干旱胁迫下外源ABA对鼓粒期大豆氮素积累的调控研究

发布时间：2018-12-24 10:43

【摘要】：本试验于2015-2016年在黑龙江八一农垦大学进行,以绥农26(干旱敏感型品种)和抗线虫9号(耐旱型品种)为供试品种,利用15N示踪技术和PEG模拟干旱胁迫的方法,研究了外源ABA对鼓粒期间干旱胁迫条件下大豆氮素积累的调控效应。试验结果表明:干旱胁迫显著降低了鼓粒期间两个供试大豆品种氮素同化和转移关键酶活性,外源ABA可缓解干旱胁迫的这种抑制作用,其中外源ABA可显著提高鼓粒前中期(鼓粒后第7 d-14 d和第14 d-21 d)绥农26NR、GS、GOGAT及GDH酶活性,对于抗线虫9号则在鼓粒前期(鼓粒后第0 d-7 d和第7 d-14 d)提高的较为显著。外源ABA提高了两个品种叶片及根部的GOT和GPT酶活性,差异未达到显著水平。干旱胁迫显著降低了鼓粒期间两个供试大豆品种植株酰脲含量、提高了氨态氮和硝态氮含量,外源ABA可调控干旱胁迫下大豆植株酰脲含量、氨态氮和硝态氮含量的趋于正常水平的作用,并且这种调控作用效果对于两个品种在鼓粒前期更为显著。干旱胁迫显著降低了鼓粒期间两个供试大豆品种植株氮素积累量,在鼓粒前期(鼓粒后第0 d-7 d和第7 d-14 d)达到显著水平。外源ABA可缓解干旱胁迫对大豆植株氮素积累(主要是矿质氮)的抑制作用,并且这种缓解作用在鼓粒前期更为显著。与干旱胁迫相比,外源ABA提高了鼓粒后第0 d-7 d和第7 d-14 d期间绥农26和抗线虫9号全株肥料氮素积累量,提高幅度分别为4.20%和3.89%、6.84%和13.49%。外源ABA提高了鼓粒后第0 d-7 d和第7 d-14 d期间绥农26和抗线虫9号全株氮素积累量,提高幅度分别为7.87%和6.53%、5.57%和4.24%。鼓粒期间干旱胁迫显著降低了两个供试大豆品种单株产量。外源ABA可缓解由干旱胁迫造成的产量损失,并且这种缓解作用在鼓粒前期更为显著。与干旱胁迫相比,外源ABA提高了鼓粒后第0 d-7 d和第7 d-14 d期间绥农26和抗线虫9号单株产量,提高幅度分别为22.08%和24.87%、18.78%和21.22%。
[Abstract]:The experiment was conducted at Bayi Nong Reclamation University of Heilongjiang Province in 2015-2016. Suinong 26 (drought sensitive variety) and nematode resistance 9 (drought tolerant variety) were used as tested varieties. 15N tracer technique and PEG were used to simulate drought stress. The effects of exogenous ABA on nitrogen accumulation in soybean during drought stress were studied. The results showed that drought stress significantly reduced the activity of nitrogen assimilation and transfer key enzymes in two soybean varieties during grain filling. Exogenous ABA alleviated the inhibition of drought stress. Exogenous ABA could significantly increase the activity of Suinong 26NRGAT and GDH enzyme in the early and middle stage (7 d ~ 14 d and 14 d ~ 21 d after granulation). The results showed that the resistance to nematode 9 was significantly increased at the pregranulation stage (0 d-7 d and 7 d-14 d after the granulation). Exogenous ABA increased the activities of GOT and GPT in the leaves and roots of the two cultivars, but the difference was not significant. Drought stress significantly decreased the content of plant acylurea and increased the content of ammonia nitrogen and nitrate nitrogen in two soybean varieties during grain filling period. Exogenous ABA could regulate the content of plant acyl urea in soybean under drought stress. The contents of ammonia and nitrate tend to be normal, and the effect of this regulation is more significant for the two varieties in the early stage of granulation. Drought stress significantly decreased the nitrogen accumulation in the two soybean varieties during the grain filling period, and reached a significant level at the early stage (0 d-7 d and 7 d ~ 14 d after grain filling). Exogenous ABA alleviated the inhibition of nitrogen accumulation (mainly mineral nitrogen) in soybean plants under drought stress, and the effect was more significant in the early stage of granulation. Compared with drought stress, exogenous ABA increased the nitrogen accumulation of Suinong 26 and resistant nematode 9 in the period of 0 d 7 d and 7 d 14 d after grain filling, and increased by 4.20% and 3.89%, respectively. 6.84% and 13.49% respectively. Exogenous ABA increased the nitrogen accumulation of Suinong 26 and resistant nematode 9 at 0 d 7 d and 7 d 14 d after grain filling, by 7.87% and 6.53% 5.57% and 4.24%, respectively. Drought stress significantly decreased the yield per plant of two soybean varieties during grain filling. Exogenous ABA can alleviate the yield loss caused by drought stress, and this effect is more significant in the early stage of granulation. Compared with drought stress, exogenous ABA increased the yield of Suinong 26 and resistant nematode 9 in the period of 0 d 7 d and 7 d 14 d after grain filling, and the increase ranges were 22.08% and 24.87%, 18.78% and 21.22%, respectively.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S565.1

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