成纤维细胞生长因子受体1在大鼠各组织中的表达

发布时间：2017-12-31 18:05

本文关键词：成纤维细胞生长因子受体1在大鼠各组织中的表达　出处：《新乡医学院学报》2016年11期 　论文类型：期刊论文

【摘要】：目的研究成纤维细胞生长因子受体1(FGFR1)mRNA及蛋白在大鼠各组织中的表达。方法采用实时定量荧光聚合酶链式反应测大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉等组织中FGFR1 mRNA表达;采用酶联免疫吸附法及免疫组织化学法检测各组织中FGFR1蛋白的表达。结果 FGFR1 mRNA和蛋白在大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉中均有表达,主要分布于细胞膜。FGFR1 mRNA在大鼠脂肪、肝脏、心脏、血管、肾脏、肌肉中表达量分别为2.623 0±0.524 9、1.003 0±0.077 2、6.755 0±0.278 4、1.613 0±0.320 1、4.833 0±0.421 5、6.123 0±0.348 7,FGFR1蛋白在以上各组织中的表达量分别为(10.670 0±1.197 0)、(0.356 0±0.105 5)、(0.860 0±0.163 9)、(5.152 0±0.801 4)、(0.186 0±0.046 7)、(2.110 0±0.161 9)μg·g-1,FGFR1 mRNA在各组织中的表达从高至低依次为心脏、肌肉、肾脏、脂肪、血管和肝脏;FGFR1蛋白在各组织中的表达量从高至低依次为脂肪、血管、肌肉、心脏、肝脏、肾脏。FGFR1 mRNA和蛋白在各组织中的表达量比较差异均有统计学意义(P0.05,P0.01)。结论 FGFR1在大鼠各主要组织器官中均有表达,主要分布于细胞膜,且在各组织中的表达存在一定差异。
[Abstract]:Objective to study the expression of fibroblast growth factor receptor (FGFR1) mRNA and protein in rat tissues. Methods the fat, liver and heart of rats were measured by real-time quantitative fluorescent polymerase chain reaction. FGFR1 mRNA was expressed in blood vessel, kidney, muscle and other tissues. The expression of FGFR1 protein was detected by enzyme-linked immunosorbent assay (Elisa) and immunohistochemistry. Results FGFR1 mRNA and protein were detected in fat, liver, heart, blood vessel and kidney of rats. The expression of FGFR1 mRNA in rat fat, liver, heart, blood vessel, kidney and muscle was 2.623 0 卤0.524 9. 1.0030 卤0.077 2 卤6.755 0 卤0.278 4 卤1.613 0 卤0.320 1 卤4.833 0 卤0.421 5. The expression of FGFR1 protein in the above tissues was 10.6700 卤1.197). (0.356 0 卤0.105 5) (0.8600 卤0.163 9) (5.152 0 卤0.8014). The expression of FGFR1 mRNA in each tissue was from high to low. The expression of FGFR1 mRNA was 2.1100 卤0.161.9 渭 g 路g-1g. Muscle, kidney, fat, blood vessels and liver; The expression of FGFR1 protein in various tissues is followed by fat, blood vessel, muscle, heart and liver. Kidney. FGFR1 mRNA and protein expression in each tissue were significantly different (P0.05). Conclusion FGFR1 is expressed in the main tissues and organs of rats, mainly distributed in the cell membrane, and there are some differences in the expression of FGFR1 in different tissues.
【作者单位】：首都医科大学附属北京安贞医院心内科北京市心肺血管疾病研究所;
【基金】：国家自然科学基金资助项目(编号:81570388) 国家重点基础研究发展计划(973计划)资助项目(编号:2015CB554404) 北京市自然科学基金资助项目(编号:7142048)
【分类号】：R363
【正文快照】： 成纤维细胞生长因子受体(fibroblast growth fac-tor receptors,FGFRs)属受体酪氨酸激酶超家族成员,为体内重要的信号调节分子。它可以与相应成纤维细胞生长因子(fibroblast growth factors,FGFs)结合,引发靶细胞的多种反应,从而在许多生理过程中发挥重要作用,如细胞增殖、胚

【相似文献】