两种肾阳虚证支气管哮喘病证结合动物模型的建立与评价

发布时间：2017-12-27 03:04

本文关键词：两种肾阳虚证支气管哮喘病证结合动物模型的建立与评价　出处：《中医杂志》2016年23期 　论文类型：期刊论文

【摘要】：目的评价两种肾阳虚证支气管哮喘病证结合动物模型造模方法。方法将54只Wistar大鼠分为空白组、哮喘组、激素组、激素+哮喘组、手术组、手术+哮喘组,每组9只。激素组、激素+哮喘组大鼠采用氢化可的松注射液15 mg/(kg·d)皮下注射,手术组、手术+哮喘组采用切除双侧全部肾上腺复制为肾阳虚证候模型。哮喘组、激素+哮喘组、手术+哮喘组采用卵清白蛋白(OVA)致敏诱发哮喘。观察各组大鼠一般状态、行为学、肺功能及肺泡灌洗液中白细胞分类,同时检测血清下丘脑-垂体-肾上腺(HPA)轴功能指标[包括促肾上腺皮质激素释放激素(CRH)、肾上腺皮质激素释放激素(ACTH)、皮质醇(CORT)、干扰素-γ(INF-γ)、免疫球蛋白E(Ig E)]及肺组织各项炎症因子水平;观察肺组织病理改变。结果激素+哮喘组、手术+哮喘组肺泡灌洗液中白细胞、嗜酸性细胞总数升高,CRH、ACTH、CORT分泌减少;血清总Ig E、OVA特异性Ig E含量升高;肺组织内INF-γ表达分泌减少,白细胞介素4(IL-4)表达分泌增多(P0.05或P0.01);肺组织病理显示支气管出现大量炎症浸润、气道黏膜水肿。激素+哮喘组与手术+哮喘组比较,在肺泡灌洗液炎症细胞检测上无明显差异,后者HPA轴功能指标下降更加明显,前者大鼠肺组织的病理改变更加明显。结论两种肾阳虚证支气管哮喘动物模型均能够反映肾阳虚证支气管哮喘的发病特点,但采用切除双侧全部肾上腺方法的动物模型对HPA轴功能影响更明显。
[Abstract]:Objective to evaluate the modeling method of two kinds of kidney yang deficiency syndrome combined with animal model of bronchial asthma. Methods 54 Wistar rats were divided into the blank group, the asthma group, the hormone group, the hormone + asthma group, the operation group, the operation + asthma group, and 9 rats in each group. The rats in the hormone group and the hormone plus asthma group were subcutaneously injected with Hydrocortisone Injection 15 mg/ (kg. D). The operation group and the operation plus asthma group were replicated with bilateral adrenal glands to form the kidney yang deficiency syndrome model. Asthma was induced by ovalbumin (OVA) sensitization in the asthma group, the hormone + asthma group, and the operation + asthma group. Observe the general state and behavior of rats, pulmonary function and alveolar lavage fluid white blood cell classification, detection of serum and hypothalamic pituitary adrenal (HPA) axis function index including corticotropin releasing hormone (CRH) and corticotropin releasing hormone (ACTH), cortisol (CORT) and interferon gamma (INF-), immunoglobulin E (Ig E)] the level of inflammatory factors in lung tissue and the lung pathological changes were observed. The hormone + asthma group, asthma group operation + bronchoalveolar lavage and elevated white blood cell, eosinophil count in BALF, CRH, ACTH, CORT secretion decreased; elevated total serum Ig E, OVA specific Ig E content; reduce the secretion of INF- gamma expression in lung tissue, interleukin 4 (IL-4) expression and secretion increased (P0.05 or P0.01); lung pathology showed bronchial inflammatory infiltration, airway mucosal edema. There was no significant difference in the detection of inflammatory cells in the bronchoalveolar lavage fluid between the hormone + asthma group and the operation + asthma group. The HPA axis function index of the latter group decreased more obviously, and the pathological change of the lung tissue in the former group was more obvious. Conclusion two animal models of kidney yang deficiency bronchial asthma can reflect the characteristics of bronchial asthma in kidney yang deficiency syndrome. However, animal models with bilateral adrenal gland removal have more significant effects on HPA axis function.
【作者单位】：浙江中医药大学附属湖州中医院;浙江中医药大学动物实验研究中心;
【基金】：国家自然科学基金(81403374,81573677) 浙江省公益性技术应用研究计划项目(2013C37008)
【分类号】：R259;R-332
【正文快照】： 支气管哮喘(简称“哮喘”)是一种由多细胞和细胞组分参与的气道慢性炎症性疾病[1],属于中医学“喘证”“哮证”范畴。中医学认为,哮喘是由于脏腑亏虚、痰瘀伏留、外邪引发所致的本虚标实之证,与肾的关系密切。哮喘患者无论是在缓解期,还是在发作期均存在肾虚,尤其是肾阳虚,从

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