热补针法对RA模型家兔关节滑膜LDH、ATPase活性的影响

发布时间：2017-12-28 08:40

本文关键词：热补针法对RA模型家兔关节滑膜LDH、ATPase活性的影响　出处：《中医研究》2016年01期 　论文类型：期刊论文

【摘要】：目的:从机体能量代谢的角度探讨热补针法"取热"的作用机制。方法:将40只青紫蓝家兔随机分为正常对照组、模型对照组、平补平泻组、捻转补法组和热补针法组,每组8只。除正常对照组外,采用卵蛋白诱导法和低温冷冻法对其他4组进行RA寒证模型复制。造模成功后,除正常对照组、模型对照组外,其余各组分别施以平补平泻、捻转补法、热补针法针刺,留针30 min/次,1 d 1次,共7 d。治疗结束后分离关节滑膜,冰冻切片,借助组织化学染色技术观察膝关节滑膜组织乳酸脱氢酶(LDH)、三磷酸腺苷酶(ATPase)活性。结果:模型对照组LDH和ATPase积分光度、阳性总面积、面积百分比高于正常对照组,差别有统计学意义(P0.05);针刺组LDH活性变化均低于模型对照组(P0.05),而ATPase活性变化均高于模型对照组(P0.05);热补针法组LDH活性变化均低于其他两针刺组(P0.05),但ATPase活性变化明显高于其他两针刺组(P0.05)。结论:热补针法能够降低RA家兔关节滑膜LDH活性,增强ATPase活性,从而增强有氧代谢,使机体局部产生更多的能量可能是热补针法"取热"的机制之一。
[Abstract]:Objective: To investigate the mechanism of heat reinforcing needling "heat" from the angle of energy metabolism. Methods: 40 Chinchilla rabbits were randomly divided into normal control group, model control group, reinforcing reducing group, acupuncture group and acupuncture group have 8 rats in each group. In addition to the normal control group, the RA cold syndrome model was replicated in the other 4 groups by ovalbumin induction and cryopreservation. After the success of the model, in addition to the normal control group, model control group, the other groups were treated with reinforcing reducing, twisting method, heat reinforcing needling acupuncture needle for 30 min/, 1 D 1, 7 d. After the end of treatment from synovial, frozen section technique to observe the knee joint synovial tissues by lactate dehydrogenase histochemical staining (LDH) and adenosine triphosphatase (ATPase) activity. Results: the model control group LDH and ATPase integral luminosity, the total positive area, area percentage is higher than the normal control group, the difference was statistically significant (P0.05); changes in the activity of LDH acupuncture were lower than that of model group (P0.05), and the changes of ATPase activity were higher than those in the model control group (P0.05); changes of LDH activity were hot acupuncture group lower than the other two acupuncture group (P0.05), but the change of ATPase activity was significantly higher than the other two acupuncture group (P0.05). Conclusion: acupuncture can reduce the heat RA of rabbit synovial LDH activity, increased ATPase activity, thereby enhancing aerobic metabolism, make the body produce local mechanism of more energy may be "hot" heat reinforcing needling.
【作者单位】：甘肃中医药大学;兰州大学第二医院;
【基金】：2011年度甘肃省教育厅第二批科研项目(1106B-08) 2010年度甘肃中医学院中青年科研基金项目(gszyxy2010-4)
【分类号】：R245;R-332
【正文快照】： 热补针法是简化烧山火和进火补法所形成的一种针刺手法,具有温阳散寒的作用,能使患者产生热感[1]。研究[2-4]表明:类风湿关节炎(rheumatoid ar-thritis,RA)患者存在能量代谢不足的现象,即轻中度贫血、四肢关节冰冷僵硬等。前期的研究[5-8]证实了热补针法对RA模型家兔具有镇痛

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