装饰材料挥发性有机物致小鼠遗传损伤作用的研究

发布时间：2018-08-16 08:59

【摘要】：目的　研究装饰材料挥发性有机物 (VOC)对小鼠骨髓细胞和外周血淋巴细胞的遗传损伤作用。方法　选择 5 5间新装修 (装修半年内 )及 18间 3年内未装修的宾馆客房 ,分别测定其空气中苯、甲苯、二甲苯、乙酸乙酯、乙酸丁酯和甲醛浓度 ,并根据现场测得的各种有机物浓度 ,用装饰材料在染毒柜内配制接近新装修现场 5、10、2 0、4 0倍的浓度进行动物 (小鼠 )染毒 ,用单细胞凝胶电泳法(SCGE)和骨髓微核试验检测其遗传损伤效应。结果　装修半年内室内空气中苯、甲苯、二甲苯、乙酸乙酯、乙酸丁酯和甲醛平均浓度分别为 6 .5 0、3.0 0、6 .70、4 1.33、1.70、0 .14mg m3,均高于未装修的客房(0 .0 8、0 .94、1.38、0 .2 5、0 .2 5、0 .0 1mg m3) ,差异均有显著性 (P 0 .0 1)。SCGE检测发现 ,10、2 0、4 0倍浓度组VOC对小鼠染毒 15d后 ,其外周血淋巴细胞DNA断裂高于对照组 ,差异均有显著性 (P 0 .0 5 ,P 0 .0 1) ;而骨髓微核试验则在 4 0倍浓度组小鼠微核率明显高于对照组 ,差异有显著性 (P 0 .0 5 )。结论　较高浓度的装饰材料VOC可引起小鼠遗传损伤作用 ,且SCGE试验比微核试验更敏感。
[Abstract]:Objective to study the genetic damage of (VOC) on bone marrow cells and peripheral blood lymphocytes of mice. Methods the concentrations of benzene, toluene, xylene, ethyl acetate, butyl acetate and formaldehyde in the air of 55 newly decorated guesthouses (within half a year) and 18 undecorated guesthouses within 3 years were determined respectively. According to the concentration of various organic compounds measured in the field, the concentration of the decoration material in the poisoning cabinet was made up of the concentration of nearly 50 times that of the newly decorated site, and the concentration was 40 times that of the newly decorated site, and the animals (mice) were exposed to it. Single cell gel electrophoresis (SCGE) and bone marrow micronucleus test were used to detect the genetic damage. Results the average concentrations of benzene, toluene, xylene, ethyl acetate, butyl acetate and formaldehyde in indoor air within half a year after decoration were 6.50 / 3.00, 6.703 / 41.33 / 1.700 / 14mg / m ~ (3), respectively, which were higher than those of the undecorated rooms (0.08 / 0, 0.94 / 1, 1.38.02 / 50.25 1mg / m ~ 3), respectively, and the average concentrations of benzene, toluene, xylene, ethyl acetate, butyl acetate and formaldehyde in indoor air were 6.50% and 3.00% respectively. There were significant differences (P 0. 01). SCGE detection showed that the DNA breakage of peripheral blood lymphocytes in mice exposed to VOC for 15 days was higher than that in the control group (P 0. 0 5), and the difference was significant (P 0. 0 5 and P 0. 01). The micronucleus rate of mice in 40 times concentration group was significantly higher than that in control group (P < 0.05), but the micronucleus rate in bone marrow micronucleus test group was significantly higher than that in control group (P < 0.05). Conclusion higher concentration of VOC can induce genetic damage in mice, and SCGE test is more sensitive than micronucleus test.
【作者单位】：深圳市卫生监督所环境卫生监督科深圳市疾病预防控制中心环境卫生科深圳市疾病预防控制中心实验室深圳市疾病预防控制中心毒理科深圳市龙岗区布吉卫生监督所
【分类号】：X503

【相似文献】