以泡沫铜为基底构建的纳米材料及其用于无酶葡萄糖传感器的研究

发布时间：2018-02-28 08:08

  本文关键词： 泡沫铜 葡萄糖 无酶葡萄糖传感器 分级结构 纳米管　出处：《重庆师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：葡萄糖含量的分析检测在临床医学,食品监测和生物科学等有着重要的作用。在临床医学上,葡萄糖含量的检测主要还是依靠于基于葡萄糖氧化酶(GOx)的生物传感器,但是酶是一种蛋白质,其易受外界环境(如温度,pH)的影响而失活,因此基于电极表面直接电氧化的无酶葡萄糖传感器成为现今的研究热点。过渡金属铜是构建无酶葡萄糖传感器的一种理想金属,而多孔的泡沫铜由于其具有较大的比表面积显示出了更大的研究潜能。随着近年来纳米技术的不断发展,纳米尺寸的铜及铜的化合物引起了广泛研究,将纳米材料生长在三维多孔泡沫铜基底上,多孔基底增大活性面积,促进电极表面的电子传递,结合纳米结构材料的良好物理化学性质,进一步提高了该类葡萄糖传感器的灵敏度。本论文利用XRD、SEM、EDX等物理手段对三种材料进行了微观的形貌及结构的分析,并采用循环伏安法(CV)和计时电流法(I-t)对材料的电化学性质进行表征,并将三种材料用于人体血清中葡萄糖含量的检测。本文主要研究的内容如下:(1)使用商业的泡沫铜,不需要任何预处理,直接用作工作电极进行无酶葡萄糖传感器的构建,该传感器的线性范围为1μM-0.5 mM,灵敏度为5.85mA·mM~(-1)·cm~(-2),检出限为0.5μM(S/N=3);(2)采用湿化学合成法,化学还原及电化学阳极处理的三步合成法制备了生长在泡沫铜上的铜-氢氧化铜纳米分级结构,该纳米材料在碱性条件下对葡萄糖氧化具有较高的催化活性,可用作电极材料构建无酶葡萄糖传感器的构建,在电压为0.5 V时,该传感器具有宽的线性范围为1μM~(-1) mM,高的灵敏度为9.18mA·mM~(-1)·cm~(-2)及低检出限为0.45μM(S/N=3);(3)采用自牺牲模板法制备生长在泡沫铜上的CuS纳米管,将该材料作为电极用于葡萄糖的电催化氧化,该材料表现出了良好的催化活性,在低的电压0.45 V时,在0.1 M NaOH溶液中,具有两个宽的线性范围,在1μM-0.61 mM间,灵敏度为8.66 mA·mM~(-1)·cm~(-2),在0.61 mM~(-2).41 mM间,灵敏度为2.42 mA·mM~(-1)·cm~(-2),且具有一个低的检出限为1μM(S/N=3)。
[Abstract]:Detection and analysis of the content of glucose in clinical medicine, plays an important role in food monitoring and biological science. In clinical medicine, mainly rely on the detection of glucose content based on glucose oxidase (GOx) biosensor, but the enzyme is a protein, its susceptibility to outside environment (such as temperature, pH) effect therefore, the inactivation of enzyme free glucose sensor has become a research hotspot of direct oxidation of the electrode surface. Based on the power transition metal copper is to build an ideal metal Enzymeless glucose sensor, and the porous copper foam because of its larger surface area showed greater potential for research. With the continuous development in recent years. Nanotechnology, copper and copper compounds of nanometer size has aroused extensive research, the growth of nano material in porous copper foam substrate, a porous substrate increases the active area of the electrode surface, and promote the The electron transfer, with good physical and chemical properties of nanostructured materials, to further improve the sensitivity of the glucose sensor. This paper uses XRD, SEM, EDX and other physical means to analyze the morphology and structure of three kinds of micro materials, using cyclic voltammetry (CV) and chronoamperometry (I-t) were used to characterize the electrochemical properties of materials, and three kinds of materials for the detection of glucose concentration in human serum. The contents of this paper are as follows: (1) the use of commercial copper foam, without any pretreatment, directly used as the working electrode to construct non enzymatic glucose sensor, the linear range of the sensor for 1 M-0.5 mM, the sensitivity is 5.85mA (-1) mM~ - cm~ (-2), the detection limit was 0.5 M (S/N=3); (2) using wet chemical synthesis, chemical reduction and electrochemical anodic treatment of three step synthesis of growth in copper foam 鐨勯摐-姘㈡哀鍖栭摐绾崇背鍒嗙骇缁撴瀯,璇ョ撼绫虫潗鏂欏湪纰辨，

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