甘露消毒丹对CoxA16小鼠模型的影响

发布时间：2017-12-31 12:37

本文关键词：甘露消毒丹对CoxA16小鼠模型的影响　出处：《中药药理与临床》2017年02期 　论文类型：期刊论文

【摘要】：目的:探讨甘露消毒丹对柯萨奇病毒A16型(CoxA16)小鼠模型的影响。方法:7日龄ICR小鼠150只,随机分为正常组、模型组、阿昔洛韦组、甘露消毒丹组,每组25只;除正常组外,其余各组每只小鼠腹腔内注射20ul 107TCID50的CoxA16标准株原液进行造模,造模后的第5天,除正常组和模型组外,其余4组使用相应的药物干预治疗。观察小鼠临床表现;在光镜下观察肌肉组织形态学变化;用细胞增殖与毒性检测试剂盒(CCK-8)检测肌肉匀浆液对Hela细胞存活率的变化;电镜观察肌肉组织病毒颗粒的变化,采用ELISA法检测肌肉组织IL-6、IL-10、INF-γ的浓度。结果:与模型组比较,发现阿昔洛韦及甘露消毒丹(24.4g/kg、12.2g/kg、6.1g/kg)均可提高小鼠的生存率并使体重恢复到正常情况。HE染色和电镜结构表明甘露消毒丹(24.4g/kg、12.2g/kg、6.1g/kg)和阿昔洛韦均可减轻CoxA16病毒对肌肉的损伤,并减少肌肉内病毒的颗粒。通过肌肉组织匀浆液对Hela细胞存活率的变化表明甘露消毒丹能抑制病毒在肌肉内的生长。ELISA检测结果发现阿昔洛韦与甘露消毒丹均能减少IL-6的浓度,并提高IL-10、INF-γ的表达。结论:甘露消毒丹对CoxA16感染的小鼠有保护作用,能够抑制并杀死体内的CoxA16病毒。
[Abstract]:Objective: To investigate the Ganluxiaodu Dan on Coxsackie virus type A16 (CoxA16) mice model. Methods: 7 day old ICR 150 mice were randomly divided into normal group, model group, acyclovir group, Ganluxiaodu Dan group, 25 rats in each group; the other groups except the normal group, each mouse intraperitoneal injection 20ul 107TCID50 CoxA16 standard strain solution model was made fifth days after modeling, in addition to the normal group and model group, treatment of the corresponding drug intervention in other 4 groups. Clinical manifestations were observed; morphological changes were observed in muscle tissue under light microscope; cell proliferation and toxicity detection kit (CCK-8) detection of muscle homogenates of survival rate of Hela cells; changes in muscle tissue electron microscopic observation of virus particles, IL-10 muscle IL-6, ELISA method was used to detect the concentration of INF-, gamma. Results: compared with the model group, and acyclovir found sweet dew disinfection Dan (24.4g/kg, 12.2g/kg, 6.1g /kg) can improve the survival rate of mice and weight recovery to normal.HE staining and electron microscopy showed that the structure of Ganluxiaodu Dan (24.4g/kg, 12.2g/kg, 6.1g/kg) and A Silowe can reduce the damage of CoxA16 virus on the muscle, and reduce muscle virus particles. The muscle tissue homogenate of Hela cell survival rate showed that growth the results of.ELISA Ganluxiaodu Dan can inhibit the virus in muscles found A Silowe and Ganluxiaodu Dan can reduce the concentration of IL-6, and increase IL-10, INF- expression. Conclusion: Ganluxiaodu Dan on CoxA16 infected mice has a protective effect, can inhibit and kill the CoxA16 virus in the body.

【作者单位】：湖南中医药大学中医学院;
【基金】：国家自然科学基金资助项目(81173188)
【分类号】：R285.5;R-332
【正文快照】： 手足口病(Hand-foot-and-mouth disease,HFMD)是由多种肠道病毒引起的一种常见的传染性疾病,其中由柯萨奇病毒A16型(CA16)和肠道病毒71型(EV71)引起的占很大比例,通常多发生在5岁以下的儿童[1]。肠道病毒属的柯萨奇病毒、肠道病毒71型(EV71)和个别埃可病毒均可致手足口病,目前

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