大迟滞,约为11.8%FS。(3)荧光传感pH:得到pH传感方程λB=836.36-75.71pH+5.85pH2以及R=1.86-0.15pH(R=I(595nm)/I(585nm)),RI=0.17+0.04pH。(4)结合研发的罗丹明B荧光温度传感系统和荧光pH值传感系统,分别设计了微波环境中的水温监测和甘氨酸浓度测定实验,验证了系统的实用性。两种新型的传感方法(谱带重心法和自参考双波长强度比法)能够显著提高荧光传感的精度和稳定性。实验设计的荧光多功能传感系统适用于微波医学和强电磁干扰下的传感测量。
[Abstract]:Rhodamine Bu RhB) dye is a typical organic luminescent material with high molar absorptivity, good light stability, wide wavelength range and high quantum yield. At the same time, it has high luminescence efficiency and excellent biocompatibility. The sensing methods and devices based on RhB fluorescence can meet different physiological conditions. In this paper, Rhodamine B has not been modified, and the fluorescence spectrum of fluorescent probe made of RhB or RhB/H610 adhesive composite material is subjected to the temperature. The effects of physical or chemical quantities such as stress and pH value on the multifunctional sensing characteristics and the design and development of the sensor were studied. In this paper the traditional fluorescent sensing methods were summarized and compared. The spectrum peak frequency shift and a new characterization method, "spectral barycenter shift method", are mainly used to analyze the experimental data of environmental parameters affecting fluorescence. The self-reference dual-wavelength intensity ratio method and the integrated intensity ratio method are used in the experiment. A blue-purple laser with a central wavelength of 405nm is used as the exciting light source in the experiment. By designing different fluorescence sensors and setting up the corresponding fluorescence sensing system, the fluorescence characteristic spectra of Rhodamine B affected by temperature, stress and pH value were studied comprehensively. Experimental results show that the center of gravity 位 B of Rhodamine B fluorescence spectrum band, the self-reference dual-wavelength intensity ratio R, the integral intensity ratio RI and other fluorescence characteristic signal sensing temperature T (unit 鈩，

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