左归降糖解郁方对糖尿病并发抑郁症模型大鼠海马NR不同亚型表达的影响

发布时间：2017-12-30 23:10

本文关键词：左归降糖解郁方对糖尿病并发抑郁症模型大鼠海马NR不同亚型表达的影响　出处：《时珍国医国药》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨左归降糖解郁方对糖尿病并发抑郁症模型大鼠海马NR不同亚型NR2A、NR2B表达的影响。方法建立糖尿病并发抑郁症大鼠模型,并随机分为5组:模型组,阳性药(二甲双胍0.18g/kg+盐酸氟西汀1.8mg/kg)组,左归降糖解郁方高、中、低剂量(32.82,16.41,8.20g/kg)组,以正常大鼠为空白对照组,每组10只,灌胃给药28天。给药后,采用Morris水迷宫实验评价大鼠行为学变化,Western-blotting和RT-PCR法定量检测各组大鼠海马NR2A、NR2B相关基因及蛋白的表达情况。结果与空白对照组比较,模型组大鼠(P0.01)逃避潜伏期延长,空间探索时间减少,海马NR2A、NR2B表达明显升高(P0.01),差异有显著统计学意义;与模型组比较,阳性药组和左归降糖解郁方高剂量组大鼠逃避潜伏期明显缩短,空间探索时间增加(P0.01),海马NR2A、NR2B的表达减少(P0.05),差异有统计学意义。结论左归降糖解郁方可通过调控DD大鼠海马NR2A、NR2B的表达,明显改善DD大鼠的抑郁样行为。
[Abstract]:Objective to investigate the effect of Zuo Gui Jiangtang Jie Yu recipe (ZJD) on the expression of NR2A1NR2B in hippocampus of diabetic rats with depression. Methods the rat model of diabetes mellitus complicated with depression was established. They were randomly divided into 5 groups: model group, positive group (metformin 0.18g / kg fluoxetine 1.8mg / kg), left Guijiangtang Jieyu prescription with high, middle and low doses of 32.82. The rats in the 16.41 ~ 8.20 g / kg group were treated with Morris water labyrinth test for 28 days, 10 rats in each group were treated by gavage for 28 days. The expression of NR2A- NR2B related genes and proteins in hippocampus of rats in each group was assayed by Western-blotting and RT-PCR. The results were compared with those of the control group. In the model group, the escape latency was prolonged, the spatial exploration time was decreased, and the expression of NR2A- NR2B in hippocampus was significantly increased (P 0.01). Compared with the model group, the escape latency of the rats in the positive drug group and the high dose group of Zuoguijiangjiayu prescription was significantly shortened, the spatial exploration time was increased (P 0.01), and the hippocampal NR2A was increased. Conclusion Zuogui Jiangtang Jieyu decoction can regulate the expression of NR2A- NR2B in hippocampus of DD rats. The depressive behavior of DD rats was significantly improved.
【作者单位】：湖南中医药大学;湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地;湖南中医药大学第一附属医院;
【基金】：国家自然科学基金(No.81373578;No.81403379;No.81573965)
【分类号】：R285.5;R-332
【正文快照】： 全世界约有3.47亿糖尿病患者,而糖尿病并发抑郁症(Dia-betes mellitus with depression,DD)的发生率为8.5%~71.4%[1],其患者自杀的危险率高达10%,远高于普通人群的10~30/10万人[2]。目前,针对DD的研究多集中于临床试验,极少数涉及其分子机制的研究,极大的限制了治疗DD特效药的

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