基于代谢组学的野大豆（Glycine soja）耐盐机理研究

发布时间：2019-07-03 14:48

【摘要】：本研究以普通型野大豆和耐盐型野大豆为实验材料,采用沙土培养的方式,比较研究了中、碱性盐胁迫下两种生态型野大豆幼苗叶片中代谢物的变化。实验通过分析普通型野大豆和耐盐型野大豆响应中、碱性盐胁迫的代谢图谱来揭示野大豆的耐盐机制。实验结果显示,在两种盐胁迫的处理下,耐盐型野大豆幼苗的生长所受到的胁迫抑制明显小于普通型野大豆。差异代谢物的图谱分析显示,两种盐胁迫处理下,普通型野大豆幼苗叶片中可溶性糖和脂肪酸含量的降低程度显著高于耐盐型野大豆。这些代谢物包括乳糖、核糖、月桂酸、棕榈酸、硬脂酸以及亚麻酸。碱性盐胁迫下,两种生态型野大豆幼苗叶片中均有特异氨基酸的积累。这些氨基酸包括缬氨酸,酪氨酸,谷氨酸,亮氨酸和异亮氨酸。碱性盐胁迫下,耐盐型野大豆幼苗叶片中大部分有机酸含量以及脯氨酸含量均明显升高。这些有机酸包括粘酸、戊二酸、半乳糖酸和脱氢抗坏血酸。普通型野大豆在响应两种盐胁迫时TCA循环增强,但在耐盐型野大豆中减弱。论文结论如下:一、盐胁迫抑制野大豆幼苗的生长,根部生长受到抑制更为显著;二、碱性盐胁迫对野大豆幼苗生长的抑制更为强烈,耐盐型野大豆表现出更强的适应能力;三、普通型野大豆的耐盐机制依赖于TCA循环的增强产生更多的ATP;耐盐型野大豆更多依靠氨基酸和有机酸的代谢调节产生更多的相容性溶质。本文基于代谢组学揭示了盐胁迫下野大豆的耐盐机制,为栽培大豆的高抗育种提供了丰富的理论基础,显示出野大豆是栽培大豆遗传改良的宝贵资源,同时预示着野大豆种质资源的开发、利用和保护具有重大的意义。
[Abstract]:In this study, the changes of the metabolites in the leaves of two ecotypes of wild soybean seedlings under alkaline salt stress were studied by means of sand culture. The salt tolerance mechanism of wild soybean was revealed by analyzing the metabolic profile of basic salt stress in the response of common wild soybean and salt tolerant wild soybean. The experimental results show that under the treatment of two salt stress, the stress inhibition of the growth of the salt-resistant wild soybean seedlings is obviously lower than that of the common wild soybean. The results showed that the content of soluble sugar and fatty acid in the leaves of common wild soybean seedlings was significantly higher than that of salt-resistant wild soybean under two salt stress treatments. These metabolites include lactose, ribose, lauric acid, palmitic acid, stearic acid, and linolenic acid. The specific amino acid accumulation in the leaves of two ecotypes of wild soybean seedlings under alkaline salt stress. These amino acids include the amino acid, the tyrosine, the glutamic acid, the leucine and the isoleucine. Under alkaline salt stress, the content of most organic acids and the content of proline in the leaf of salt-resistant wild soybean seedlings were increased obviously. These organic acids include acid, glutaric, galactotic, and dehydroascorbic acid. In response to the two salt stress, the common wild soybean was enhanced in TCA cycle, but decreased in the salt-resistant wild soybean. The results of the paper are as follows:1. The salt stress inhibits the growth of the wild soybean seedlings, and the growth of the root is more obvious; secondly, the inhibition of the growth of the wild soybean seedlings by the alkaline salt stress is stronger, and the salt-tolerant wild soybean has stronger adaptability; and thirdly, The salt tolerance mechanism of the common wild soybean is dependent on the increase of the TCA cycle to produce more ATP, and the salt tolerant wild soybean is more dependent on the metabolic regulation of the amino acid and the organic acid to produce more compatible solute. The salt-tolerant mechanism of the wild soybean under salt stress is revealed based on metabolomics, which provides a rich theoretical basis for the high-resistance breeding of the cultivated soybean, and shows that the wild soybean is a valuable resource for the genetic improvement of the cultivated soybean, and also indicates the development of the wild soybean germplasm resources. And is of great significance to use and protect.
【学位授予单位】：东北师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S565.1

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