三种大鼠急性肺损伤模型的比较

发布时间：2017-12-31 07:23

  本文关键词：三种大鼠急性肺损伤模型的比较　出处：《成都医学院学报》2016年01期 　论文类型：期刊论文

  更多相关文章： 急性肺损伤 盲肠结扎穿刺 腹腔注射 气管滴注

【摘要】：目的比较腹腔注射脂多糖(lipopolysaccharide,LPS)、气管滴注LPS和盲肠结扎穿刺(cecal ligation and puncture,CLP)诱导的3种急性肺损伤模型的差异。方法 70只成年SD大鼠随机分为7组:正常组、腹腔注射LPS组(10mg/kg,LPS-IP组)及其假手术组(SHAM-1);气管滴注LPS组(5mg/kg,LPS-IT组)及其假手术组(SHAM-2);盲肠结扎穿刺组(CLP)及其假手术组(SHAM-3)。于造模后24h,收集各组大鼠的动脉血用于血气分析,并通过二喹啉甲酸(bicinchoninic acid,BCA)蛋白测定法、血细胞计数板以及瑞氏-姬姆萨染色分别对肺泡灌洗液中的蛋白含量、细胞总数和类别进行统计,同时利用湿干比和苏木精-伊红(HE)染色法来评价肺组织的损伤程度。结果 LPS-IT组和LPS-IP组的肺灌洗液总蛋白浓度、总细胞数均高于正常组和假手术组(P0.05),湿干比明显增高(P0.05),PaO2低于正常组及相应的假手术组(P0.05);LPS-IT组和LPS-IP组肺组织有明显病理变化,包括肺水肿和肺泡壁增厚,炎性细胞浸润。CLP组未见明显改变。结论在急性肺损伤的3种大鼠模型中,LPS-IT组和LPS-IP组肺损伤较为严重,CLP组未见明显肺损伤。提示腹腔注射LPS及气管给予LPS更适用于建立急性肺损伤模型以及后续应用药物的动物实验。
[Abstract]:Objective to compare the intraperitoneal injection of lipopolysaccharide (lipopolysaccharide, LPS), tracheal instillation of LPS and cecal ligation and puncture (cecal ligation and puncture, CLP) the difference between the 3 models of acute lung injury induced. Methods 70 adult SD rats were randomly divided into 7 groups: normal group, intraperitoneal injection of LPS group (10mg/kg group, LPS-IP) and the sham operation group (SHAM-1); tracheal instillation of LPS group (5mg/kg, group LPS-IT) and sham operation group (SHAM-2); cecal ligation and puncture (CLP) group and sham operation group (SHAM-3). After modeling 24h, blood gas analysis of arterial blood was collected for rats, and by two quinoline carboxylic acid (bicinchoninic acid BCA) protein determination method, blood cell counting plate and Giemsa staining respectively on alveolar lavage fluid protein content, cell number and categories of statistics, the ratio of wet and dry and hematoxylin eosin (HE) to evaluate the degree of injury of lung tissue staining results. The total protein concentration in bronchoalveolar lavage fluid of LPS-IT group and LPS-IP group, the total cell number was significantly higher than that in normal group and sham operation group (P0.05), wet dry ratio increased significantly (P0.05), PaO2 was lower than that in normal group and sham operation group (P0.05); the LPS-IT group and LPS-IP group had obvious pathological changes in lung tissue. Including pulmonary edema and alveolar wall thickening,.CLP group had no obvious change of infiltration of inflammatory cells. Conclusion in 3 kinds of rat model of acute lung injury in LPS-IT group and LPS-IP group were significantly more serious lung injury, lung injury was not found in the CLP. It is suggested that intraperitoneal injection of LPS and intratracheal instillation of LPS is more suitable for establishing the model of acute lung injury and the subsequent application of drugs in animal experiments.

【作者单位】： 成都医学院药学院;第三军医大学大坪医院野战外科研究所一室 创伤、烧伤与复合伤国家重点实验室;
【基金】：国家重点基础研究发展计划973项目(No:2012CB518102) 军队“十二五”重点项目(No:BWS11J038)
【分类号】：R-332;R563
【正文快照】： 创伤、烧伤与复合伤国家重点实验室(重庆400016)急性肺损伤(acute lung injury,ALI)由严重感染、休克、误吸等肺内外致病因素引发,临床主要表现为急性进行性加重的呼吸困难和难治性低氧血症,严重者可发展为急性呼吸窘迫综合征(acuterespiratory distress sydrome,ARDS)[1]。虽，

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