食用白酒对大鼠心脏ACE、ACE2表达的影响

发布时间：2017-12-31 01:45

本文关键词：食用白酒对大鼠心脏ACE、ACE2表达的影响　出处：《遵义医学院》2012年硕士论文　论文类型：学位论文

【摘要】：目的：在一定时间内连续给大鼠灌胃不同剂量的食用白酒,观察心脏结构、功能及检测心肌组织中ACE、ACE2及AngⅡ的变化。方法： SD大鼠124只按4wk、8wk、12wk三个时间点随机分成白酒组、酒精组、生理盐水组,每组分别又分为低、中和高剂量组,每组6只,共27组。按低剂量(0.25ml/100g/d)、中剂量(0.5ml/100g/d)、高剂量(1ml/100g/d),每日量分两次(每次按半量)给予大鼠灌胃,两次间隔时间超过9h以上。每周称体重一次,根据体重调整一周的灌胃量。分别在上述三个时间点测定左心室内压变化幅度(LVP),计算心脏／体重指数(HW/BW), Masson染色观察心肌纤维化,ELISA检测心肌组织AAngⅡ、ACE、ACE2含量,RT-PCR检测心肌细胞ACEmRNA和ACE2mRNA的表达。结果一：①4wk末白酒组的各剂量组左心室内压变化幅度、HW/BW与酒精组、生理盐水组比较无显著性差异(P0.05)。②4wk末白酒组、酒精组的中、高剂量组的心肌细胞胞浆轻度浑浊、变性,纤维组织轻度增生,且以高剂量变化明显。③4wk末白酒组、酒精组的各剂量组的心肌组织AngⅡ、ACE、ACE2含量与生理盐水组比较,无显著性差异(P0.05),但出现随剂量增加而升高的趋势。④4wk末白酒组、酒精组的各剂量组心肌组织ACEmRNA、ACE2mRNA的表达与生理盐水组比较,无明显变化(P0.05)。结果二：①8wk末白酒组的各剂量组LVP、HW/BW与酒精组、生理盐水组比较无显著性差异(P0.05)。②在8wk末,随着时间延长,从低剂量组到高剂量组的心肌细胞胞浆逐渐发生变性、浑浊,纤维组织逐渐增生明显,各剂量组中以酒精组变化明显。③8wk末白酒的中、高剂量组和酒精的中、高剂量组心肌组织AngⅡ、ACE含量分别高于生理盐水对照组、低剂量组(P0.05,P0.01),且酒精的高剂量组心肌组织ACE含量高于中剂量组(P0.05)。④8wk末白酒和酒精的低、中、高剂量组心肌组织ACE2含量均高于生理盐水对照组(P0.05,P0.01,P0.01),白酒和酒精的中剂量组、高剂量组和心肌组织ACE2含量分别高于各自的低剂量组(P0.05,P0.01),且高剂量组心肌组织ACE2还含量高于中剂量组(P0.05)。⑤8wk末白酒和酒精中、高剂量组的心肌组ACE2mRNA、表达分别高于生理盐水对照组和低剂量组(p0.05,p0.01)。⑥8wk末白酒和酒精中、高剂量组的心肌组织ACEmRNA均高于生理盐水对照组(p0.05,p0.01)。白酒、酒精高剂量组的心肌组织ACEmRNA表达分别高于各自的低剂量组、中剂量组(p0.01,p0.05)。结果三：①12wk末白酒组、酒精组的各剂量组的LVP,HW/BW均比生理盐水组升高(P0.05)。②在12wk末中,随着时间延长,从低剂量组到高剂量组的心肌细胞胞浆浑浊、变性逐步加重,纤维组织逐步明显增生,高剂量组还出现脂肪组织增生,各剂量组中以酒精组变化明显。③12wk末白酒组和酒精组的低、中、高剂量组心肌组织AngⅡ、ACE含量均高于生理盐水对照组(P0.05,P0.01,P0.01),且中、高剂量组的AngⅡ、ACE含量均维持在高水平,高于低剂量组(P0.01),而白酒高剂量组的心肌组织AngⅡ含量高于中剂量组(P0.01)。④12wk末时白酒组和酒精组的低剂量组心肌组织ACE2含量明显高于生理盐水对照组(P0.01),而中、高剂量组的ACE2含量均低于生理盐水对照组(P0.01,P0.01)。⑤12wk末白酒和酒精低剂量组的心肌组ACE2mRNA、ACE2表达均高于生理盐水对照组(p0.01)。白酒和酒精中、高组的的心肌组织ACE2mRNA表达均低于生理盐水对照组(p0.05)。⑥12wk末白酒和酒精低、中、高剂量组的心肌组织ACEmRNA表达分别高于生理盐水对照组(p0.01)白酒和酒精的中、高剂量组的心肌组织ACEmRNA表达分别高于各自的低剂量组(p0.05,p0.01)。白酒和酒精高剂量组的ACEmRNA表达分别高于各自的中剂量组(p0.05,p0.05) 结论：1.短期内少量饮酒对心肌功能无明显损伤,AngⅡ、ACE、ACE2含量无明显差异。2.长期饮酒可导致心肌纤维化,其变化程度与饮酒量以及时间长短有关。3.长期饮酒可破坏心肌内的ACE和ACE2平衡,使ACE和AngⅡ的升高,ACE2降低,导致心肌纤维化。4.在相同时间、相同剂量下,单纯酒精对心脏的损伤重于食用白酒。
[Abstract]:Objective: in a certain period of time, the rats were continuously given different doses of white wine to observe the structure and function of the heart, and to detect the changes of ACE, ACE2 and Ang II in the myocardium.
Methods: 124 SD rats, according to 4wk, 8wk, 12wk three time points were randomly divided into liquor group, alcohol group, normal saline group, each group is divided into low, medium and high dose group, 6 rats in each group, a total of 27 groups. According to the low dose (0.25ml/100g/d), middle dose, high dose (0.5ml/100g/d) (1ml/100g/d), daily amount of two times (by each half) rats were given orally, two times more than 9h. The interval time weighed once a week, according to the weight adjusted the amount of gastric lavage for a week. Left ventricular pressure was determined respectively in the range of three time points (LVP), calculated the heart / body weight index (HW/BW), myocardial fibrosis was observed by Masson staining, detection of myocardial ELISA AAng II, ACE, ACE2 content, RT-PCR to detect the expression of myocardial cells of ACEmRNA and ACE2mRNA.
Results: the amplitude of 4wk at the end of a liquor group each dose group of left ventricular pressure, HW/BW and alcohol group, there was no significant difference between the normal saline group (P0.05). At the end of the 4wk liquor group, alcohol group, high dose group myocardial cells slightly turbid, degeneration, mild proliferation of fibrous tissue, and the dosage change obviously. The 4wk end liquor group, alcohol group each dose group of myocardial tissue of Ang, ACE, ACE2 content compared with the saline group, no significant difference (P0.05), but the increased tendency with the dose. The 4wk end liquor group, alcohol group the myocardial tissue in each dose group of ACEmRNA, the expression of ACE2mRNA compared with the normal saline group, no significant change (P0.05).
