酸性成纤维细胞生长因子对顺铂诱导的血管内皮细胞损伤的保护作用研究

发布时间：2017-12-31 06:34

本文关键词：酸性成纤维细胞生长因子对顺铂诱导的血管内皮细胞损伤的保护作用研究　出处：《广西医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的:探讨酸性成纤维细胞生长因子(aFGF)对顺铂(DDP)引起的体外培养的血管内皮细胞损伤的保护作用。方法:通过组织块法获取大鼠血管内皮细胞,爬片培养经Ⅷ因子相关抗原免疫细胞化学染色鉴定,纯化后传代接种于96孔培养板,将处于对数生长期的细胞分为三组:空白对照组(Control组)、DDP组、aFGF+DDP组。主要程序如下:(1)培养24 h后加入一系列浓度的DDP,再培养24 h后用MTT法检测细胞存活率;(2)培养24h后在系列DDP浓度的基础上加入一定浓度的aFGF,继续培养24h后用MTT法检测细胞存活率;(3)从形态学上观察aFGF对DDP诱导的血管内皮细胞损伤的保护作用;通过检测Control组、DDP组、aFGF+DDP组相应的生化指标(MDA、NO)和酶学指标(SOD、GSH-Px)的变化,观察aFGF对DDP诱导的血管内皮细胞损伤的保护作用。结果:(1)aFGF能使DDP对血管内皮细胞的半数抑制浓度(IC50)升高。(2)加入2.59mg/L DDP作用24h后可见细胞由原有的单层铺路石样皱缩、变圆,形态异常,立体感较差,细胞间隙变宽,细胞彼此之间的连接减少,细胞边界模糊甚至消失,有些细胞脱落悬浮于培养基中;而10μg/LaFGF+DDP培养24 h,细胞形态有所改善,细胞间隙缩小,细胞间的连接增加,细胞边界较清楚,少数细胞恢复为短梭形。(3)DDP组与Control组比较,MDA含量升高而SOD、NO和GSH-Px的活性降低,差异均有统计学意义(P 0.01);aFGF+DDP组与DDP组比较,MDA的含量有所下降而SOD、NO和GSH-Px的活性有所升高,差异均有统计学意义(P0.01);而aFGF+DDP组与Control组比较,各生化和酶学指标含量或活性均未回落至正常水平,差异仍具有统学意义(P 0.01)。结论:aFGF对顺铂诱导的血管内皮细胞损伤具有一定的保护作用。aFGF对顺铂血管毒性的保护作用可能通过与组织细胞的表面受体结合,抑制脂质过氧化,直接或间接拮抗自由基损伤,稳定细胞酶活性有关。
[Abstract]:Objective : To investigate the protective effect of acidic fibroblast growth factor ( aFGF ) on vascular endothelial cell injury induced by cisplatin ( DDP ) in vitro . Methods : The endothelial cells of rat vascular endothelial cells were obtained by tissue block method . The cells were cultured in 96 - well culture plates . After 24 hours of culture , the cell survival rate was determined by MTT assay . ( 3 ) After 24 h culture , the protective effects of aFGF on DDP - induced injury of vascular endothelial cells were observed . Results : ( 1 ) The inhibitory concentration ( IC50 ) of DDP on endothelial cells of vascular endothelial cells was increased . ( 2 ) After 24 h of the addition of 2.559 mg / L DDP , the activity of SOD , NO and GSH - Px was decreased and the difference was statistically significant ( P0.01 ) . Conclusion : aFGF has a protective effect on cisplatin - induced injury of vascular endothelial cells . The protective effect of aFGF on cisplatin - induced vascular endothelial cells may be related to the inhibition of lipid peroxidation , direct or indirect antagonism of free radical damage and stable cell enzyme activity by binding to the surface receptors of tissue cells .

【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R346

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