疏风解毒胶囊治疗EB病毒感染致裸鼠肿瘤模型的药效学探讨

发布时间：2017-12-28 18:40

本文关键词：疏风解毒胶囊治疗EB病毒感染致裸鼠肿瘤模型的药效学探讨　出处：《中国实验方剂学杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:通过观察疏风解毒胶囊对EB病毒转染的人鼻咽癌细胞株CNE-2Z移植感染Balb/C裸小鼠致肿瘤模型的影响,评价疏风解毒胶囊体内抗EB病毒作用。方法:在Balb/C裸鼠右腋皮下接种鼻咽癌CNE-2Z细胞悬液,接种1周后,将移植瘤模型成功的裸鼠随机分为6组,分别为模型组、阿昔洛韦组、连花清瘟胶囊组、疏风解毒胶囊高、中、低剂量组(2.2,1.1,0.55 g·kg~(-1)·d~(-1)),每组7只,分组当天开始给药,每日1次,连续给药3周。给药当天测定裸鼠的体重,肿瘤体积,隔日测定1次,3周后称体重处死裸小鼠,取出肿瘤组织、脾脏和胸腺称质量,计算每组肿瘤体积、相对肿瘤体积、相对肿瘤增殖率、脾脏指数、胸腺指数。结果:与模型组比较,疏风解毒胶囊高、中、低剂量组均可在一定程度上降低Balb/C裸小鼠脾脏指数,升高其胸腺指数;疏风解毒胶囊高剂量组可显著降低给药8~20 d后裸鼠肿瘤体积(P0.05,P0.01);中剂量组可显著降低给药8~14,18 d后肿瘤体积(P0.05,P0.01),低剂量组可显著降低给药8,12 d后肿瘤体积(P0.05)。在相对肿瘤增值率方面疏风解毒胶囊表现出一定的抑制肿瘤增长的趋势。结论:疏风解毒胶囊对EB病毒转染的CNE-2Z细胞移植裸小鼠肿瘤模型具有抑制作用。
[Abstract]:Objective: To observe the effect of Shu Feng Jiedu Capsule on the tumor model induced by EB virus transfected human nasopharyngeal carcinoma cell line CNE-2Z infected with Balb/C nude mice, and to evaluate the anti EB effect of Shu Feng Jiedu Capsule in vivo. Methods: Balb/C nude mice subcutaneously inoculated with CNE-2Z nasopharyngeal carcinoma cell suspension transplantation 1 weeks after inoculation, the mice were randomly divided into 6 groups of successful models, including model group, acyclovir group, Lianhuaqingwen capsule group, Shufengjiedu capsule high, medium and low dose group (2.2,1.1,0.55 G - kg~ (-1) - d~ (-1)), 7 rats in each group. The group started the day administered 1 times daily for 3 weeks. The body weight and tumor volume of nude mice were measured on the same day. 1 days later, 3 weeks later, the nude mice were weighed. The tumor tissues, spleen and thymus were weighed. The tumor volume, relative tumor volume, relative tumor proliferation rate, spleen index and thymus index were calculated. Results: compared with the model group, Shufengjiedu capsule high, medium and low dose group can be reduced to some extent Balb/C nude mice spleen index, increase the thymus index; Shufengjiedu capsule high dose group can significantly reduce the volume of administration of 8~20 d after tumor (P0.05, P0.01); middle dose group significantly d after the Administration of 8~14,18 reduced tumor volume (P0.05, P0.01), low dose group can significantly reduce tumor volume after administration of 8,12 D (P0.05). In terms of relative value of tumor value, the capsule of detoxification and detoxification can inhibit the growth of tumor. Conclusion: the capsule of Shufeng Jiedu can inhibit the tumor model of nude mice transplanted with EB virus transfected by CNE-2Z cells.
【作者单位】：中国中医科学院中药研究所;
【分类号】：R285.5;R-332
【正文快照】： [网络出版地址]http://www.cnki.net/kcms/detail/11.3495.R.20170210.0858.006.html[网络出版时间]2017-02-10 8:58EB病毒(epstein-barr virus,EBV),又称人类疱疹病毒4型,是一种线性双链排列的DNA病毒,主要感染人类口咽部的上皮细胞和B淋巴细胞,其增殖后入血致病毒性血症,从

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