斑马鱼模型在糖尿病研究中的应用

发布时间：2017-12-30 23:26

本文关键词：斑马鱼模型在糖尿病研究中的应用　出处：《中国比较医学杂志》2017年08期 　论文类型：期刊论文

【摘要】：作为一种模式生物,斑马鱼具有其他动物模型所不具备的诸多优势,非常适用于进行人类疾病建模及机制研究。斑马鱼胰腺经历两次发育分化过程,其间伴有多种信号途径的活化参与。成鱼的胰腺结构与其他动物胰腺相似,能分泌包括胰岛素在内的多种激素。斑马鱼胰腺及对胰岛素敏感的肝脏、肌肉等这些与代谢调控相关的主要器官及外周组织,在进化上具有保守性,糖代谢调控机制也与其他哺乳动物相似,这使得斑马鱼非常适用于糖代谢调控研究。除利用STZ诱导胰岛β细胞凋亡造成斑马鱼糖尿病模型外,高糖溶液交替暴露及外源性干预代谢相关信号途径也可以造成斑马鱼血糖升高。成鱼血糖水平、幼鱼组织糖含量、视网膜及视网膜血管形态变化是目前斑马鱼糖尿病模型研究中的常用检测指标。
[Abstract]:As a model organism, zebrafish have many advantages that other animal models do not have, which is very suitable for human disease modeling and mechanism research. Zebrafish pancreas undergo two developmental and differentiation processes. The pancreatic structure of adult fish is similar to that of other animal pancreas, and it can secrete many hormones, including insulin, zebrafish pancreas and insulin-sensitive liver. These major organs and peripheral tissues related to metabolic regulation, such as muscle, are evolutionarily conserved, and the regulation mechanism of glucose metabolism is similar to that of other mammals. This makes zebrafish very suitable for the regulation of glucose metabolism, except for the diabetic model of zebrafish induced by STZ. Alternate exposure to high glucose solutions and exogenous metabolic related signaling pathways also resulted in increased blood glucose levels in adult fish and sugar content in young fish tissues. Retinal and retinal vascular morphological changes are commonly used in the study of zebrafish diabetic model.
【作者单位】：山东省科学院生物研究所山东省生物检测技术工程实验室山东省科学院药物筛选技术重点实验室;
【基金】：国家自然科学基金资助项目(31400979) 山东省自主创新重大专项(2014ZZCX0215)
【分类号】：R-332;R587.1
【正文快照】： 糖尿病是一组由多病因引起的、以慢性高血糖为特征的终身性代谢性疾病。糖尿病患者体内长期存在的高血糖状态,会广泛导致机体各组织,尤其是眼、肾、心脏、血管及神经等的慢性损害和功能障碍,严重危害人体健康。糖尿病是全球流行性疾病,患病率呈逐年增高趋势,并成为致死和致残

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