两种健脾产品对脾虚证复合抗生素大鼠模型肠道菌群的影响

发布时间：2017-12-28 08:27

本文关键词：两种健脾产品对脾虚证复合抗生素大鼠模型肠道菌群的影响　出处：《中成药》2017年10期 　论文类型：期刊论文

【摘要】：目的考察两种健脾产品常欣卫和益生菌中草药复合制剂对肠道菌群的调节作用。方法复制脾虚复合抗生素大鼠模型,采用16 s r DNA测序方法检测大鼠粪便中菌群的变化,通过偏最小二乘法及聚类分析等方法考察常欣卫和益生菌中草药复合制剂对大鼠肠道菌群的影响。结果相对于正常组,脾虚复合抗生素模型大鼠肠道菌群结构发生显著改变,其中厚壁菌门比例明显增高,拟杆菌门、放线菌门比例明显降低;厌氧杆菌属、罗氏菌属、梭菌属ⅩⅧ、埃希氏菌属、假丁酸弧菌属、氮单胞菌属、不动杆菌属、假单胞菌属、黄杆菌属、沙雷氏菌属等比例均显著增高,拟杆菌属、普氏菌属、乳杆菌属、消化球菌属、坦纳菌属、醋弧菌属等比例均显著降低。健脾产品常欣卫对模型大鼠氮单胞菌属、不动杆菌属、假单胞菌属、黄杆菌属、沙雷氏菌属比例有显著的下调作用;对拟杆菌属、普氏菌属、乳杆菌属等有显著上调作用;益生菌中草药复合制剂对模型大鼠厌氧杆菌属、罗氏菌属、梭菌属ⅩⅧ、假丁酸弧菌属、氮单胞菌属、不动杆菌属、假单胞菌属、黄杆菌属、沙雷氏菌属等有显著下调作用;对乳杆菌属、双歧杆菌属等有显著上调作用。结论两种健脾产品均能够一定程度的抑制脾虚复合抗生素模型大鼠肠道潜在致病菌属,提高有益菌属的比例,进而调节菌群的紊乱状态。
[Abstract]:Objective to investigate the regulatory effect of compound herbal medicine for invigorating spleen and two products Xinwei probiotics on intestinal microflora. Methods the rat model of spleen deficiency combined with antibiotics 16 S R replication, DNA sequencing by changing the method of bacteria detection in feces of rats, by partial least squares method and cluster analysis method to study the effect of compound herbal medicine often Xinwei and probiotics on the intestinal microflora of rats. Results compared with normal group, spleen deficiency combined with antibiotics model rats showed intestinal flora structure changed significantly, including Firmicutes increased proportions of Bacteroidetes, actinobacteria decreased significantly; anaerobic bacteria of the genus Clostridium sp., Roche, XVIII, Escherichia coli, Vibrio, single nitrogen false butyric acid bacteria, Acinetobacter, Pseudomonas, Flavobacterium, Serratia ratio were significantly increased, Bacteroides and Prevotella, Lactobacillus, Staphylococcus, digestive bacteria, Vibrio Tanner vinegar ratio decreased significantly. The spleen products on a rat model of Xinwei azomonas, Acinetobacter, Pseudomonas, Flavobacterium, Serratia ratio have significant down-regulation; of Bacteroides Prevotella, and milk increased effect of Bacillus probiotics on compound herbal medicine; model the rats in anaerobic bacillus, Rothia, Clostridium XVIII, false butyrivibrio, azomonas, Acinetobacter, Pseudomonas, Flavobacterium, Serratia have significant downregulation of; Lactobacillus and Bifidobacterium are significantly upregulated. Conclusion two kinds of invigorating spleen products can inhibit the intestinal potential pathogenic bacteria of the spleen deficiency and compound antibiotic rats to a certain extent, improve the proportion of beneficial bacteria, and then regulate the state of the bacteria.
【作者单位】：无限极(中国)有限公司;广州中医药大学脾胃研究所;沈阳药科大学中药学院;
【分类号】：R285.5;R-332
【正文快照】： 脾虚证临床表现主要为舌质淡或胖嫩、有齿印,舌苔白润、食欲减退、肢体怠倦或神疲懒言、食后饱胀或腹胀、大便不正常(稀溏,先硬后溏,时溏时硬)、脉象缓弱或细弱、沉弱,或虚大。其发病原因与生活饮食习惯、压力等因素有关。同时我国对于抗生素的临床使用率较高的情况也对肠道菌

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