微量MIC法对结核分枝杆菌异质性的应用研究

发布时间：2017-12-27 02:10

本文关键词：微量MIC法对结核分枝杆菌异质性的应用研究　出处：《中华医院感染学杂志》2016年23期 　论文类型：期刊论文

【摘要】：目的评价微量MIC法在结核分枝杆菌异质性研究中的应用价值,为临床提供参考依据。方法选取2014年1月-2015年12月60例结核病患者痰液利用利福平和异烟肼MGIT 960液体培养法和改良罗氏比例法检测药敏结果不一致的分离株标本,微量MIC法检测其对两种药物的敏感性,线性探针杂交法检验其基因型表达情况。结果对同一患者异质性分离株,微量MIC法与改良罗氏比例法药敏结果差异有统计学意义(P0.01),微量MIC法与MGIT 960法差异无统计学意义。结论微量MIC法可用于结核分枝杆菌异质性的筛查,同时联合线性探针杂交法检测可以对结核分枝杆菌的耐药行检测提供早期、快速诊断,并为临床个体化用药提供依据。
[Abstract]:Objective to evaluate the application value of micro MIC in the study of Mycobacterium tuberculosis heterogeneity, and to provide reference for clinical study. Methods from January 2014 -2015 year in December 60 cases of sputum tuberculosis patients using MGIT 960 liquid detection of rifampin and isoniazid susceptibility results inconsistent isolated specimen method and L-J culture method to detect the proportion, drug sensitivity to two kinds of micro MIC method, linear probe hybridization method to test the gene expression of type. Results there was a significant difference in the sensitivity between the micro MIC method and the modified Roche ratio method (P0.01). There was no significant difference between the micro MIC method and the MGIT 960 method. Conclusion the micro MIC method can be used for the screening of Mycobacterium tuberculosis heterogeneity. Combined with linear probe hybridization method, it can provide a quick and early diagnosis for the detection of drug resistance of Mycobacterium tuberculosis, and provide a basis for clinical individualized medication.
【作者单位】：天津市海河医院天津市呼吸疾病研究所;国家中医药管理局中医药防治传染病重点研究室;
【基金】：天津市卫生局基金资助项目(2014KZ035)
【分类号】：R378.911
【正文快照】： 在当今世界,由于贫困、人口剧增,耐多药结核菌的出现给结核病的诊治带来了很大的困难[1-2]。引起结核菌产生耐药的原因很多,其中异质性是其耐药产生过程中的一个细小变化过程,在我们常规的药敏试验中,由于其检测方法的局限性,有时会出现药敏结果不一致的现象,提示我们异质性对

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