基于thermoTRP对骨癌痛大鼠模型虚寒型冷痛的机理研究

发布时间：2018-01-01 01:07

本文关键词：基于thermoTRP对骨癌痛大鼠模型虚寒型冷痛的机理研究　出处：《中华中医药学刊》2017年07期 　论文类型：期刊论文

【摘要】：目的:基于观察肿瘤骨转移大鼠转移灶周围骨组织TRPA1和TRPM8表达变化以及冷刺激缩足阈值改变,阐释骨癌痛虚寒型冷痛病机。方法:健康4月龄SD雄性大鼠45只,体质量440~470 g,随机分成模型组(仅造模,无药物干预)、模型-通道阻滞剂干预组(造模2周后应用TRPA1、TRPM8拮抗剂干预的大鼠);空白组(正常健康大鼠,无药物干预)。分别于造模前1周、造模后2、4、6、8周,在3组中随机选择5只大鼠,测定冷缩足潜伏期;并在造模后8周处死大鼠,取转移灶周围骨组织样本,观察组织病理切片形态,并检测TRPA1、TRPM8基因量及蛋白表达水平。结果:造模后2周,模型组大鼠出现明显的冷刺激痛敏,至造模后8周其冷刺激痛阈仍维持较低水平,应用TRPA1、TRPM8通道阻断剂后,骨癌痛大鼠的冷刺激痛阈能显著恢复至接近正常水平。结论:TRPA1、TRPM8通道蛋白表达的上调参与构建了骨癌痛大鼠虚寒型冷痛的病机基础。
[Abstract]:Objective: to observe the changes of TRPA1 and TRPM8 expression and the threshold of cold stimulation in bone tissue of rats with bone metastasis. Methods: 45 healthy 4-month-old SD male rats with a body mass of 440 ~ 470g were randomly divided into model group (model only, no drug intervention). Model-channel blocker intervention group (rats treated with TRPA1 / TRPM8 antagonist for 2 weeks); In the blank group (normal healthy rats, no drug intervention), 5 rats were randomly selected in the 3 groups at 1 week before model making and 2 minutes and 6 weeks after model making, and the latent period of cold contraction foot was measured. The rats were killed 8 weeks after the model. The bone tissue samples around the metastatic focus were taken to observe the histopathological sections and the expression of TRPA 1 TRPM8 gene and protein. Results: 2 weeks after the establishment of the model, the expression of TRPM8 gene was detected. The pain threshold of cold stimulation in the model group was maintained at a low level after 8 weeks, and the pain threshold of cold stimulation was maintained at a low level after the application of TRPA1 / TRPM8 channel blocker. Conclusion the up-regulation of the expression of TRPA _ 1 and TRPM8 channel protein is involved in the pathogenesis of cold type cold pain in rats with bone cancer pain.
【作者单位】：南京中医药大学附属医院创伤骨科;
【基金】：国家自然科学基金项目(81573993) 江苏省基础研究计划(自然科学基金)项目(BK20151598)
【分类号】：R273;R-332
【正文快照】： 癌痛是临床恶性肿瘤患者最常见的症状之一,也是破坏患者生存质量的重要因素。原发性或者转移性肿瘤引起的疼痛会随着肿瘤的发展不断加重。随着癌症诊疗技术的进步,癌症患者的生存时间明显延长,如何更有效地控制癌痛在肿瘤姑息治疗领域日益关键。骨癌痛是癌症痛的典型代表,也是

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