胃泌素释放肽及其受体在AEW诱发的小鼠干皮症模型瘙痒中的作用

发布时间：2017-12-28 06:08

本文关键词：胃泌素释放肽及其受体在AEW诱发的小鼠干皮症模型瘙痒中的作用　出处：《中国皮肤性病学杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的建立丙酮/二乙醚溶液和水方法诱发的C57BL/6小鼠干皮症模型(AEW模型),检测胃泌素释放肽(GRP)及受体(GRPR)在颈段背根神经节(DRG)及脊髓的表达,并探讨它们在AEW诱发的干皮症慢性瘙痒发病机制中的作用。方法外用丙酮/二乙醚混合液(1∶1)和水7d建立C57BL/6小鼠的AEW模型,观察小鼠搔抓行为学,皮肤干燥症状,皮肤组织学(HE染色)和肥大细胞浸润(甲苯胺蓝染色)等指标,定量PCR分析GRP在颈部背根神经节(DRG),GRPR在颈部脊髓的mRNA表达水平变化。结果与对照组相比,AEW模型组小鼠的自发性搔抓次数明显多于对照组;AEW模型组小鼠颈背部皮肤表现出明显干燥、脱屑,病理提示表皮增厚、真皮肥大细胞浸润不明显;在颈部DRG的GRP、在颈部脊髓的GRPR mRNA表达水平升高。结论成功建立了干皮症且伴有强烈搔抓行为的C57BL/6小鼠模型,GRP和GRPR在AEW模型瘙痒中有重要作用。
[Abstract]:Objective to establish the acetone / ether solution and method of water induced by two C57BL/6 mice of dry skin disease model (AEW model), the detection of gastrin releasing peptide (GRP) and its receptor (GRPR) in the dorsal root ganglion (DRG) expression and cervical spinal cord, and to explore them in AEW induced chronic dry skin itching disease pathogenesis the role of. Methods topical acetone / two mixture of ether (1: 1) and the establishment of C57BL/6 mouse 7d AEW water model, observe the mouse scratching behavior, dry skin symptoms, skin histology (HE staining) and mast cell infiltration (toluidine blue staining) and other indicators, quantitative analysis of GRP PCR in cervical dorsal root ganglion (DRG) GRPR, expression of mRNA in cervical spinal cord. Results compared with the control group, AEW model group, the spontaneous scratch catch number was more than the control group; AEW model group, the back of the neck skin showed obvious dryness, desquamation, thickening of the epidermis and dermal pathology of mast cell infiltration was not obvious; in the expression of GRP, DRG of cervical spinal cord in the neck of the GRPR mRNA level increased. Conclusion a C57BL/6 mouse model with severe scratch behavior has been established successfully. GRP and GRPR play an important role in AEW model pruritus.
【作者单位】：广州医科大学附属第二医院广东省过敏反应与免疫重点实验室呼吸疾病国家重点实验室变态反应研究室;
【基金】：广东省科技厅公益研究与能力建设专项资金项目(2014A020212343) 广州市过敏反应临床医学研究与转化中心建设项目(穗科创字[2016]171号)
【分类号】：R-332;R758.5
【正文快照】： 表1 GRP,GRPR和GAPDH的引物序列Tab.1 Primers of GRP,GRPR and GAPDHTarget Forward primer(5'-3')Reverse primer(5'-3')GRP TGGGCTGTGGGACACTTAAT GCTTCTAGGAGGTCCAGCAAAGRPR TGATTCAGAGTGCCTACAATCTTC CTTCCGGGATTCGATCTGGAPDH CCCAGCAAGGACACTGAGCAA TTATGGGGGTCTGGGATGG

【相似文献】