活性氧及酸性鞘磷脂酶在工频磁场诱导的FL细胞膜受体聚簇中的作用

发布时间：2017-12-31 01:42

本文关键词：活性氧及酸性鞘磷脂酶在工频磁场诱导的FL细胞膜受体聚簇中的作用　出处：《浙江大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的研究工频磁场诱导细胞膜受体聚簇的机制。方法用0.4mT工频磁场辐照处理人羊膜(FL)细胞,采用鞘磷脂酶活性试剂盒检测该磁场辐照对胞内A-SMase活性的影响,利用活性氧检测技术分析工频磁场辐照对FL细胞内ROS水平的影响,利用激光共聚焦显微镜免疫荧光技术分析活性氧在工频磁场诱导的细胞膜受体聚簇中的作用,利用酶标定量测定仪的酶标检测技术系统的分析细胞内A-SMase、ROS在该磁场辐照诱导的膜表面EGF受体聚簇中的相互关系,采用方差分析和SNK检验统计处理数据。结果工频磁场辐照15min组和30min组FL细胞内的A-SMase活性升高,且差异均有统计学意义(P0.05)；工频磁场辐照15min引起FL细胞内活性氧水平增加,差异有统计学意义(P0.05),而经PDTC预处理后,辐照组细胞内活性氧水平比未经PDTC处理组低,且差异有统计学意义(P0.05)。工频磁场辐照组发生受体聚簇的细胞百分比显著增加,差异具有统计学意义(P0.05)；PDTC预处理可显著抑制工频磁场辐照对细胞内A-SMase活性的诱导作用,差异也具有统计学意义(P0.05)。结论工频磁场诱导的FL细胞膜表面EGF受体聚簇与ROS有密切关系；工频磁场通过增加细胞内ROS的水平提高A-SMase活性。
[Abstract]:Objective to study the mechanism of magnetic field induced cell membrane receptor clustering. 0.4mT power frequency magnetic field irradiation method using human amniotic membrane (FL) cells by sphingomyelinase kit for detecting the magnetic field irradiation effect on the activity of A-SMase in cells, effects of power frequency magnetic field irradiation on the level of ROS in FL cells analyzed by active oxygen detection technology. Confocal microscopy immunofluorescence analysis of cell membrane active oxygen in the power frequency magnetic field induced receptor clustering in the role of using the laser measuring instrument using enzyme quantitative ELISA system detection technology analysis of intracellular A-SMase, ROS in the magnetic field irradiation induced membrane EGF receptors on the surface of poly relationship in the cluster, by analysis of variance SNK test and statistical data processing. The results of power frequency magnetic field irradiation of 15min group and 30min group in FL cells increased the activity of A-SMase, and the differences were statistically significant (P0.05); power frequency magnetic field irradiation 15 Min induced ROS level in FL cells increased, the difference was statistically significant (P0.05), and treated with PDTC, the level of reactive oxygen in cells without irradiation group than PDTC treatment group, and the difference was statistically significant (P0.05). The percentage of power frequency magnetic field irradiation group occurred receptor clustering increased significantly, with statistically significant difference (P0.05); PDTC pretreatment could significantly inhibit the irradiation induced power frequency magnetic field effect on A-SMase activity in cells, the difference was statistically significant (P0.05). Conclusion the power frequency magnetic field induced surface EGF FL cell membrane receptor clustering have a close relationship with ROS; the power frequency magnetic field by increasing the intracellular levels of ROS increased by A-SMase activity.

【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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