Results: at the end of two 8wk liquor group each dose group of LVP, HW/BW and alcohol group, there was no significant difference between the normal saline group (P0.05). At the end of 8wk, with the prolongation of time, from low dose to myocardial cells in high dose group gradually degenerated, muddy, fibrous tissue hyperplasia gradually obviously, each dose group in the alcohol group changed significantly. The 8wk at the end of the liquor, the high dose group and alcohol in the high dose group of myocardial tissue of Ang II, ACE concentrations were higher than those in the saline control group, low dose group (P0.05, P0.01), myocardial tissue of high dose group was higher than that of ACE and the content of alcohol the middle dose group (P0.05). The 8wk at the end of liquor and alcohol, low content of ACE2 in myocardial tissue of high dose group were higher than those in the saline control group (P0.05, P0.01, P0.01), middle dose group of liquor and alcohol, the content of ACE2 in myocardial tissue of high dose group and low dose group were higher than that of the respective (P0.05 P0.01), And the myocardial tissue of high dose group was higher than that in ACE2 medium dose group (P0.05). At the end of the 8wk liquor and alcohol group, myocardial ACE2mRNA high dose group, the expression were higher than that in the saline control group and low dose group (P0.05, P0.01). The 8wk at the end of liquor and alcohol, the high dose group of myocardial tissue ACEmRNA was higher than that of the saline control group (P0.05, P0.01). The expression of alcoholic liquor, the high dose group of ACEmRNA in the myocardial tissue were higher than the low dose group the middle dose group (P0.01, P0.05).
Results: at the end of three 12wk liquor group, alcohol group each dose group of LVP, HW/BW were higher than that of normal saline group (P0.05). At the end of 12wk, with the prolongation of time, from low dose to myocardial cells turbidity high dose group, degeneration increased gradually, gradually obvious hyperplasia of fibrous tissue also, the high dose group of fat tissue, each dose group in the alcohol group changed significantly. The 12wk at the end of liquor group and alcohol group, low and high dose group myocardial Ang II, ACE content were higher than the normal saline control group (P0.05, P0.01, P0.01), and in high dose group Ang II, ACE levels were maintained at a high level, higher than the low dose group (P0.01), and higher myocardial Ang content in liquor of high dose group middle dose group II (P0.01). The 12wk at the end of the myocardial tissue in low dose group ACE2 content of liquor group and alcohol group was significantly higher than the saline control group (P0.01) however, in high dose The content of ACE2 group were lower than the normal saline control group (P0.01, P0.01). The 12wk at the end of liquor and alcohol in low dose group myocardial ACE2mRNA group, the expression of ACE2 was higher than that of the saline control group (P0.01). The liquor and alcohol, high group of myocardial tissue ACE2mRNA expression were lower than the normal saline control group (P0.05 12wk). At the end of liquor and alcohol, low and high dose group of myocardial ACEmRNA expression were higher than those in the saline control group (P0.01) of liquor and alcohol in the high dose group of myocardial ACEmRNA expression were higher than the low dose group respectively (P0.05, P0.01). The liquor and alcohol in high dose group ACEmRNA the expression was higher than that of middle dose group respectively (P0.05, P0.05)
Conclusion: 1. in the short term, a small amount of alcohol had no obvious damage on myocardial function of Ang, ACE, ACE2 and.2. had no significant difference in the long-term drinking can lead to myocardial fibrosis, the degree of change and consumption as well as the related.3. long term alcohol consumption can damage the balance of cardiac ACE and ACE2, increased ACE, ACE2 and Ang II reduced, leading to myocardial fibrosis.4. at the same time, under the same dosage, simple injury to the heart in edible alcohol liquor.

【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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