上颌窦黑色素瘤原位裸鼠模型的建立及其生物学特征

发布时间：2017-12-28 06:26

本文关键词：上颌窦黑色素瘤原位裸鼠模型的建立及其生物学特征　出处：《临床耳鼻咽喉头颈外科杂志》2017年20期 　论文类型：期刊论文

【摘要】：目的:建立稳定的上颌窦黑色素瘤原位裸鼠模型,为研究鼻腔鼻窦恶性肿瘤的发病机制及治疗手段提供动物模型。方法:将B16(小鼠黑色素瘤细胞)细胞悬液注射到裸鼠右上颌窦,待裸鼠形成原位瘤后,监测肿瘤的生长发展和荷瘤鼠的生存情况,并利用MRI技术观察肿瘤局部浸润情况。结果:在裸鼠上颌窦接种B16细胞,上颌窦出现原位瘤,裸鼠生存率和原位瘤体积大小与接种肿瘤细胞数量有关。MRI显示原位瘤向鼻腔、眼眶、颅底浸润。结论:成功构建了上颌窦黑色素瘤原位裸鼠模型,为进一步研究鼻腔鼻窦恶性肿瘤的生物学特征及新的治疗策略提供了可行的实验工具。
[Abstract]:Objective: to establish a stable orthotopic nude mouse model of the maxillary sinus melanoma, and to provide an animal model for the study of the pathogenesis and treatment of nasal sinus malignant tumor. Methods: B16 (mouse melanoma cell) suspension was injected into the right maxillary sinus of nude mice. After the orthotopic tumor was formed in nude mice, the growth and development of tumor and the survival of tumor bearing mice were monitored. MRI technique was used to observe the local infiltration of tumor. Results: in the maxillary sinus of nude mice, B16 cells were inoculated in nude mice and the maxillary sinus appeared in situ tumor. The survival rate of nude mice and the size of the tumor in situ were related to the number of tumor cells inoculated. MRI showed the invasion of the nasal cavity, the orbit and the skull base of the tumor in situ. Conclusion: a nude mouse model of maxillary sinus melanoma was successfully constructed, which provided a feasible experimental tool for further study of the biological characteristics and new therapeutic strategies of sinonasal malignancies.
【作者单位】：西南医科大学附属医院耳鼻咽喉头颈外科;华西医院耳鼻咽喉头颈外科;成都市第七人民医院耳鼻咽喉头颈外科;华西医院生物治疗国家重点实验室;西南医科大学;
【基金】：四川省科技厅支撑项目(No:2012SZ0024)
【分类号】：R-332;R739.62
【正文快照】： 上颌窦恶性肿瘤约占鼻腔鼻窦恶性肿瘤的80%,是常见的头颈部恶性肿瘤[1]。上颌窦恶性肿瘤最常见的病理类型为鳞状细胞癌,而黑色素瘤是其少见的病理类型。研究表明,头颈部黏膜黑色素瘤占全部黏膜黑色素瘤的50%~55%[2],尤以上颌窦黑色素瘤较常见。目前,上颌窦恶性肿瘤采用手术为

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