机械和感染双重法建立中华田园犬宫腔粘连模型

发布时间：2017-12-28 18:45

本文关键词：机械和感染双重法建立中华田园犬宫腔粘连模型　出处：《重庆医科大学学报》2017年04期 　论文类型：期刊论文

【摘要】：目的:通过机械和感染双重法构建一个犬的宫腔粘连动物模型,并检测血管内皮因子(vascular endothelial growth factor,VEGF)、转化生长因子(transforming growth factor-β1,TGF-β1)的表达,评估其与宫腔粘连形成的关系,为下一步研究提供实验平台。方法:选取6只性发育成熟的健康雌性中华田园犬,所有建模前的犬的一小段右侧宫角组织作为对照组,建模后该部位子宫角组织作为处理组。利用机械搔刮和感染双重法损伤右侧宫角内膜,于建模前后各收集一小段宫角标本,行HE、Masson染色及SP法免疫组化,整理并统计分析。结果:处理组的内膜厚度[(2.325±0.399)cm]薄于对照组[(3.025±0.466)cm],差异具有统计学意义(P=0.050);处理组的内膜腺体数目[(4.388±1.774)个]明显比对照组[(16.518±4.421)个]减少,差异具有统计学意义(P=0.001);处理组的子宫内膜纤维化面积比例(0.775±0.040)明显高于对照组(0.329±0.062),差异有统计学意义(P=0.000);处理组的子宫内膜中VEGF(5.967±0.390)、TGF-β1(6.200±0.306)的表达量高于对照组(1.700±0.823)、(1.100±0.361),差异均具有统计学意义(P=0.000,P=0.001)。结论:采用机械和感染双重法可以成功构建中华田园犬宫腔粘连的动物模型,且VEGF、TGF-β1的表达量升高可能与宫腔粘连的发生有关。
[Abstract]:Objective: intrauterine adhesions animal model by mechanical method to build a double infection and canine, and detection of vascular endothelial growth factor (vascular endothelial growth factor VEGF (transforming), transforming growth factor beta 1 beta growth factor-, TGF- 1) expression, and evaluate the relationship between the formation of intrauterine adhesions, provides an experimental platform for the next step of the research. Methods: a total of 6 sexually mature healthy female Chinese pastoral dogs were selected. A small segment of the right cornual tissue of all the dogs before modeling was taken as the control group. After modeling, the uterine horn tissue in this part was taken as the treatment group. The right cornu's intima was damaged by mechanical scraping and infection. A small section of Cornu's specimen was collected before and after modeling. HE, Masson staining and SP immunohistochemical staining were used for statistical analysis. Results: the treatment group endometrial thickness [(2.325 + 0.399) cm] thinner than that of the control group [(3.025 + 0.466) cm], the difference was statistically significant (P=0.050); the treatment group the number of endometrial glands (4.388 + 1.774) [a] was significantly better than the control group [(16.518 + 4.421)] a decreased statistically significant differences (P=0.001); treatment of endometrial fibrosis area ratio group (0.775 + 0.040) was significantly higher than the control group (0.329 + 0.062), the difference was statistically significant (P=0.000); VEGF group of endometrium (5.967 + 0.390), TGF- beta 1 (6.200 + 0.306) expression was higher than that of the control group (1.700 + 0.823), (1.100 + 0.361), the differences were statistically significant (P=0.000, P=0.001). Conclusion: the animal models of intrauterine adhesions in Chinese pastoral dogs can be successfully constructed by dual mechanical and infection methods, and the increased expression of VEGF and TGF- beta 1 may be related to the occurrence of intrauterine adhesions.
【作者单位】：重庆医科大学附属大学城医院妇产科;
【基金】：重庆市卫生计生委医学科研项目(编号:20141014) 重庆市医疗特色专科建设项目(编号:2013-46)
【分类号】：R711.74;R-332
【正文快照】： 宫腔粘连(intrauterine adhesions,IUA)也称阿谢曼综合征(Asherman syndrome,AS),指各种因素使子宫内膜基底层损伤后,在创伤愈合的过程中发生纤维化病变,从而引起宫腔部分或全部粘连的现象[1]。通常发生在刮宫术或任何子宫手术后,包括子宫肌瘤切除术、腹腔镜成形术等,也可见于

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