番石榴叶总黄酮促进糖尿病模型小鼠胰岛再生机制

发布时间：2017-12-31 20:21

本文关键词：番石榴叶总黄酮促进糖尿病模型小鼠胰岛再生机制　出处：《中国实验方剂学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:观察番石榴叶总黄酮对链脲佐菌素(streptozocin,STZ)糖尿病模型小鼠胰腺组织中胰腺十二指肠同源盒因子-1(pancreas-duodenumhomeobox-1,PDX-1),神经元素3(neurogenin 3,Ngn3),NK6转录因子相关基因座1(NK6 transcription factor related locus 1,Nkx6.1)表达的影响,探讨其促进胰岛β细胞再生的机制。方法:将STZ糖尿病模型小鼠随机分为模型组和治疗组,治疗组包括番石榴叶总黄酮低、高剂量组及二甲双胍组,另设10只正常小鼠为正常组。番石榴叶总黄酮低、高剂量组按小鼠体重分别以番石榴叶总黄酮0.198,0.396 g·kg~(-1)·d~(-1)灌胃,二甲双胍组以二甲双胍0.087 5 g·kg~(-1)·d~(-1)灌胃,正常组和模型组以等体积蒸馏水灌胃,2周后检测其空腹血糖、体重,苏木素-伊红(HE)染色观察胰岛病理形态学改变,实时荧光定量聚合酶链反应(Real-time PCR)检测胰腺组织内胚胎期胰岛β细胞发育相关转录因子PDX-1,Ngn3,Nkx6.1 mRNA相对表达量。结果:与正常组比较,STZ糖尿病模型小鼠胰腺组织中PDX-1,Ngn3,Nkx6.1表达量明显下降(P0.01);与模型组比较,番石榴叶总黄酮治疗组PDX-1,Ngn3,Nkx6.1 mRNA表达升高(P0.05)。结论:番石榴叶总黄酮能上调糖尿病小鼠胰腺组织中PDX-1,Ngn3,Nkx6.1的表达;胰岛β细胞再生机制可能与PDX-1,Ngn3,Nkx6.1 mRNA表达的上调有关。
[Abstract]:Objective: to observe the effect of total flavonoids in guava leaves on streptozocin. STZ) Pancreas-duodenumhomeobox-1 (PDX-1) was found in pancreatic tissues of diabetic mice. Nerve element 3 neurogenin 3 Ngn3). NK6 transcription factor-related locus 1NK6 transcription factor related locus 1nkx6.1). Methods: STZ diabetic mice were randomly divided into model group and treatment group. The treatment group included low total flavonoids in guava leaves, high dose group and metformin group. In addition, 10 normal mice were used as normal group. The total flavonoids in guava leaves were low, and in the high dose group, the total flavonoids in guava leaves were 0.198 according to the body weight of the mice. 0.396 g 路kg ~ (-1) 路d ~ (-1))) and metformin group (0.087 5 g 路kg ~ (-1) 路d ~ (-1)) were intragastrically perfused with metformin (0.087 5 g 路kg ~ (-1)). The normal group and the model group were fed with distilled water of equal volume for 2 weeks. The fasting blood glucose, body weight and hematoxylin eosin (HEH) staining were used to observe the pathomorphological changes of pancreatic islets. Real-time PCR real-time PCR was used to detect the expression of PDX-1and Ngn3, a transcription factor associated with the development of pancreatic 尾 cells in pancreatic tissues at embryonic stage. Results: compared with the normal group, PDX-1ngn3 was detected in pancreatic tissue of Nkx6.1 diabetic mice. The expression of Nkx6.1 decreased significantly (P 0.01). Compared with the model group, the total flavonoids of guava leaves were treated with PDX-1 and Ngn3. Conclusion: total flavonoids of guava leaves can up-regulate the expression of PDX-1Ngn3Nkx6.1 in pancreatic tissues of diabetic mice. The mechanism of islet 尾 cell regeneration may be related to the up-regulation of Nkx6.1 mRNA expression.
【作者单位】：天津医科大学研究生院;天津市南开医院;天津市医药科学研究所;
【基金】：天津市卫生和计划生育委员会中医中西医结合科研项目(2015042)
【分类号】：R285.5;R-332
【正文快照】： [网络出版地址]http://kns.cnki.net/kcms/detail/11.3495.R.20170308.0946.030.html[网络出版时间]2017-03-08 9:46近年来,糖尿病患病率逐年攀升,根据国际糖尿病联盟(IDF)统计,2011年全球糖尿病患者人数已达3.7亿,随着我国社会、经济的发展及人口老龄化进程的加速,糖尿病的患

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